Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Cloning and Expression of Com1 and OmpH Genes of Coxiella burnetii in Periplasmic Compartment of Escherichia coli with the Aim of Recombinant Subunit Vaccine Production
341
347
EN
H.
Bakhteyari
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
hanieh.bakhteyari74@gmail.com
R.
Jahangiri
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
ranajahan1234@gmail.com
N.
Nazifi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
narges.nazifi@gmail.com
A.
Kakanezhadifard
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
a.kakanezhadi@gmail.com
Z.
Soleimani
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
zahra.12soleimani@gmail.com
Ali
Forouharmehr
Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
forouharmehr.a@lu.ac.ir
S.
Azadi Chegeni
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
azadi.shiva1990@gmail.com
A.
Jaydari
Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
jaydari.a@lu.ac.ir
10.22092/ari.2018.122911.1233
<em>Coxiella burnetii</em>is an obligate and gram-negative bacteria causing query fever (Q fever) disease, despite the importance of Q fever, there is no universal vaccine against this disease. Therefore, application of the recombinant subunit vaccines which use <em>Com1</em> and <em>OmpH</em> as immunogenic proteins can be useful in this regard. To perform the current project, <em>Com1</em> and <em>OmpH</em> genes were amplified by polymerase chain reaction (PCR) method, then, the PCR products were purified by DNA precipitation technique. In order to clone, first, both genes along with the pET-22b(+) vector were digested by <em>NcoI</em> and <em>XhoI</em> enzymes and then, <em>Com1</em> and <em>OmpH</em> genes were ligated in linear vectors by T4 DNA ligase. The recombinant vectors were transformed in BL21 (DE3) strain of <em>Escherichia coli</em> and expression was induced by 1 mM Isopropyl β-D-1-thiogalactopyranoside. Expression of <em>Com1</em> and <em>OmpH</em> was investigated using 12% Sodium dodecyl sulfate polyacrylamide gel electrophoresis. Finally, both proteins were purified by Ni-NTA columns and consequently confirmed by western blotting. The results of assessing 1% agarose gel showed that PCR amplification, DNA precipitation, and digestion of both genes were successfully performed.Theresults of colony PCRs and sequencing revealed that <em>Com1</em> and <em>OmpH</em> were correctly cloned in pET-22b(+) vector. Finally, the results of expression, purification, and western blotting of both proteins showed thatBL21 (DE3) strain of <em>Escherichia coli</em>could be able to express <em>Com1</em> and <em>OmpH</em> proteins<strong>.</strong> Based on the collected data, it seems that <em>Escherichia coli</em> as an affordable and simple host can be applied to express <em>Com1</em> and <em>OmpH</em> genes. It should be mentioned that products of the present project can be examined as recombinant subunit vaccines against Q fever.
Coxiella burnetii,Com1,ompH,E.coli,subunit vaccine
https://archrazi.areeo.ac.ir/article_120197.html
https://archrazi.areeo.ac.ir/article_120197_e470f4f2647e67245acbe3a34c52e6f3.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
349
355
EN
H.
Yaghoubi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
kamran.nazemi@gmail.com
A.
Ghalyanchilangeroudi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
arashghalyanchi@gmail.com
Vahid
Karimi
0000-0001-7815-5553
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
vkarimi@ut.ac.ir
S. A.
Ghafouri
Department of clinical sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
s_ali_ghafouri@yahoo.com
M.
Hashemzadeh
Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
hashemzadehma@gmail.com
H.
Hosseini
Department of Poultry Diseases, Islamic Azad University, University of Tehran, Tehran, Iran
hosseini.ho@gmail.com
M. H.
Fallah
0000-0002-1221-7771
Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
mhf2480@yahoo.com
F.
Sadat Mousavi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
fatememousavi7715@gmail.com
H.
