Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
Organic Circulation and persistence of Foot – and – Mouth disease virus type O in guinea pig
67
73
EN
M.
Eslampanah
Department of Pathology, Razi Vaccine & Serum Research Institute, Karaj, Iran
H.
Mahravani
Department of FMD vaccine, Razi Vaccine & Serum Research Institute, Karaj, Iran
M.H.
Hablolvarid
Department of Pathology, Razi Vaccine & Serum Research Institute, Karaj, Iran
H.
Isadi
Department of FMD vaccine, Razi Vaccine & Serum Research Institute, Karaj, Iran
M.
Sotudeh
Department of FMD vaccine, Razi Vaccine & Serum Research Institute, Karaj, Iran
F.
Jirani
Department of FMD vaccine, Razi Vaccine & Serum Research Institute, Karaj, Iran
F.
Talebloo
Department of Pathology, Razi Vaccine & Serum Research Institute, Karaj, Iran
10.22092/ari.2010.103852
Since, the most susceptible laboratory animal to Foot–and–Mouth disease virus (FMDV) is guinea pig, and then one milliliter of FMDV type (O) concentrations of 106-106.5 TCID50was inoculated intradermally (ID) to 10 plantar surface of guinea pig (right side). Guinea pig adapted virus has been prepared after generalization phase, and then one milliliter of virus was inoculated intradermally to 30 guinea pigs in 5 groups (each consist of six). Samples from different organs including, heart, lung, liver, spleen, pancreas, tongue and plantar epithelium, retropharyngeal and inguinal lymph nodes were collected on days 2, 4, 14, 30, 60 post inoculation (PI) and kept in transport media and sent to FMD department of Ref lab. According to the results of this study, plantar epithelium and tongue as well as lung have been detected to be the original sites for virus survival. In addition, the highest rate of the presence of virus can be seen on 4 days and 14 days PI. By the way there was no sign of presence of the virus in any organs on days 30 and 60 PI.
Foot – and – Mouth disease virus,type O,ELISA,PCR,Guinea pig
https://archrazi.areeo.ac.ir/article_103852.html
https://archrazi.areeo.ac.ir/article_103852_c4960ec0c87acdf92d6094ce58dd3ef4.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
Detection of Mycoplasma synoviae infection in broiler breeder farms of Tehran province using PCR and culture methods
75
81
EN
F.
Elhamnia
M.
Banani
G.R.
Shokri
S.A.
Pourbakhsh
poursaba@yahoo.com
A.
Ashtari
10.22092/ari.2010.103853
Mycoplasma synoviae (MS) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the Iranian chicken industry especially breeder farms. The aim of this study was to use the MS specific PCR and culture methods in order to detect of M. synoviae from breeder farms where located in Tehran province. A total of 475 samples including choanal cleft, trachea, ovary and /or joint cavities from 23 broiler breeder farms of Tehran area were collected. Samples were cultured in PPLO broth media supplemented for MS isolation. The bacteria DNAs were extracted by phenol/chloroform method. Specific published primers amplify a 207 bp region of the 16S rRNA gene of MS were used for PCR method. Out of 475 samples, 146 cultures were shown positive and typical Mycoplasma colonies, 85 samples were also identified MS based on agglutination test with specific MS antiserum and the PCR method. A total of 122 samples, a band with 207 bp was shown as MS specific PCR product in electrophoresis. In addition to these 85 samples that were positives in both culture and PCR, 37 samples that had not grown in Mycoplasma media were positive in MS specific PCR. A total of 292 samples were negatives in both culture and PCR methods. 122 positive samples out of 475 samples (25.7%) were belonged to 7 breeder farms (30.4%). On conclusions, the MS infection of broiler breeder farms of Tehran area was confirmed truly. From the results, as the PCR method reduces the time consuming, an effectiveness and efficient for detection of M. synoviae infection of chicken breeder. It is then suggested that the PCR method could be an alternative method for culturing.
Mycoplasma synoviae,broiler breeder,PCR,Culture,Tehran
https://archrazi.areeo.ac.ir/article_103853.html
https://archrazi.areeo.ac.ir/article_103853_d87ced21ef6c7c00629d43b4c9486a0c.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
Serum Enzymes Studies in Scorpion (Hemiscorpius lepturus) Dose Related Envenomation in Rabbits
83
89
EN
Sh.
Teimourzadeh
S.
Mahmoodi Khatoonabadi
A.
Zare Mirakabadi
Gh.H.
Sabiri
10.22092/ari.2010.103854
Hemiscorpius lepturus is medically important scorpion species present in the south and southwest part of Iran, causing morbidity and mortality in children and adults. Unlike other scorpions studied so far, the venom of H. lepturus is highly cytotoxic, that can be a reason for its complexity in clinical manifestations in patients stung by this scorpion. Scarce studies showed the mechanism involved in envenomation by H. lepturus. In the present study, H. lepturus venom in three doses of 50, 500 and 1500 μg/kg were subcutaneously injected into three separate groups of rabbits. Electrocardiograms of all the rabbits were recorded during the experiment. Blood collection was carried out before, one and three hours after venom injection. Serum was used for determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), urea, creatinine, BUN (Blood Urea Nitrogen), creatine phosphokinase (CPK) and creatine kinase isoenzyme MB (CK-MB). In group 1 rabbits although some change were observed in serum biochemical parameters following venom injection but statistically the changes were not significant. In group 2 rabbits significant (P
H. lepturus,Scorpion venom,Electrocardiogram,Biochemical disorders,Serum enzymes
https://archrazi.areeo.ac.ir/article_103854.html
https://archrazi.areeo.ac.ir/article_103854_49473675d6a9312e783f3ead473f5be1.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
Ultrastructural and molecular studies on fat and thin macrocysts of Sarcocystis spp. isolated from naturally infected goats
91
97
EN
K.
