Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Expression of the VP2 gene of classical D78 infectious bursal disease virus in the methylotrophic yeast Pichia pastoris as a secretory protein
131
136
EN
D.
Shahbazzadeh
M.
Azizy
R.
Toroghi
M.R.
Seyfi Abad Shapoori
H.
Goudarzi
S.A.
Pourbakhsh
poursaba@yahoo.com
10.22092/ari.2006.103792
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. The expression of heterologous proteins in P.pastoris is fast, simple and inexpensive. In this study, VP2 encoding gene of classical D78 IBDV was amplified using reverse transcription (RT) polymerase chain reaction (PCR) and cloned into pPICZαA vector. Recombinant plasmid DNA was integrated into the chromosome of the transformed Pichia pastoris by electroporation and expressed protein identified by SDS- PAGE and western blotting. A recombinant protein was secreted into the supernatant from the yeast when induced with methanol. The expressed target protein in supernatant was bound with chicken anti IBDV Polyclonal antibodies. Western blotting with antibodies against D78 IBDV indicated that the recombinant VP2 protein retained its antigenicity. The concentration of secreted VP2 protein was 0.67mg/l.The production of recombinant VP2 protein indicated that P. pastoris was an efficient secreted expression system for D78 IBDV.
IBDV,Secreted expression,VP2,Pichia pastoris
https://archrazi.areeo.ac.ir/article_103792.html
https://archrazi.areeo.ac.ir/article_103792_cb39394aea74225e2474f825f25cfe3f.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia
137
141
EN
E.
Arefian
M.H.
Roostaei
T.
Bamdad
H.
Soleimanjahi
0000-0003-1931-7801
soleim_h@modares.ac.ir
A.
Ghaemi
10.22092/ari.2006.103793
Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nucliec acid . BALB/c mice were inoculated with HSV-1 and infected mice ganglia were applied to detect HSV-1 TK gene. Amplification of TK fragment in mice ganglia was considered as an indicator of viral latency. The results were suggested that TK PCR can be used as a reliable diagnostic tool and it is a specific and highly sensitive method for the detection of HSV genome in trigeminal ganglia.
Herpes simplex virus,Latency,Trigeminal ganglia,Thymidine kinase
https://archrazi.areeo.ac.ir/article_103793.html
https://archrazi.areeo.ac.ir/article_103793_65db1cbfd86f59080fe7fe2171bd67fb.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Prevalence of Echinococcus granulosus and other intestinal helminths of stray dogs in Mashhad area, Iran
143
148
EN
A.R.
Kamrani
K.
Sardari
G.R.
Razmi
10.22092/ari.2006.103794
In order to determine prevalence of Echinoccocus and other intestinal helminths infection one hundred stray dogs were examined in Mashhad area, Khorasan province of Iran. Helminths were collected from intestinal contents obtained at the time necropsy. Ninety one percent of the dogs were infected by different helminths. The prevalence of Echinococcus granulosus was (22%). Eighteen (81%) of infected dogs had (1-100) worm burden and 3(13%) and 1(4%) had (100-1000) and (>1000) worm burdens, respectively. The most heavily infected dog had 10,000 worms in the intestinal contents. Dogs were divided into three age group of 3 months-2 years, 2-6 years and > 6 years. The prevalence of Echinococcus infection was not significant by sex and age factors. Other intestinal helminths infections were as follow: Dipylidium caninum (41%), Taenia hydatigena (37%), Taenia ovis (8%), Mesocestoides lineatus (6%), Taenia multiceps (3%), Joyeuxiella echinorhyncoides (1%) and observed nematodes were Toxocara canis (37%), Toxascaris leonina (6%) and Ancylostoma caninum (1%). There was no significant difference in parasite prevalence between male and female dogs. The highest prevalence of Toxocara canis and as Dipylidium caninum infection were observed in 3 months to 2 years of dogs and in as contrast, in Taenia hydatigena was in 6> years. (p
PREVALENCE,E. granulosus,Intestinal helminths,Dog,Mashhad
https://archrazi.areeo.ac.ir/article_103794.html
https://archrazi.areeo.ac.ir/article_103794_9780f37df2c8cc9625ddca30a6195106.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Studies on incidence and pathology of neoplasms of reproductive system of cull laying
149
157
EN
A.A.
Mahjoor
H.
Nili
10.22092/ari.2006.103795
This study was performed to determine the incidence and anatomo-pathological aspects of the reproductive system tumors in commercial laying hens. Post mortem examination of 1041 laying hens revealed neoplasms of the ovaries and the oviduct in 52 (4.99%) cases. From these, ovarian adenocarcinoma was found in 14 cases, granulosa cell tumor in 7 cases, theca cell tumor in 1 case and Marek's disease in 3 cases. Oviductal adenocarcinoam was observed in 17 cases, adenomatosis in 2 cases and leiomyoma in 8 cases. The macroscopic and light microscopic characteristics of these tumors were reported in details.