Najafi
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
hamideh.najafi.1988@gmail.com
10.22092/ari.2018.116786.1176
Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to describe the epidemiology of GCOVs in avian species. The present study was conducted to detect GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight different bird species ((Chickens (<em>Gallus gallus</em>), Pheasant (<em>Phasianus colchicus</em>), Turkey (<em>Meleagris gallopavo</em>), Partridge (<em>Perdix perdix</em>), Quail (<em>Coturnix coturnix</em>), Duck (Anas platyrhynchos), Goose (Anserini),and Guinea fowl (Numididae)) were collected, the viral RNA was extracted, the RT-PCR was performed using QIAGEN one step RT-PCR kit and the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates. Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran. According to our results, GCOVs are circulating in different avian species, and further studies are needed to isolate these viruses and evaluate their pathogenesis.
Gamma coronavirus,molecular detection,Bird Parks,Iran,Phylogenetic analysis
https://archrazi.areeo.ac.ir/article_120191.html
https://archrazi.areeo.ac.ir/article_120191_c6fae8a431f025b13db442d5fbc00516.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Efficacy of CpG-ODN Administration Routes on Humoral Responses against Newcastle disease in Broilers
357
364
EN
A.
Talebi
0000-0001-6476-0488
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
a.talebi@urmia.ac.ir
S.
Arky-rezai
DVM
Graduated, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
s_arkirezaee@yahoo.com
10.22092/ari.2018.120544.1196
Un-methylated cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) has been considered as a powerful vaccine adjuvant and recognition of CpG-ODN by chicken leukocytes promotes their ability to fight against infections. In our study, efficacy of different routes of CpG-ODN application as an adjuvant on immune responses (antibody titer together with leukogram) following vaccination against Newcastle disease (ND) has been evaluated in broiler chickens (Ross-308). The results indicated that routes of CpG-ODN administration influence immune responses and comparison effectiveness of CpG-OND delivery routes showed that group vaccinated by eye-drop application had the highest antibody titer than that of the group injected intramuscularly (im) and the difference was significant (<em>p</em> = 0.04) on day 35 of age. Antibody titer of the group treated with Clone 30 plus CpG-ODN via eye-drop route was higher than that of the group vaccinated with clone 30 alone on days 28 and 35 of age and the difference was significant (<em>p</em> = 0.04). Co-administration of both vaccine and CpG improved outcome of leukogram of the chickens on days 21 to 42 of age and among the treated groups, WBC of the group received both vaccine and CpG by eye-drop route significantly (<em>p </em>< 0.05) differed from that of the group vaccinated with clone 30 alone on days 28 and 35 but not on day 42 of age. Average final body weight of the control group did not significantly differ from those of the treated groups at end of the experiment. In conclusion, co-administration of ND vaccine plus CpG-ODN via eye-drop route improves immune responses.
CpG,Antibody titer,ND,broilers,Vaccine administration route
https://archrazi.areeo.ac.ir/article_120192.html
https://archrazi.areeo.ac.ir/article_120192_0c0a75d32af904a75422e82f7552835b.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Seroprevalence Investigation of Newcastle Disease in Rural Poultries of the Northern Provinces (Golestan, Gilan, and Mazandaran) of Iran
365
373
EN
A.
Alemian
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
abbasalemian@yahoo.com
S. A.
Pourbakhsh
Department of poultry Research and Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
a.pourbakhsh@srbiau.ac.ir
A.
Shoushtari
Department of poultry Research and Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
hamid1342ir@yahoo.com
H.
Keyvanfar
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
h-keyvanfar@srbiau.ac.ir
10.22092/ari.2017.116669.1175
Rural poultry farming is common in the Northern provinces. Similar to commercial poultry, rural poultry is susceptible to most infectious diseases. In addition, by increasing the density of poultry farming, the probability of disease incidences has been increased. Newcastle disease is the most highly infectious disease which is endemic in Iran and causes outbreaks among commercial and rural poultry every year. The present study aimed to investigate the prevalence and virus circulation of Newcastle disease among rural poultry in Northern provinces of Iran. In the current study, 70 villages in 3 provinces (20, 30, and 20 villages in Mazandaran, Golestan, and Gilan, respectively) and a total of 1,374 birds (600, 400, and 374 birds in Mazandaran, Golestan, and Gilan, respectively) were sampled. Each village was regarded as an epidemiological unit. In the present study, birds of 67 (96%) villages were positive (presence of antibodies against Newcastle disease virus), including 28 (93.3%), 19 (95%), and 20 (100%) villages in Golestan, Mazandaran, and Gilan, respectively. Moreover, out of 1,374 birds, 616 (45%) of them were seropositive against Newcastle disease virus with 242 (41%), 159 (39.8%), and 211 (56%) samples in Mazandaran, Golestan, and Gilan, respectively. According to the results of the current study, the seroprevalence rate was reported to be high in both villages and birds. Such a high seroprevalence rate was indicative of the continuous exposure of the rural poultry to Newcastle virus and high virus circulation rate in the mentioned provinces which could result in the dissemination of the disease to commercial farms. Consequently, the implementation of proper control and care programs (e.g., vaccination of native poultry) can facilitate the reduction of Newcastle disease prevalence.
