ORIGINAL_ARTICLE
A study on the genetic analysis of clinical isolates and vaccine strains of Bordetella pertussis by pulsed-field gel electrophoresis (PFGE)
Considering the circulation of Bordetella pertussis clinical strains among populations with high vaccination coverage, it is necessary to have a proper understanding of this bacterium causing whooping cough. Various techniques, which are available for studying B. pertussis, can facilitate a proper comparison between different populations. We genotypically analyzed a collection of two vaccine strains used for the production of killed pertussis vaccine during 2000-2014 at Razi Vaccine and Serum Research Institute, Karaj, Iran. Ten clinical and two reference (Tohama 1 and 18323) strains were used by means of pulsed-field gel electrophoresis (PFGE). The genetic profiles of the vaccine master and working seeds showed no significant changes in the frequency of fingerprint types in the vaccine strains; also, the homogeneity of the profiles was demonstrated. However, the clinical isolates showed heterogeneity in the genetic profiles. In addition, serotyping was performed with monoclonal antisera to agglutinogens 2 and 3. Analysis of the fimbriae showed that all ten clinical strains expressed Fim3.
https://archrazi.areeo.ac.ir/article_107502_5b3a340989b06980a268ba3e2a69a7b2.pdf
2016-12-01
219
225
10.22034/ari.2016.107502
Bordetella pertussis
DNA fingerprints
PFGE
XbaI
A.
Bahmanjeh
1
Department of Microbiology, Faculty of Veterinnary, Islamic Azad University of Karaj, Karaj, Iran
AUTHOR
P.
Khaki
khakipejvak53@gmail.com
2
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
AUTHOR
S.
Moradi Bidhendi
3
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
AUTHOR
R.
Hosseinpour
4
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
AUTHOR
M.
Noofeli
noofeli1234@yahoo.com
5
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
LEAD_AUTHOR
Advani, A., Donnelly, D., Hallander, H., 2004. Reference system for characterization of Bordetella pertussis pulsed-field gel electrophoresis profiles. J Clin Microbiol 42, 2890-2897.
1
Advani, A., Hallander, H.O., Dalby, T., Krogfelt, K.A., Guiso, N., Njamkepo, E., von Konnig, C.H., Riffelmann, M., Mooi, F.R., Sandven, P., Lutynska, A., Fry, N.K., Mertsola, J., He, Q., 2013. Pulsed-field gel electrophoresis analysis of Bordetella pertussis isolates circulating in Europe from 1998 to 2009. J Clin Microbiol 422, 428-451
2
Caro, V., Hot, D., Guigon, G., Hubans, C., Arrive, M., Soubigou, G., Renauld-Mongenie, G., Antoine, R., Locht, C., Lemoine, Y., Guiso, N., 2006. Temporal analysis of French Bordetella pertussis isolates by comparative whole-genome hybridization. Microbes Infect 8, 2228-2235.
3
Cummings, C.A., Brinig, M.M., Lepp, P.W., van de Pas, S., Relman, D.A., 2004. Bordetella species are distinguished by patterns of substantial gene loss and host adaptation. J Bacteriol 186, 1484-1492.
4
Mooi, F.R., Hallander, H., Wirsing von König, C.H., Hoet, B., Guiso, N., 2000. Epidemiological Typing of Bordetella pertussis Isolates: Recommendations for a Standard Methodology. Eur J Clin Microbiol Infect Dis 19, 174-181.
5
Mooi, F.R., van Oirschot, H., Heuvelman, K., van der Heide, H.G., Gaastra, W., Willems, R.J., 1998. Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in The Netherlands: temporal trends and evidence for vaccine-driven evolution. Infect Immun 66, 670-675.
6
Nar Otgun, S., Durmaz, R., Karagoz, A., Esen, B., Ertek, M., 2011. Pulsed-field gel electrophoresis characterization of Bordetella pertussis clinical isolates circulating in Turkey in 2001-2009. Eur J Clin Microbiol Infect Dis 30, 1229-1236
7
Schouls, L.M., van der Heide, H.G., Vauterin, L., Vauterin, P., Mooi, F.R., 2004. Multiple-locus variable-number tandem repeat analysis of Dutch Bordetella pertussis strains reveals rapid genetic changes with clonal expansion during the late 1990s. J Bacteriol 186, 5496-5505.
8
van Amersfoorth, S.C., Schouls, L.M., van der Heide, H.G., Advani, A., Hallander, H.O., Bondeson, K., von Konig, C.H., Riffelmann, M., Vahrenholz, C., Guiso, N., Caro, V., Njamkepo, E., He, Q., Mertsola, J., Mooi, F.R., 2005. Analysis of Bordetella pertussis populations in European countries with different vaccination policies. J Clin Microbiol 43, 2837-2843.