Neoplasm,Cull laying hens,reproductive system
https://archrazi.areeo.ac.ir/article_103795.html
https://archrazi.areeo.ac.ir/article_103795_c1ff8264ec718ef8462e3711fafb2767.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Detection of human papilloma viruses type 16 and 18 by PCR and RFLP in paraffin-embedded cervical cancer tissue specimens
159
161
EN
H.
Mahmoodi
H.
Mirshahabi
H.
Soleimanjahi
0000-0003-1931-7801
soleim_h@modares.ac.ir
Z.M.
Hassan
M.
Farhadi Langeroudi
Z.
Meshkat
10.22092/ari.2006.103796
Human papillomaviruses (HPVs) infect mucosal and cutaneous epithelia and cause malignant and benign neoplastic lesions. Human papillomaviruses cause 250000 deaths per year from cervical cancer most often in developing countries. This major public health problem make them important targets in the search for Human papillomaviruses detection methods that may play a role in cervical cancer screening. In the present study, we used a combination of polymerase chain reaction (PCR) amplification method and Restriction fragment length polymorphism (RFLP) analysis in order to identify HPV types in cervical cancer samples. Two of the five samples were HPV positive that one of them was HPV16 positive and the other was positive for HPV18. The method could facilitate the sensitive identification of a broad spectrum of genital Human papillomaviruses.
https://archrazi.areeo.ac.ir/article_103796.html
https://archrazi.areeo.ac.ir/article_103796_cc1e6c16132b8a70571587104ae98701.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Isolation and identification of Clostridium septicum from sheep-dung
167
172
EN
G.M.
Yousofbeigi
A.
Morshedi
M.
Hemmaty
M.
Fathi Najafi
10.22092/ari.2006.103797
To study the existence of Clostridium septicum as probable agent associated with currently occurrence of black quarter in Urmia area, sheep-dung samples were collected. The bacteriological examinations on suspicious gram positive anaerobic spore-bearing bacilli which are in Clostridia genus include isolation and biochemical identification were performed. The results showed 28 different types of Clostridia contain of Cl. Septicum (6 isolates), Cl. Perfringens (5 isolates), Cl. novyi (4 isolates), Cl. fallax (4 isolates), Cl. innoccum (3 isolates), Cl. subterminale (2 isolates), Cl. carnis (2 isolates), Cl. ramosum (1 isolate), Cl. bifformentanse (1 isolate). All six isolated of Cl.septicum were confirmed by indirect florescent antibody test while other species were negative. PCR was performed for confirmation of isolation by using designed primers with respect to a segment of hemolysin gene (alpha or lethal toxin). All six isolates showed 270-bp band on agarose gel electrophoresis, suggesting conserved sequence for lethal toxin in Cl. septicum isolates. In this work we have isolated different Cl.septicum from sheep-dung in Urmia and biochemical, indirect immunoflorescent antibody and polymerase chain reaction tests suggesting that PCR could be selected as specific and quick method for identification of this bacterium.
Clostridium septicum,Isolation,PCR,Alpha toxin
https://archrazi.areeo.ac.ir/article_103797.html
https://archrazi.areeo.ac.ir/article_103797_1ada0007921164a97679dea0ac56f93a.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Newly designed instrument for desalination: Comparison with dialysis bag
173
177
EN
F.
Noohseresht
H.
Zolfagarian
M.A.
Zare
10.22092/ari.2006.103798
All desalination methods which are currently used have limitations. A newly designed instrument by Zare A.M. was tested and compared with currently used method in Razi Vaccine and Serum Research Institute(RVSRI). Samples of anti snake venom for desalination was provided by department of anti-serum, RVSRI. Parameters included in this study were time and water consumption during desalination process. PH, conductivity, Total protein, potency and dry weight of samples. Results reveals that complete desalination process by our newly designed instrument consumes only 4.30 hours and 80 litters of purified water as compared to currently used method of dialysis bag with average of 120 hours of time and 1920 litters of purified water(P
Desalination,Ammonium sulphate removal,Anti,Snake venom,Desalination instrument
https://archrazi.areeo.ac.ir/article_103798.html
https://archrazi.areeo.ac.ir/article_103798_31b0c35e304cfa40e0d5bacf79e5e42a.pdf
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
61
3
2006
12
01
Uterine adenocarcinoma in a rabbit
179
181
EN
M.R.
Golami
H.M.
Hablolvarid
M.
Moharami
10.22092/ari.2006.103799
On necropsy of a 24 months age Dutch female rabbit the uterine horns seems enlarged with a nodular appearance. On the cut surface of the uterus as well as heart wall, white firm masses with a cauliflower- like surface was observed. No other important gross lesions were found in other organs. In histopathological examination of the uterus typical microscopic changes were those of adenocarcinoma. No metastases except in heart wall were found in lung, spleen and liver. In the heart most of the myocardial cells were replaced by tumor masses and the reminder were degenerative. Microscopic features of the metastases in the heart wall were similar to the primary neoplasm.
https://archrazi.areeo.ac.ir/article_103799.html
https://archrazi.areeo.ac.ir/article_103799_b9c7a90dab513dff78ee9b7e263fa0db.pdf