Seroprevalence,Newcastle disease,Poultry,Northern provinces,HI
https://archrazi.areeo.ac.ir/article_120190.html
https://archrazi.areeo.ac.ir/article_120190_a4840bb0dbeaf716cb0729fe5f2b36d9.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Identification of Non-Tuberculosis Mycobacteria by Line Probe Assay and Determination of Drug Resistance Patterns of Isolates in Iranian Patients
375
384
EN
Morteza
Karami-Zarandi
Departemant of Microbiology, School of Medicin, Tehran University of Medical Sciences, Tehran, Iran
mkz.7052@yahoo.com
A.
Bahador
Department of Microbiology, School of medicine, Tehran University of Medical Sciences, Tehran, Iran
ab.bahador@gmail.com
S.
Gizaw Feysia
Department of infectious Disease, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences
seifugizaw@yahoo.com
J.
Kardan-Yamchi
Department of Microbiology, School of medicine, Tehran University of Medical Sciences, Tehran, Iran
jkardan666@yahoo.com
M.
Hasan-nejad
Department of infectious Disease, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences
m.hasannejad11@yahoo.com
H.
Vosough
MD Pathologist, Nikan General Hospital, Tehran, Iran
hooman_voosough@yahoo.com
N.
Mosavari
0000-0002-3480-3376
RReference Laboratory for Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
nmosavari@gmail.com
M. M.
Feizabadi
Department of Microbiology, School of medicine, Tehran University of Medical Sciences, Tehran, Iran
mfeizabadi@tums.ac.ir
10.22092/ari.2019.127144.1372
The potentially pathogenic Non-Tuberculosis Mycobacteria (NTM) are emerging nowadays which result in pulmonary and non-pulmonary infections in human. This group of bacteria consists of at least 200 different species. While the pulmonary disease is the most common form of NTM infections, NTM can cause diffused infections as well as extrapulmonary infections in every organ, such as bone marrow, skin, eye, and brain. The NTM cause tuberculosis-like infections, therefore, correct identification of these Mycobacteria is necessary to avoid faulty treatment. Different species of NTM isolates were identified from clinical specimens using phenotypic methods and Line Probe Assay. Minimum Inhibitory Concentration for selected antibiotics was obtained by the broth micro-dilution method. Totally, 42 NTM isolates were identified in this study. Moreover, the frequency of NTM between all positive mycobacterium cultures was estimated at 12%. The most common Rapidly Growing Mycobacteria included<em> Mycolicibacterium fortuitum</em> (30.9%)<em>, Mycobacterium abscessus</em> (7.1%), and <em>Mycobacterium chelonae</em> (2.3%), whereas <em>Mycobacterium simiae </em>(40.4%)<em>, Mycobacterium kansasii</em> (16.6%), and <em>Mycobacterium avium</em> complex (2.3%) were the most recurring among the Slowly Growing Mycobacteria. Amikacin, clarithromycin, and ciprofloxacin were the most effective antibiotics against isolated NTM. The NTM isolates are frequently being separated from Iranian patients, and are mostly resistant to the wide spectrum of antibiotics. Correct identification and determination of antibiotic susceptibility can be helpful in the healing process of the patients who suffer from non-tuberculosis mycobacterial infections.
