TY - JOUR ID - 103729 TI - Optimal Timing of Specimen Collection after Rash Onset for Diagnosis of Measles IgM Antibody JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Mokhtari Azad, T. AU - Naseri, M. AU - Yavari, P. AU - Gooya, M.M. AU - Esteghamati, A. AU - Hamkar, R. AD - Y1 - 2005 PY - 2005 VL - 60 IS - 1 SP - 1 EP - 10 KW - Measles KW - IgM Capture EIA KW - Optimal time DO - 10.22092/ari.2005.103729 N2 -   Antibody detection is the most versatile and commonly used method for measles diagnosis. Detection of specific IgM antibodies in a single serum specimen collected within the appropriate time after rash onset can provide a good presumptive diagnosis of current or recent measles infection and is the test of choice for rapid diagnosis of measles cases. So, optimal timing for collection of a single serum specimen to diagnose measles by IgM capture Enzyme Immunoassay (EIA) was evaluated. 399-paired sera were tested for measles IgM antibody. 149-paired sera were measles IgM negative. Two hundred fifty paired sera had at least one IgM positive. 223-paired sera were positive in both first and second samples. 19-paired sample were negative in first and positive in second sample and 8-paired sera were positive in the first and negative in the second samples. 85% and 100% of first specimens within 7 and 7-21 and 94% of all second samples were IgM positive at 28th days after rash onset, respectively. Analysis of data indicates that a single serum specimen collected between 7 to 27 days after rash onset can be used to diagnose most cases of measles with an IgM capture EIA.   UR - https://archrazi.areeo.ac.ir/article_103729.html L1 - https://archrazi.areeo.ac.ir/article_103729_c0197333612dc597ab8e4a10e8d89794.pdf ER -