9
van Gent, M., Heuvelman, C.J., van der Heide, H.G., Hallander, H.O., Advani, A., Guiso, N., Wirsing von Konig, C.H., Vestrheim, D.F., Dalby, T., Fry, N.K., Pierard, D., Detemmerman, L., Zavadilova, J., Fabianova, K., Logan, C., Habington, A., Byrne, M., Lutynska, A., Mosiej, E., Pelaz, C., Grondahl-Yli-Hannuksela, K., Barkoff, A.M., Mertsola, J., Economopoulou, A., He, Q., Mooi, F.R., 2015. Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012. Eur J Clin Microbiol Infect Dis 34, 821-830.
10
van Loo, I.H., Heuvelman, K.J., King, A.J., Mooi, F.R., 2002. Multilocus sequence typing of Bordetella pertussis based on surface protein genes. J Clin Microbiol 40, 1994-2001.
11
World Health Organization, W., 2013. Pertussis.
12
World Health Organization, W., 2016. Immunization surveillance, assessment and monitoring.
13
Zhang, L., Xu, Y., Zhao, J., Kallonen, T., Cui, S., Xu, Y., Hou, Q., Li, F., Wang, J., He, Q., Zhang, S., 2010. Effect of vaccination on Bordetella pertussis strains, China. Emerg Infect Dis 16, 1695-1701.
14
ORIGINAL_ARTICLE
Characterization of reoviruses isolated from some broiler breeder flocks in Iran
Avian reoviruses (ARVs) are considered as an important cause of several diseases in poultry, particularlyarthritis and tenosynovitis. Tenosynovitis and arthritis, which are among the causes of chronic lameness inbreeder flocks, can result in reduced egg production and culling of breeder hens. In this study, the molecularcharacteristics of ARVs in some broiler breeder flocks were investigated in Iran. After RNA extraction of thefield samples, reverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify two regionsof ARVs for S1 and S4 genes. The positive samples were further analyzed by five restriction enzymes inrestriction fragment length polymorphism (RFLP) for determining the strains. The phylogenetic analysis of S1and S4 genes from the isolates indicated divergence into five and four major lineages, respectively. Thesequence analysis of S1 and S4 genes of ARVs revealed that most of the positive samples were closely related totenosynovitis-inducing ARVs (with less than 2% nucleotide divergence). Also, these samples were mosthomologous to S1133 strain, with 99.90% nucleotide and amino acid affinity.
https://archrazi.areeo.ac.ir/article_107507_e2a19ad71ff059c9dc976e757fd386e4.pdf
2016-12-01
227
234
10.22034/ari.2016.107507
Molecular characteristics
Avian reoviruses
Tenosynovitis
Breeder flocks
Iran
M.
Hedayati
hedayati@malayeru.ac.ir
1
Department of Animal Sciences, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran & Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
M.
Shoojadost
2
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
S.M.
Peighambari
3
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Ghalyanchi Langeroudi
4
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Banyai, K., Dandar, E., Dorsey, K.M., Mato, T., Palya, V., 2011. The genomic constellation of a novel avian orthoreovirus strain associated with runting-stunting syndrome in broilers. Virus Genes 42, 82-89.
1
Benavente, J., Martinez-Costas, J., 2007. Avian reovirus: structure and biology. Virus Res 123, 105-119.
2
Bruhn, S., Bruckner, L., Ottiger, H.P., 2005. Application of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines. J Virol Methods 123, 179-186.
3
Chiu, C.J., Lee, L.H., 1997. Cloning and nucleotide sequencing of the S4 genome segment of avian reovirus S1133. Arch Virol 142, 2515-2520.
4
Clark, F.D., Ni, Y., Collisson, E.W., Kemp, M.C., 1990. Characterization of avian reovirus strain-specific polymorphisms. Avian Dis 34, 304-314.
5
Dale, R.K.H., Villegas, P., Kleven, S.H., 1983. Characteristics and Pathogenicity of Two Avian Reoviruses Isolated from Chickens with Leg Problems. Avian Diseases 27, 255-260.
6
Harzandi, N., H., K., H., S.A., A., P.S., 2006. Molecular detection of avian reoviruses using RT and nested PCR in tissue samples of suspicious flock in some provinces of Iran. Iran J Vet Sci 3, 367-374.
7
Hedayati, M., Shojadoost, B., 7(2):135-142., P.S.M., 2013. Detection of avian reoviruses causing tenosynovitis in breeder flocks of Iran by reverse transcription-polymerase chain reaction (RT-PCR) and restriction enzyme fragment length polymorphism (RFLP). Iran J Vet Med
8
Hsu, H.W., Su, H.Y., Huang, P.H., Lee, B.L., Liu, H.J., 2005. Sequence and phylogenetic analysis of P10- and P17-encoding genes of avian reovirus. Avian Dis 49, 36-42.
9
Kant, A., Balk, F., Born, L., van Roozelaar, D., Heijmans, J., Gielkens, A., ter Huurne, A., 2003. Classification of Dutch and German avian reoviruses by sequencing the sigma C protein. Vet Res 34, 203-212.
10
Kort, Y.H., Bourogâa, H., Gribaa, L., Scott-Algara, D., Ghram, A., 2013. Molecular characterization of avian reovirus isolates in Tunisian. Virol J 10, 1-10.