Drug Resistance Patterns,Line Probe Assay,Non-Tuberculosis Mycobacteria
https://archrazi.areeo.ac.ir/article_120667.html
https://archrazi.areeo.ac.ir/article_120667_22df4ee80dd6aaea120fe557c570dabe.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Bradykinin-Potentiating Factors of Venom from Iranian Medically Important Scorpions
385
394
EN
H. R.
Goudarzi
Central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
hr.goudarzi@rvsri.ac.ir
Z.
Salehi Najafabadi
Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
zahra.salehi@live.com
A.
Movahedi
Central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
M.
Noofeli
Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
10.22092/ari.2019.123404.1249
The venom of animals, including snakes, scorpions, and spiders is a complex combination of proteins, peptides, and other biomolecules as well as some minerals. Among the biomolecules of some animal’s venom, small peptides that lack disulfide bands known as Non-Disulfide Bridge Peptides (NDBPs) potentiate the bradykinin by preventing the conversion of angiotensin 1 to angiotensin 2 using the mechanism of inhibiting the Angiotensin-Converting Enzyme activity and finally reducing the blood pressure in the victims. This feature of the NDBPs of animal’s venom is suggested as the potential of biological drugs. This study aimed to isolate venom components of three species of Iranian medically important scorpions and study the bradykinin potentiating effect of them. The scorpion specimens were collected from the venomous animals and antivenom production department of Razi Vaccine and Serum Research Institute, Karaj, Iran. Moreover, venom extraction was performed by electrical shock (5 volts). The obtained liquid venom of three species specimens was frozen and lyophilized immediately and then preserved in a cool and dried place. The isolation of the venom components for each scorpion was carried out using high-performance liquid chromatography. The obtained ranges of venom fractions (zones) were tested on isolated tissues of guinea-pig ileum and rat uterus using organ bath instrumentation in several replicates. The bioassays resulted in the peptides, including Z<sub>1</sub> and Z<sub>2 </sub>regions in the venom fractionsof the <em>Hottentotta saulcyi</em>, Z<sub>2</sub> in <em>Odontobuthus doriae</em>, as well as Z<sub>2</sub> and Z<sub>3</sub> in <em>Mesobuthus eupeus</em> demonstrated bradykinin potentiating effect. It is concluded that Bradykinin Potentiating Factors were traceable in the venom of all three scorpion species. Therefore, these venoms have the therapeutic potential to exploit biological-based drugs.
Biologic drugs,Bradykinin-potentiating factors,hypertension,Scorpion venom
https://archrazi.areeo.ac.ir/article_120200.html
https://archrazi.areeo.ac.ir/article_120200_525b6ecf1d1279b41e3daa5bbaad0e99.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Evaluation of Influence of Zeolite/Collagen Nanocomposite (ZC) and Hydroxyapatite (HA) on Bone Healing: A Study on Rabbits
395
403
EN
D.
Faraji
Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
darab.faraji@gmail.com
Alireza
Jahandideh
assistant professor /science and research university of tehran
dr.jahandideh@gmail.com
A.
Asghari
0000-0001-5152-8807
Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
dr.ahmad.asghari@gmail.com
A.
Akbarzadeh
Universal Scientific Education and Research Network (USERN), Tabriz, Iran
dr.akbarzadeh2010@gmail.com
S.
Hesaraki
Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
hesarakisaeed@yahoo.com
10.22092/ari.2018.121308.1211
Bone healing is still a great challenge in orthopedic surgery and clinical practice. There is a dearth of research investigating the effect of Zeolite/Collagen (ZC) nanocomposite on bone regeneration. In the present study, a critical segmental defect of the rabbit femur was repaired using defects in femurs repaired by ZC nanocomposite, and the effects were examined histologically. In total, 45 rabbits at seven months of age weighing 3.5 kilograms were utilized in this study. After making the bone defects, all animals were randomized into three groups (n=15). In a normal control group (NC), a defect was created, no intervention was made, and the skin incision was sutured. On the other hand, in the ZC group, the nanocomposite of ZC was placed into the created defect. In the hydroxyapatite group (HA), the hydroxyapatite was placed into the created defect. The samples were collected on days 15, 30, and 45 postoperatively and assessed histopathologically. The mean scores of the index of the union were compared and considerable alterations were observed in this regard in the experimental groups (P<0.05). The values of the index of spongiosa demonstrated that on day 15, it was the highest in the ZC group (2.2) and lowest in the HA and NC groups (0.6). Moreover, the values of the index of bone marrow demonstrated no noticeable alteration among the values of the index of bone marrow in the experimental groups (P>0.05). The findings of this study demonstrated that ZC nanocomposite might be considered for reconstruction in bone damages. It seems the ZC nanocomposite bears a crucial capability in the reconstruction of bone damages and might be used as a biological frame in bone damages.