Aghaeipour
A.
Dalimi
0000-0001-5591-9913
dalimi4@yahoo.com
Gh.R.
Motamedi
A.
Nouri
a.nouri@rvsri.ac.ir
10.22092/ari.2010.103855
There is considerable confusion concerning the structure and synonymity of macroscopic sarcocysts in goats. In the present work, ultrastructural and molecular study on fat and thin macrocysts of Sarcocystis isolated from naturally infected goats were investigated. Two forms of macrocystic sarcocysts were collected from fresh oesophagus, diaphragm, skeletal muscles and heart of slaughtered goats in abattoirs of Tehran and Ghazvin provinces of Iran. Then ultrastructural and molecular studies were carried out contemporaneous using PCR technique and transmission electron microscope respectively. Based on morphological examinations both cysts differ in shape with naked eye but they had the same wall structure with electron microscopy study. The 18S rRNA amplification by PCR showed a 637-bp band for both forms. The results of sequencing of PCR product of the cysts indicated these two kinds of cysts were identical to each other and also to Sarcocystis moulei. The present result, suggests that fat and thin cysts are the same and identical species.
Sarcocystis moulei,Goat,fat and thin sarcocyst,Ultrastructure,PCR,Iran
https://archrazi.areeo.ac.ir/article_103855.html
https://archrazi.areeo.ac.ir/article_103855_dfa1b16bf39121d3fac83a3195d9b7bc.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
Aflatoxin M1 contamination of raw and pasteurized milk produced in Sanandaj, Iran
99
104
EN
B.
Mohammadian
M.
Khezri
0000-0002-1200-0484
khezri1836@yahoo.com
N.
Ghasemipour
Sh.
Mafakheri
P.
Poorghafour Langroudi
10.22092/ari.2010.103856
This study was conducted to evaluate and compare the levels of aflatoxin M1 in raw and pasteurized milk samples during different seasons by Enzyme- Linked Immuno Sorbent Assay in Sanandaj, Iran. In 257 (94.49%) out of 272 milk samples the presence of aflatoxin M1 was detected in concentrations ranging between 0.007 and 115.930 ng/l. AFM1 level in 12 (4.4%) of positive samples were higher than the maximum tolerance limit (50 ng/l) accepted by Iran and European Union countries. Statistical evaluations showed that the differences between raw and pasteurized samples were not significant (p
Milk,aflatoxin,Measurement,ELISA
https://archrazi.areeo.ac.ir/article_103856.html
https://archrazi.areeo.ac.ir/article_103856_909a376a705893a4ea73e23f77085a92.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
An Unusual Occurrence of Actinobacillosis in Heifers and Cows in A Dairy Herd in Tehran suburb-Iran
105
110
EN
N.
Atyabi
A.
Khodakaram Tafti
T.
Taghipour Bazargani
Gh.
Faghanizadeh
10.22092/ari.2010.103857
An unusual occurrence of actinobacillosis was diagnosed in 4 heifers aged 8-15 months and 2 cattle in a dairy herd with 190 Iranian Holstein breed. Anorexia, dysphagia, drooling of normal or foodtinged saliva and presence of warts-like lesions on the dorsal surface of tongue shaft were observed in a 15-month-old heifer without showing protrusion of tongue or presenting woody tongue and no involvement of either sulcus lingualis or tongue base. In addition to tongue, soft tissues of oral cavity and pharyngeal region including lymph nodes, salivary glands and tonsils were contained multiple whitish nodules. Histopathologically, typical pyogranulomas of actinobacillosis contained radiating eosinophilic clubs surrounded by many neutrophils were diagnosed. Actinobacillus lignieresii was isolated from the lesions in pure culture. Clinical examination of other animals revealed the presence of different degrees of granulomatous abscesses in soft tissues including skin around mandibles in at least 3 heifers aged 8-11 months and 2 cattle. Due to 4 recent droughty years feeding the heifers, dry cows and low milk producing cattle by cheap oat and wheat straw mixed with plant awns could be the cause of this event.
Actinobacillosis,Pyogranulomatous lesions,Actinobacillus lignieresii,unusual occurrence,Cattle
https://archrazi.areeo.ac.ir/article_103857.html
https://archrazi.areeo.ac.ir/article_103857_4a6d94e3a9df5df141c0d17e76af4d1e.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
65
2
2010
11
01
A study on prevalence of Eimeria spp. infection in camels of Tabriz region
111
115
EN
M.
Yakhchali
Sh.
Athari
10.22092/ari.2010.103858
This investigation was carried out to determine the prevalence of Eimeria infections and the diversity of Eimeria species in camels of Tabriz region. The prevalence of Eimeria spp. infection and the intensity of faecal oocysts were determined in 164 camels using floatation and sporulation techniques. Faeces of 34 camels (20.73%) were infected with four Eimeria species including E. bactriani (52.42%), E. cameli (19.31%), E. pellerdyi (15.68%) and E. dromedarii (12.59%). Of infected animals, mixed infection was at most with four Eimeria species in 10.54% of camels. There was a significant difference between the prevalence of Eimeria infection in camels among different age groups (P
PREVALENCE,Eimeria spp,Camel,Tabriz
https://archrazi.areeo.ac.ir/article_103858.html
https://archrazi.areeo.ac.ir/article_103858_46c70ba0f64fd68b508cedc790233075.pdf