11
Li, Y.-J., Tsoi, S.C.-M., Mannen, H., Shoei-lung Li, S., 2003. Phylogenetic Analysis of Vertebrate Lactate Dehydrogenase (LDH) Multigene Families. Journal of Molecular Evolution 57, 745-745.
12
Liu, H.J., Lee, L.H., Shih, W.L., Li, Y.J., Su, H.Y., 2004. Rapid characterization of avian reoviruses using phylogenetic analysis, reverse transcription-polymerase chain reaction and restriction enzyme fragment length polymorphism. Avian Pathol 33, 171-180.
13
Pu, J., Liu, X., Guo, Y., Cao, Y., Zhao, J., Zhang, G., 2008. Seroprevalence of avian reovirus in egg-laying chicken flocks in China. Avian Dis 52, 675-679.
14
Razmyar, J., Peighambari, S.M., 2008. Molecular characterization of Iranian infectious bursal disease viruses. Avian Dis 52, 665-669.
15
Shapouri, M.R., Kane, M., Letarte, M., Bergeron, J., Arella, M., Silim, A., 1995. Cloning, sequencing and expression of the S1 gene of avian reovirus. J Gen Virol 76 ( Pt 6), 1515-1520.
16
Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24, 1596-1599.
17
Wickramasinghe, R., Meanger, J., Enriquez, C.E., Wilcox, G.E., 1993. Avian reovirus proteins associated with neutralization of virus infectivity. Virology 194, 688-696.
18
Yin, H.S., Lee, L.H., 1998. Identification and characterization of RNA-binding activities of avian reovirus non-structural protein sigmaNS. J Gen Virol 79 ( Pt 6), 1411-1413.
19
Zhang, Y., Liu, M., Shuidong, O., Hu, Q.L., Guo, D.C., Chen, H.Y., Han, Z., 2006. Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus. Arch Virol 151, 1525-1538.
20
ORIGINAL_ARTICLE
Isolation of lytic bacteriophages against pathogenic Escherichia coli strains in poultry in the northwest of Iran
In this study, 90 internal organ samples of poultry with symptoms of colibacillosis were obtained from Maragheh poultry farms in East Azerbaijan, Iran. In total, 70 bacterial isolates were confirmed as Escherichia coli (E. coli) strains using standard biochemical tests, and antibiotic sensitivity was determined by the disk diffusion method. Antibiotics used in this study included ampicillin, penicillin, nitrofurantoin, tetracycline, amoxicillin, ciprofloxacin, nalidixic acid, and sulfamethoxazole (n=8). Ciprofloxacin showed the highest susceptibility, while the lowest susceptibility was observed with penicillin and amoxicillin. Among the bacterial isolates, 50% showed resistance to at least five antibiotics, and 10 isolates with multidrug resistance were selected for bacteriophage (phage) isolation against recent E. coli isolates using spot test and double-layer agar overlay technique. In addition, water samples for phage isolation were provided from rivers, poultry farm sewages, and an urban sewage treatment center. In total, eight phages were successfully isolated from the urban sewage treatment center (total: 10). After enrichment, purification and titration, phages were further concentrated by polyethylene glycol precipitation. Lowest and highest bacteriophage titers were determined to be 1.05×106 and 1.9×109 PFU/ml, respectively. Host range of the isolated phages was assayed by spot testing, and antibacterial effects against four E. coli isolates were observed in one of the isolated phage suspensions, which was introduced as the most potentiated agent for phage therapy. In the morphological analysis of the selected phage using an electron microscope, we observed a hexagonal head with a diameter of 95 nm and contractile tail length of 90 nm, which indicated its similarity to the Myoviridae family. In conclusion, results of this study showed that bacteriophages could be appropriate alternatives to combat pathogenic E. coli strains with antibiotic resistance in poultry. Considering the changeable antibacterial effects of bacteriophages against different isolates of extraintestinal avian pathogenic E. coli, it is suggested that future investigations be conducted regarding the efficacy of lytic phages against different bacterial strains for the effective control of the associated infections in this region of Iran.
https://archrazi.areeo.ac.ir/article_107508_245844acdd516983458491bb82328fcd.pdf
2016-12-01
235
244
10.22034/ari.2016.107508
Bacteriophage
Colibacillosis
Antibiotic resistance
Escherichia coli
Poultry
R.
Majdani
rahelehmajdani@yahoo.com
1
Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran
LEAD_AUTHOR
Adams, M.H., 1959. Bacteriophages, Interscience Publishers, New York.
1
Ahmadpour, S., Mardani, K., Tukmechi, A., 2016. Culture and molecular characterization of phages isolated from rainbow trout farms and sewage treatment plants and investigation of their effects on Yersinia ruckeri. Iranian J Fishe Sci 15, 267-280.