Bone Regeneration,Histopathological Evaluation,nanocomposite,Zeolite/Collagen Nano Particles,Rabbit
https://archrazi.areeo.ac.ir/article_120193.html
https://archrazi.areeo.ac.ir/article_120193_73c68a7c130a843a0ec00845378f42d9.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Extraction, Purification, and Characterization of Trypsin Obtained from the Digestive System of Yellowfin Seabream (Acanthopagrus latus)
405
411
EN
F.
Namjou
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
faeze.namjou@yahoo.com
S.
Yeganeh
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
skyeganeh@gmail.com
R.
Madani
Department of Proteomics & Biochemistry section Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
madanirasool@gmail.com
H.
Ouraji
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran
h.ouraji@sanru.ac.ir
10.22092/ari.2018.122854.1229
The development of the marine aquaculture industry has led to the generation of significant amounts of fish wastes. Marine farm wastes exert adverse effects on the surrounding area of the cages. On the other hand, wastes of fish and other aquatic animals are regarded as major sources of valuable natural bioactive compounds, including enzymes, proteins, bioactive peptides, oil, amino acids, collagen, gelatin, calcium, biopolymers, and water-soluble minerals. To investigate the potential of marine fish waste, the whole digestive system of yellowfin seabream (<em>Acanthopagrus latus</em>) was extracted for extraction and identification of trypsin enzyme. Fish (179.93±93.67 g; 184±28.17 cm) were caught from the Persian Gulf and stored at -20 °C. Yellowfin seabream were dissected and their whole digestive systems were removed. Samples were thoroughly washed with distilled water and purified through defatting using acetone and ammonium sulfate precipitation. The following issues were assessed: the total and specific activity of trypsin, protein determination, molecular weight, enzyme activity and stability in different pH values and temperatures. The obtained results indicated that specific activity and protein content of trypsin enzyme were 4.4 U and 3.4 mg/ml, respectively. The molecular weight of 23 kDa was reported for trypsin using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method. Maximum activity and stability of trypsin were observed at 60°C and 45°C, respectively. Trypsin demonstrated maximum activity and stability at a pH value of 8.0. In general, the results of the current study suggested that trypsin extracted from the digestive system of yellowfin seabream has considerable potential for industrial applications, such as the food industry, owing to its characteristics and stability under alkaline conditions.
Enzyme purification,Yellowfin seabream (Acanthopagrus latus),Trypsin,Fish waste
https://archrazi.areeo.ac.ir/article_120196.html
https://archrazi.areeo.ac.ir/article_120196_049caae371b0a48c1d9e1fa7fa0487d5.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Determination of the Effective Dose of Curcumin alone and in Combination with Antimicrobial Peptide CM11 on Promastigote Forms of Iranian Strain of L. major (MRHO / IR / 75 / ER)
413
422
EN
G.
Aqeele
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
ghasikaqeele@gmail.com
P.
Shayan
0000-0002-4666-3235
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
pshayan@ut.ac.ir
E.
Ebrahimzadeh
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
eebrahimzade@ut.ac.ir
M.
Mohebali
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
mmohebali@hotmail.com
S.
