TY - JOUR ID - 103926 TI - Molecular detection of Theileria spp in sheep and vector ticks in Fasa and Kazeroun areas, Fars Province, Iran JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Yaghfoori, S. AU - Razmi, G. AU - Heidarpour, M. AD - Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran AD - Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Y1 - 2013 PY - 2013 VL - 68 IS - 2 SP - 159 EP - 164 KW - Theileria KW - Semi KW - Nested PCR KW - Sheep KW - Fars province KW - Iran DO - 10.7508/ari.2013.02.011 N2 - A molecular survey was performed for identification Theileria spp in sheep and ticks during from 2010-2011 in Fasa and Kazeroun areas, Fars province, Iran. A total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. The prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. The collected ticks were separated into tick pools with five ticks according to their species and sex. Then, the salivary glands were dissected out in 0.85% saline solution under stereomicroscope. The boold and tick salivary glands samples were examined by using semi-nested PCR. The Theileria spp infection was observed in 46% of blood smears, while 76 % of blood samples were positive by using semi-nested PCR. T. ovis, T. lestoquardi and mixed infection were detected in 43 (43%), 3 (3%) and 30 (30%) of positive samples, respectively. Any significant difference was not observed between the frequency of Theileria spp infection in sheep of Kazeroun and Fasa areas. In the present study, the most prevalent ticks were R. turanicus 48.8% and followed by H. a. anatolicum 42.2% and H. marginatum 8.8%. The results were shown that one pool belong to salivary glands of H. turanicus were infected with T. ovis. Based on the obtained results, it is concluded that T. ovis have high prevalence with compared to T. lestoquardi and also, R. turanicus could be the vectors T. ovis in this area UR - https://archrazi.areeo.ac.ir/article_103926.html L1 - https://archrazi.areeo.ac.ir/article_103926_3234daace26cd7c7e44bc033ef184ffd.pdf ER -