2
Atterbury, R.J., Van Bergen, M.A., Ortiz, F., Lovell, M.A., Harris, J.A., De Boer, A., Wagenaar, J.A., Allen, V.M., Barrow, P.A., 2007. Bacteriophage therapy to reduce salmonella colonization of broiler chickens. Appl Environ Microbiol 73, 4543-4549.
3
Bauer, A.W., Kirby, W.M.M., Sherris, J.C., Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 36.
4
Biswas, B., Adhya, S., Washart, P., Paul, B., Trostel, A.N., Powell, B., Carlton, R., Merril, C.R., 2002. Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium. Infect Immun 70, 204-210.
5
Chang, H.C., Chen, C.R., Lin, J.W., Shen, G.H., Chang, K.M., Tseng, Y.H., Weng, S.F., 2005. Isolation and characterization of novel giant Stenotrophomonas maltophilia phage phiSMA5. Appl Environ Microbiol 71, 1387-1393.
6
Chibani-Chennoufi, S., Sidoti, J., Bruttin, A., Dillmann, M.-L., Kutter, E., Qadri, F., Sarker, S.A., Brüssow, H., 2004. Isolation of Escherichia coli Bacteriophages from the Stool of Pediatric Diarrhea Patients in Bangladesh. J of Bacteriology 186, 8287-8294.
7
Clark, J.R., March, J.B., 2006. Bacteriophages and biotechnology: vaccines, gene therapy and antibacterials. Trends Biotechnol 24, 212-218.
8
Cooke, E.M., Breaden, A., Shooter, R.A., O'Farrell, S., 1971. Antibiotic sensitivity of escherichia coli isolated from animals, food, hospital patients, and normal people. The Lancet 298, 8-10.
9
Dabrowska, K., Opolski, A., Wietrzyk, J., Switala-Jelen, K., Godlewska, J., Boratynski, J., Syper, D., Weber-Dabrowska, B., Gorski, A., 2004. Anticancer activity of bacteriophage T4 and its mutant HAP1 in mouse experimental tumour models. Anticancer Res 24, 3991-3995.
10
Ewers, C., Li, G., Wilking, H., Kiessling, S., Alt, K., Antao, E.M., Laturnus, C., Diehl, I., Glodde, S., Homeier, T., Bohnke, U., Steinruck, H., Philipp, H.C., Wieler, L.H., 2007. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: how closely related are they? Int J Med Microbiol 297, 163-176.
11
Fujiwara, A., Fujisawa, M., Hamasaki, R., Kawasaki, T., Fujie, M., Yamada, T., 2011. Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages. Appl Environ Microbiol 77, 4155-4162.
12
Goodridge, L., Abedon, S.T., 2003. Bacteriophage Biocontrol and Bioprocessing: Application of Phage Therapy to Industry. SIM News 53.
13
Hawkins, C., Harper, D., Burch, D., Anggard, E., Soothill, J., 2010. Topical treatment of Pseudomonas aeruginosa otitis of dogs with a bacteriophage mixture: a before/after clinical trial. Vet Microbiol 146, 309-313.
14
Hendrix, R.W., 2003. Bacteriophage genomics. Curr Opin Microbiol 6, 506-511.
15
Housby, J.N., Mann, N.H., 2009. Phage therapy. Drug Discov Today 14, 536-540.
16
Hudson, B.H., Frederick, J.P., Drake, L.Y., Megosh, L.C., Irving, R.P., York, J.D., 2013. Role for cytoplasmic nucleotide hydrolysis in hepatic function and protein synthesis. Proc Natl Acad Sci U S A 110, 5040-5045.
17
Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M., Donoghue, A.M., 2002a. Prevention of Escherichia coli infection in broiler chickens with a bacteriophage aerosol spray. Poult Sci 81, 1486-1491.
18
Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M., Donoghue, A.M., 2005. Alternatives to antibiotics: utilization of bacteriophage to treat colibacillosis and prevent foodborne pathogens. Poult Sci 84, 655-659.
19
Huff, W.E., Huff, G.R., Rath, N.C., Balog, J.M., Xie, H., Moore, P.A., Jr., Donoghue, A.M., 2002b. Prevention of Escherichia coli respiratory infection in broiler chickens with bacteriophage (SPR02). Poult Sci 81, 437-441.
20
Jassim, S.A., Abdulamir, A.S., Abu Bakar, F., 2012. Novel phage-based bio-processing of pathogenic Escherichia coli and its biofilms. World J Microbiol Biotechnol 28, 47-60.
21
Jassim, S.A., Limoges, R.G., 2013. Impact of external forces on cyanophage-host interactions in aquatic ecosystems. World J Microbiol Biotechnol 29, 1751-1762.
22
Jun, J.W., Kim, J.H., Shin, S.P., Han, J.E., Chai, J.Y., Park, S.C., 2013. Characterization and complete genome sequence of the Shigella bacteriophage pSf-1. Res Microbiol 164, 979-986.
23
Karamoddini, M.K., Fazli-Bazzaz, B.S., Emamipour, F., Ghannad, M.S., Jahanshahi, A.R., Saed, N., Sahebkar, A., 2011. Antibacterial efficacy of lytic bacteriophages against antibiotic-resistant Klebsiella species. Scientific World J 11, 1332-1340.