Khalili
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
s.khalili@ut.ac.ir
10.22092/ari.2018.122300.1222
Zoonotic cutaneous leishmaniasis t caused by <em>Leishmania major</em> is spread in focal areas of more than 90 countries in the tropics, subtropics, and southern Europe. In the absence of any effective vaccine, the only means to treat and control leishmaniasis is conventional medication. Glucantime is the first choice of anti-leishmanialdrug, has serious side effects like high toxicity, exorbitant cost, problems with the administration and development of resistance. Curcumin is the active component from the rhizome of herb Curcuma longa, possessing many pharmacological and biological activities with antiprotozoal and anti-proliferative effects which make it a good alternative to existing therapy. Antimicrobial peptides like CM11, a small peptide consisting of 11 amino acids, are also novel potential drugs against at least wide spectrum of microbial organisms. The aim of this study was to evaluate the effect of curcumin alone and in combination with CM11 on promastigote form of <em>L. major</em> (MRHO / IR / 75 / ER) for 12h and 24h <em>in vitro</em>. The results of Giemsa staining showed that the morphology of the flagellum and cell shape increased changed with increasing concentration of curcumin (5 µM, 10 μM, 20 μM, 40 μM and 80 μM). MTT and Trypan blue results demonstrated that the promastigotes were susceptible against curcumin in dose and time dependent manner, while CM11 alone at concentration of 8 µM as well as in combination with 10 and 20 µM curcumin had no significant effect on promastigotes. Our results revealed that curcumin can provide a new curative candidate against cutaneous leishmaniasis.
Curcumin,Antimicrobial Peptide CM11,Promastigote,Leishmania. major,Glucantime
https://archrazi.areeo.ac.ir/article_120194.html
https://archrazi.areeo.ac.ir/article_120194_290408b679713358d4b799a994a991fb.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Identification and Determination of the Geographical Distribution of Freshwater Snails in Lorestan, Iran
423
431
EN
M. H.
Razi Jalali
0000-0002-8160-8522
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
mh.jalali@scu.ac.ir
M.
Mirzaei
0000-0003-1829-1478
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
dr_mirzaie_mo@yahoo.com
F.
Jahangiri Nasr
0000-0001-7218-7511
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
ersi.jahangiri@yahoo.com
H.
Sharifi
0000-0002-9008-7618
Department of Epidemiology, Faculty of Public Health, Kerman University of Medical
Sciences, Kerman, Iran
hamidsharifi@uk.ac.ir
10.22092/ari.2018.123286.1244
Snails are creatures present in various ecosystems that, in addition to being present in human surroundings, some of them are also important in veterinary medicine and medicine as the intermediate hosts of Digenean trematodes. The present study was conducted to identify and determine the geographical distribution of freshwater snails and investigate the relationship of variables, such as season and geographical region, with snail species and dispersion in Lorestan in the west of Iran. A total of 4400 samples of freshwater snails were collected using the multistage sampling method (i.e., stratified, cluster, and randomized) from 110 points in five geographical regions in four seasons and then identified based on their morphological characteristics by diagnostic keys. The ArcGIS software (version 10.3) was used to evaluate the spatial distribution of the freshwater snails. In this study, seven species of freshwater snails were identified in six families belonging to six genera, namely <em>Melanopsis doriae</em> (6.30% of the variation in species), <em>Theodoxus doriae</em> (5.55%), <em>Bithynia tentaculata</em> (43.22%, the dominant species), <em>Physa acuta</em> (24.98%), <em>Lymnaea truncatula</em> (9.75%), <em>Gyraulus euphraticus</em> (8.18%), and <em>Lymnaea gedrosiana</em> (2.02%). The geographic distribution of freshwater snails was recorded across five regions in 22 points per region for every season. The spatial distribution maps showed that the distribution of freshwater snails varies according to region and season (P<0.001). The obtained results revealed the effects of season and geographical region on the distribution and population density of snails in the province. These data can be used for the implementation of control programs against parasitic diseases in the region, including trematodes.
identification,freshwater snail,geographical distribution,Lorestan
https://archrazi.areeo.ac.ir/article_120199.html
https://archrazi.areeo.ac.ir/article_120199_e5e9c65145667d3291005952654c2cf9.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Determination of CD Markers Profile of the Cell Line Infected by S15 Vaccine Strain of Theileria annulata Schizont Using RT-PCR Analysis
433
438
EN
H.
Modirrousta
Department of wildlife research, Razi vaccine and serum research institute, agriculture research, education, and extension organization (AREEO), Karaj, Iran
h.modirrousta@rvsri.ir
G.
Habibi
0000.0003.0052.4608
Department of parasite vaccine research and production, Razi vaccine and serum research institute, agriculture research, education, and extension organization (AREEO), Karaj, Iran
g.habibi@rvsri.ac.ir
P.
Shayan
Department of parasitology, Veterinary Faculty, Tehran University, Tehran, Iran
A.
Mirjalili
Department of biotechnology, Razi vaccine and serum research institute, agriculture research, education, and extension organization (AREEO), Karaj, Iran
K.
Esmaeilnia
Department of Parasite Vaccine Research and Production, Razi vaccine and serum research institute, agriculture research, education, and extension organization (AREEO), Karaj, Iran
10.22092/ari.2018.123081.1237
<span>The aim of this study was to identify the cell surface cluster of differentiation (CD) markers of the cell lines infected by<em> Theileria annulata</em> schizont. The CD molecules are very useful for the characterization of cells and different subpopulations of leukocytes. They are usually recognized by specific antibodies using flow cytometry and immunohistochemistry. In the current study, we applied reverse transcriptase-polymerase chain reaction (RT-PCR) to define the profile of cell surface markers in a cell line infected by an attenuated S15 vaccine strain of <em>T. annulata</em> schizont and a new laboratory-established cell line infected by a non-attenuated form. In order to determine the specific markers that can be used for excluding the non-attenuated cell lines, the characterization of the <span>surface protein</span>s profile of the S15 vaccine cell line is important. The RT-PCR was carried out by specifically designed primers using a panel of seven bovine CD markers, as well as beta-actin as an internal control house-keeping gene. We showed that both of the examined cell lines had a consistent expression of CD4, CD5, CD11a, CD14, CD43, and CD45 markers. However, the specific finding in this study was the expression of B-cell markers CD79a and CD5 by the newly-transformed cell line. On the other hand, CD5 as a marker for B-cell subset was expressed by S15 vaccine strain. In conclusion, we consider CD79a surface protein as a new marker for the cell lines infected by non-attenuated <em>T. annulata</em> schizont, while the cell lines infected by the vaccine strain do not express this marker. In addition, the identification of CD marker expression based on the RT-PCR assay might be a suitable and appropriate alternative technique for flow cytometry.</span>
CD marker,CD79a,RT-PCR,Theileria annulata,vaccine
https://archrazi.areeo.ac.ir/article_120198.html
https://archrazi.areeo.ac.ir/article_120198_36bce0a1f9b3d7372abea8c97c9fe2e6.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Detection and Isolation of H9N2 Subtype of Avian Influenza Virus in House Sparrows (Passer domesticus) of Ahvaz, Iran
439
444
EN
Z.
Boroomand
Department of avian health and diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
z.boroomand@scu.ac.ir
M.
Mayahi
0000-0002-4084-0990
Department of avian health and diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
m_mayahi@yahoo.com
H.
Hosseini
Department of avian health and diseases, Faculty of Veterinary Medicine, Islamic Azad University, Tehran branch, Tehran, Iran
hosseini.ho@gmail.com
S.
Valadbeigi
Department of avian health and diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
valadbeigi.s91@gmail.com
10.22092/ari.2019.122504.1223
Avian influenza (AI) is an acute infectious disease with worldwide significance causing extensive economic losses in the poultry industry. Avian influenza viruses (AIVs) belong to the family Orthomyxoviridae and categorized in the genus influenza virus A. These viruses have been isolated from more than 100 species of free-living birds. Migratory birds are considered as reservoirs for AIVs and are the major agents responsible for global outbreaks. The Passeriformes are found in most parts of the world and cover a variety of habitats from rural to urban areas. House sparrows are members of the family Passeridae and due to their free flying, are strongly associated with seabirds, indigenous, and industrial poultry. The aim of this study was to determine the role of house sparrows in AIV (H9N2) circulation in the Ahvaz region. The intestinal and tracheal samples were taken from 200 sparrows around Ahvaz during 2017. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed using specific primers in order to detect M and H9 genes of AIVs. The positive specimens in the PCR for the M gene were inoculated into 9-11-day-old embryonated chicken eggs via the allantoic fluid. The results showed that 11 out of 200 samples were positive for the two genes of M and H9. According to the findings of the present study, house sparrows are infected with H9N2 and pose a threat to commercial poultry. These birds may play a significant role in the transmission of AIV between wildlife and domestic animals. Therefore, this issue is important to be considered in preventive measurements.