24
Kittler, S., Fischer, S., Abdulmawjood, A., Glunder, G., Klein, G., 2013. Effect of bacteriophage application on Campylobacter jejuni loads in commercial broiler flocks. Appl Environ Microbiol 79, 7525-7533.
25
Krylov, V., Shaburova, O., Krylov, S., Pleteneva, E., 2013. A genetic approach to the development of new therapeutic phages to fight pseudomonas aeruginosa in wound infections. Viruses 5, 15-53.
26
Kudva, I.T., Jelacic, S., Tarr, P.I., Youderian, P., Hovde, C.J., 1999. Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol 65, 3767-3773.
27
Kutter, E., De Vos, D., Gvasalia, G., Alavidze, Z., Gogokhia, L., Kuhl, S., Abedon, S.T., 2010. Phage therapy in clinical practice: treatment of human infections. Curr Pharm Biotechnol 11, 69-86.
28
Lang, L.H., 2006. FDA approves use of bacteriophages to be added to meat and poultry products. Gastroenterology 131, 1370.
29
Lau, G.L., Sieo, C.C., Tan, W.S., Hair-Bejo, M., Jalila, A., Ho, Y.W., 2010. Efficacy of a bacteriophage isolated from chickens as a therapeutic agent for colibacillosis in broiler chickens. Poult Sci 89, 2589-2596.
30
Laxminarayan, R., Duse, A., Wattal, C., Zaidi, A.K.M., Wertheim, H.F.L., Sumpradit, N., Vlieghe, E., Hara, G.L., Gould, I.M., Goossens, H., Greko, C., So, A.D., Bigdeli, M., Tomson, G., Woodhouse, W., Ombaka, E., Peralta, A.Q., Qamar, F.N., Mir, F., Kariuki, S., Bhutta, Z.A., Coates, A., Bergstrom, R., Wright, G.D., Brown, E.D., Cars, O., 2013. Antibiotic resistance; the need for global solutions. Lancet Infectious Diseases 13, 1057-1098.
31
Mankiewicz, E., Kurti, V., Adomonis, H., 1974. The effect of mycobacteriophage particles on cell-mediated immune reactions. Can J Microbiol 20, 1209-1218.
32
Matinkhoo, S., Lynch, K.H., Dennis, J.J., Finlay, W.H., Vehring, R., 2011. Spray-dried respirable powders containing bacteriophages for the treatment of pulmonary infections. J Pharm Sci 100, 5197-5205.
33
Merril, C.R., Scholl, D., Adhya, S.L., 2003. The prospect for bacteriophage therapy in Western medicine. Nat Rev Drug Discov 2, 489-497.
34
Oliveira, A., Sereno, R., Azeredo, J., 2010. In vivo efficiency evaluation of a phage cocktail in controlling severe colibacillosis in confined conditions and experimental poultry houses. Vet Microbiol 146, 303-308.
35
Owens, J., Barton, M.D., Heuzenroeder, M.W., 2013. The isolation and characterization of Campylobacter jejuni bacteriophages from free range and indoor poultry. Vet Microbiol 162, 144-150.
36
Quinn, P.J., Carter, M.E., Markey, B.K., Cartel, G.R., 1994. Clinical Veterinary Microbiology, Mosby-Year book Europe Limited, Wolfe publishing Ltd. London, England.
37
Roy, P., Purushothaman, V., Koteeswaran, A., Dhillon, A.S., 2006. Isolation, Characterization, and Antimicrobial Drug Resistance Pattern of Escherichia coli Isolated from Japanese Quail and their Environment. The J of Applied Poultry Research 15, 442-446.
38
Sulakvelidze, A., Alavidze, Z., Morris, J.G., Jr., 2001.
39
Bacteriophage therapy. Antimicrob Agents Chemother 45, 649-659.
40
Tsonos, J., Oosterik, L.H., Tuntufye, H.N., Klumpp, J., Butaye, P., De Greve, H., Hernalsteens, J.P., Lavigne, R., Goddeeris, B.M., 2014. A cocktail of in vitro efficient phages is not a guarantee for in vivo therapeutic results against avian colibacillosis. Vet Microbiol 171, 470-479.
41
van den Bogaard, A.E., London, N., Driessen, C., Stobberingh, E.E., 2001. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. J Antimicrob Chemother 47, 763-771.
42
Wright, A., Hawkins, C.H., Anggard, E.E., Harper, D.R., 2009. A controlled clinical trial of a therapeutic bacteriophage preparation in chronic otitis due to antibiotic-resistant Pseudomonas aeruginosa; a preliminary report of efficacy. Clin Otolaryngol 34, 349-357.
43
Zhao, S., Maurer, J.J., Hubert, S., De Villena, J.F., McDermott, P.F., Meng, J., Ayers, S., English, L., White, D.G., 2005. Antimicrobial susceptibility and molecular characterization of avian pathogenic Escherichia coli isolates. Vet Microbiol 107, 215-224.
44
ORIGINAL_ARTICLE
A study on prevalence of some helminthic infections of the liver and lungs among ruminants in abattoir of Fars province, Iran
Zoonotic helminths are often transmitted to humans through domestic animals. This retrospective study was performed to estimate the prevalence of some important zoonotic helminthic infections of the liver and lung including echinococcosis, fasciolosis and dicrocoeliasis in Kazerun and Shiraz abattoirs, Fars, Iran, during 2011-2013. A total of 12381 sheep, 6473 cattle, 22847 goats, 66 camels, and 10 buffalos were analyzed with regard to liver and lung helminthic infections including hydatidosis, fasciolosis, and dicrocoeliasis in Kazerun, during March 2011-January 2013. Moreover, 121100 sheep, 23515 cattle, 81293 goats, and 69 camels were investigated for the mentioned infections in Shiraz abattoir during one year since March 2012. Kazerun abattoir: The prevalence rates of liver hydatid cysts were 0.86%, 2.1%, 0.76%, and 15.1% in sheep, cattle, goats, and camels, respectively, whereas the prevalence rates of pulmonary hydatid cysts were 0.89%, 2.36%, 0.9%, and 16.6% in the mentioned animals, respectively. Fasciolosis was detected in 0.33%, 1.65%, and 0.24% of condemnation livers of sheep, cattle, and goats, respectively. However, dicrocoeliasis was just prevalent in 0.004% of goat livers. Shiraz abattoir: the prevalence rates of liver hydatid cysts were 3.44%, 3.12%, 2.94%, and 2.9% in slaughtered sheep, cattle, goats, and camels, respectively. In addition, 4.54%, 4.33%, 4%, and 4.35% of the lung of the mentioned animals were infected with hydatid cysts, respectively. Prevalence rates of Fasciola spp in slaughtered sheep and cattle were 2.49% and 1.86%, respectively, and rate of D. dendriticum infection in slaughtered sheep, cattle, and goats were 0.026%, 0.91%, and 4%, respectively. As compared with reports from other studies, it seems that Fars is among the low-endemic regions regarding this type of infection.
https://archrazi.areeo.ac.ir/article_107509_79fc50606ed67cb6181299d708c0bd07.pdf
2016-12-01
245
251
10.22034/ari.2016.107509
Zoonoses
Helminthes
Abattoir
Fars
Iran
T.
Mohamadzadeh
1
Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
AUTHOR
S.
Shams
2
Fars Industrial Abattoir, Shiraz, Iran
AUTHOR
K.
Khanaliha
khanaliha.kh@iums.ac.ir
3
Research Center of Pediatric Infectious Diseases, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
M.H.
Marhamatizadeh
4
Department of food hygiene, School of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran
AUTHOR
A.
Vafa
5
Fars Veterinary Network, Shiraz, Iran
AUTHOR
Abdi, J., Karimi, S., Naserifar, R., 2013. Zoonotic parasitic diseases in Ilam Province, Western Iran. Afr J Microbiol Res 7, 2957-2960.
1
Ahmadi, N.A., 2005. Hydatidosis in camels (Camelus dromedarius) and their potential role in the epidemiology of Echinococcus granulosus in Iran. J Helminthol 79, 119-125.
2
Amin Pour, A., Hosseini, S.H., Shayan, P., 2012. The prevalence and fertility of hydatid cysts in buffaloes from Iran. J Helminthol 86, 373-377.
3
Ansari-Lari, M., Moazzeni, M., 2006. A retrospective survey of liver fluke disease in livestock based on abattoir data in Shiraz, south of Iran. Prev Vet Med 73, 93-96.
4
Arbabi, M., Hooshyar, H., 2006. Survey of echinococcosis and hydatidosis in Kashan region, central Iran. Iran J Pub Health 35, 75-81.
5
Borji, H., Azizzadeh, M., Kamelli, M., 2012. A retrospective study of abattoir condemnation due to parasitic infections: economic importance in Ahwaz, southwestern Iran. J Parasitol 98, 954-957.
6
Cunningham, A.A., 2005. A walk on the wild side--emerging wildlife diseases. BMJ 331, 1214-1215.
7
Daryani, A., Alaei, R., Arab, R., Sharif, M., Dehghan, M., Ziaei, H., 2006. Prevalence of liver fluke infections in slaughtered animals in Ardabil province, Northwestern Iran. J Anim Vet Adv 5, 408-411.
8
Ekong, P.S., Juryit, R., Dika, N.M., Nguku, P., Musenero, M., 2012. Prevalence and risk factors for zoonotic helminth infection among humans and animals - Jos, Nigeria, 2005-2009. Pan Afr Med J 12, 6.
9
Khanjari, A., Bahonar, A., Fallah, S., Bagheri, M., Alizadeh, A., Fallah, M., Khanjari, Z., 2014. Prevalence of fasciolosis and dicrocoeliosis in slaughtered sheep and goats in Amol Abattoir, Mazandaran, northern Iran. Asian Pacific Journal of Tropical Disease 4, 120-124.