Ahvaz,Avian Influenza,house sparrows,Iran,molecular detection
https://archrazi.areeo.ac.ir/article_120195.html
https://archrazi.areeo.ac.ir/article_120195_637471ea570c663553bb4942d21d9892.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS)
435
439
EN
M.
Hematyar
Department of Chemistry, Imam Khomeini International University (IKIU), Qazvin, Iran
hematyar_marjan@yahoo.com
A.
Es-haghi
0000-0001-6533-3656
Department of Physico Chemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
a.eshaghi@rvsri.ac.ir
M.
Soleimani
Department of Chemistry, Imam Khomeini International University (IKIU), Qazvin, Iran
m-soleimani@hotmail.com
10.22092/ari.2018.122150.1219
The current research aimed to quantify melittin (MEL) in Iranian honey bee (<em>Apis mellifera meda</em>) venom. To this end, a liquid chromatography-electrospray ionization<strong>-</strong>ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin is the main toxic peptide of honey bee venom with various biological and pharmacological activities. It was extracted with pure water from the bee venom samples. The analyses were performed on XBridge BEH300 C4 column using a gradient method with the mobile phase consisting of ultrapure water and acetonitrile (containing 0.1% formic acid). Signals of the melittin were recorded with the selected reaction monitoring (SRM) mode, which is a quantitative approach capable of quantifying analyte peptides with high sensitivity and. The mass spectrum of MEL was obtained in the positive ion mode and the quantification analysis was performed using precursor to product ion transition of m/z 570.2/669.9. This method demonstrated good linearity (R<sup>2</sup>˃0.997) in the range of 1-100 µg mL<sup>-1</sup>, with a limit of quantification (LOQ) of 1.0 µg mL<sup>-1</sup>. The content of MEL in Iranian honey bee venom accounts for 43–55% of total dry weight. This method can be used to evaluate the quality and authenticity of bee venom samples for different therapeutic applications of MEL.
Apis mellifera meda,bee venom,Melittin,Peptide,LC-ESI-IT-MS/MS
https://archrazi.areeo.ac.ir/article_120201.html
https://archrazi.areeo.ac.ir/article_120201_dbe360a7b75247a80ffcb1158d8126a1.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
74
4
2019
12
01
Characterization and Pattern of Culling in Goats
441
446
EN
M.
Didarkhah
Faculty of Agriculture Sarayan, University of Birjand, Birjand, Iran
masooddidarkhah@birjand.ac.ir
M.
Vatandoost
Department of Agriculture, Payame Noor University
moosavatandoost@gmail.com
E.
Dirandeh
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran
dirandeh@gmail.com
N.
Dadashpour Davachi
0000-0001-9478-9775
Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
navid.d.davachi@gmail.com
10.22092/ari.2019.125298.1301
In order to describe the proportion and pattern of culling in commercial goatherds, this survey was carried out in an industrialized goatherd in Torbat-e-Jam, Iran, over a period of 18 years from 1996 to 2013. In total, the data of 3945 goats were used in this study. Finally, out of all samples, 499 (12%) goats were culled. The involuntary culling was performed mainly due to shortage disorders (3.8%), viral disorders (3.3%), microbial diseases (2.8%), and other disorders (2.1%). Sheep pox was the most important reason (64%) for culling due to viral disorders. Tick paralysis was the most common parasitic disease that contributed to culling and responsible for 88% of parasitic disorders. On the other hand, enterotoxemia accounted for 55% of microbial disorders is considered the most common cause of culling. The high proportion of culling due to shortage disorders, especially nutritional deficiencies should be considered the most important cause of culling. It requires precautionary measures and planning in order to reduce the aforementioned rate.
Culling,Goat herds,Microbial disease,VIRAL DISEASE
https://archrazi.areeo.ac.ir/article_120354.html
https://archrazi.areeo.ac.ir/article_120354_f1679c2da60086094eaa4e0b677850ea.pdf