10
Khanjari, A., Partovi, R., Abbaszadeh, S., Nemati, G., Bahonar, A., Misaghi, A., Akhondzadeh-Basti, A., Alizadeh-Ilanjegh, A., Motaghifar, A., 2010. A retrospective survey of fasciolosis and dicrocoeliosis in slaughtered animals in Meisam abattoir, Tehran, Iran (2005-2008). Vet Res Forum 1, 174-178.
11
Mas-Coma, M.S., Esteban, J.G., Bargues, M.D., 1999. Epidemiology of human fascioliasis: a review and proposed new classification. Bull World Health Organ 77, 340-346.
12
Mehrabani, D., Oryan, A., Sadjjadi, S.M., 1999. Prevalence of Echinococcus granulosus infection in stray dogs and herbivores in Shiraz, Iran. Veterinary Parasitology 86, 217-220.
13
Oryan, A., Goorgipour, S., Moazeni, M., Shirian, S., 2012. Abattoir prevalence, organ distribution, public health and economic importance of major metacestodes in sheep, goats and cattle in Fars, southern Iran. Trop Biomed 29, 349-359.
14
Robinson, M.W., Dalton, J.P., 2009a. Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases. Philos Trans R Soc Lond B Biol Sci 364, 2763-2776.
15
Robinson, M.W., Dalton, J.P., 2009b. Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases. Philosophical Transactions of the Royal Society B: Biological Sciences 364, 2763-2776.
16
Rokni, M., 2009. Echinococcosis/hydatidosis in Iran. Iran J Parasitol 4, 1-16.
17
Rokni, M.B., 2008. The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol 102, 283-295.
18
Rostami Nejad, M., Jahani-Sherafat, S., Cheraghipour, K., Nazemallhoseini Mojarad, E., Taghipour, N., Zali, M., 2012. Hydatic cyst prevalence in slaughtered animals, A neglected health problem. J Paramed Sci 3, 25-29.
19
Sadjjadi, S.M., 2006. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int 55 Suppl, S197-202.
20
Soufi, M., 2004. Morpho-climatic classification of gullies in Fars province, south west of IR Iran. 3th International Soil Conservation Organisation Conference, Brisbane.
21
Tappe, K.H., Mousavi, S.J., Barazesh, A., 2011. Prevalence and fertility of hydatid cyst in slaughtered livestock of Urmia city, Northwest Iran. J Parasitol Vector Biol 3, 29-32.
22
World Health Organization, 2007. Report of the WHO Informal Meeting on use of triclabendazole in fascioliasis control. WHO Headquarters. Geneva, Switzerland.
23
Ziaei, H., Fakhar, M., Armat, S., 2011. Epidemiological aspects of cystic echinococcosis in slaughtered herbivores in Sari abattoir, North of Iran. J Parasit Dis 35, 215-218.
24
ORIGINAL_ARTICLE
Identification of ectoparasites in indigenous poultry in southern areas of West Azerbaijan, Iran: A study on the prevalence and importance of these parasites
Poultry products are considered as one of the most important sources of food for humans, worldwide. Indigenous poultry production has grown in popularity among villagers and some urbanites due to simple maintenance conditions, adequate adaption of poultry to different climatic conditions, and acceptable product yield. Parasites are among the main pathogenic agents, threatening the health of poultry and poultry products. The present study was carried out in northwest of Iran between March 2013 and December 2015 to determine the prevalence of ectoparasite species, infesting local chickens. Different parts of the bird''s body were inspected for ectoparasites. The parasites were collected from the birds by displaying the feathers horizontally against the anatomical orientation for the purpose of exposure. Then, the separated parasites were identified according to the diagnostic guidelines. Based on the findings, of 160 chickens examined, 110 (68.7%) samples had one or more types of ectoparasites. The prevalence of infestation was higher in females (74.5%) than males (56%), although the difference was not statistically significant (P>0.05). Menopon gallinae was the most frequent species (65.4%), followed by Menacanthus stramineus (37.2%), Lipeurus caponis (10.9%), and Dermanyssus gallinae (9.1%). The present study indicated that ectoparasitic infestation was highly prevalent among chickens in the studied areas. However, further detailed studies are recommended with a focus on ectoparasites infestations and their impacts.
https://archrazi.areeo.ac.ir/article_107510_bd3a1ace7e36face6b8ead48e6bdacb3.pdf
2016-12-01
253
258
10.22034/ari.2016.107510
Ectoparasite
Local chickens
Iran
M.
Ebrahimi
mansour.ebrahimi91@yahoo.com
1
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz,Iran
LEAD_AUTHOR
K.
Samiei
2
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
D.
Anousheh
3
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
M.H.
Razi Jalali
4
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz,Iran
AUTHOR
Bhat, S.A., Wani, M.Y., Khojuria, J.K., Katoch, R., Dhama, K., 2014. A Rare Report of Ectoparasites in Backyard Poultry in Jammu Region: Prevalence Study and Economic Importance. Asian J Anim Vet Adv 9, 727-731.
1
Biu, A.A., Agbede, R.I., Peace, P., 2007. Studies on ectoparasites of poultry in Maiduguri, Nigeria. Nigerian J Parasitol 28, 69-72.
2
Buriro, S.N., Akbar, S.S., 1978. Incidence and occurrence of ectoparasites of poultry in Pakistan. Zeitschrift für Angewandte Entomologie 86, 1-8.
3
Chirico, J., Eriksson, H., Fossum, O., Jansson, D., 2003. The poultry red mite, Dermanyssus gallinae, a potential vector of Erysipelothrix rhusiopathiae causing erysipelas in hens. Medical and Veterinary Entomology 17, 232-234.
4
Colebrook, E., Wall, R., 2004. Ectoparasites of livestock in Europe and the Mediterranean region. Veterinary Parasitology 120, 251-274.
5
Ebrahimi, M., Ahmadi, A., Mohammadpour, H., 2013. Survey on infestation to ectoparasites in native poultry of Najaf-Abad. J Food Microbiol 4, 17-20.
6
Eslami, A., Ghaemi, P., Rahbari, S., 2009. Parasitic Infections of Free –Chickens from Golestan Province Iran. Iran J Parasitol 4, 10-14.
7
Hashemzade-farhang, H., Namdarian, M.R., Shirazi, S., Shahbazi, P., 2008. Survey of ectoparasites in native poultry of Tabriz city, Iran. Iran Vet J 4, 97-100.
8
Kansal, G., Singh, H.S., 2014. Incidence of Ectoparasites in Broiler Chicken in Meerut. IOSR J Agri Vet Sci 7, Singh, H. S. (). . 7: .
9
Mungube, E.O., Bauni, S.M., Tenhagen, B.-A., Wamae, L.W., Nzioka, S.M., Muhammed, L., Nginyi, J.M., 2008. Prevalence of parasites of the local scavenging chickens in a selected semi-arid zone of Eastern Kenya. Tropical Animal Health and Production 40, 101-109.
10
Nnadi, P.A., George, S.O., 2010. A Cross-Sectional Survey on Parasites of Chickens in Selected Villages in the Subhumid Zones of South-Eastern Nigeria. Journal of Parasitology Research 2010.
11
Permin, A., Esmann, J.B., Hoj, C.H., Hove, T., Mukaratirwa, S., 2002. Ecto-, endo- and haemoparasites in free-range chickens in the Goromonzi District in Zimbabwe. Preventive Veterinary Medicine 54, 213-224.
12
Phiri, I.K., Phiri, A.M., Ziela, M., Chota, A., Masuku, M., Monrad, J., 2007. Prevalence and distribution of gastrointestinal helminths and their effects on weight gain in free-range chickens in Central Zambia. Tropical Animal Health and Production 39, 309-315.
13
Radfar, M.H., Khedri, J., Adinehbeigi, K., Nabavi, R., Rahmani, K., 2012. Prevalence of parasites and associated risk factors in domestic pigeons (Columba livia domestica) and free-range backyard chickens of Sistan region, east of Iran. Journal of Parasitic Diseases 36, 220-225.
14
Ruff, M.D., 1999. Important parasites in poultry production systems. Veterinary Parasitology 84, 337-347.
15
Sparagano, O.A.E., George, D.R., Harrington, D.W.J., Giangaspero, A., 2014. Significance and Control of the Poultry Red Mite, Dermanyssus gallinae. Annual Review of Entomology 59, 447-466.
16
Sychra, O., Harmat, P., Literák, I., 2008. Chewing lice (Phthiraptera) on chickens (Gallus gallus) from small backyard flocks in the eastern part of the Czech Republic. Veterinary Parasitology 152, 344-348.
17
Tamiru, F., Dagmawit, A., Askale, G., Solomon, S., Morka, D., Waktole, T., 2014. Prevalence of Ectoparasite Infestation in Chicken in and Around Ambo Town, Ethiopia. Vet Sci Technol.
18
Tolossa, Y., Shafi, Z., Basu, A., 2009. Ectoparasites and gastrointestinal helminths of chickens of three agro-climatic zones in Oromia Region, Ethiopia. Animal Biology 59, 289-297.
19
Van De Weerd, H.A., Keatinge, R., Roderick, S., 2009. A review of key health-related welfare issues in organic poultry production. World''s Poultry Science Journal 65, 649-684.
20
Wall, R., Shearer, D., 2012. Veterinary Entomology: Arthropod Ectoparasites of Veterinary Importance, Springer Netherlands
21
ORIGINAL_ARTICLE
Histopathological changes and biochemical analysis of sulfadiazine injected in egg in chicken embryo pectoral muscles
This study aimed to investigate the effects of different doses of sulfadiazine on embryonic chicken pectoral muscles. In total, 100 fertile eggs were obtained and divided into five groups of control (no injection) and sulfadiazine injection at doses of 2, 10, 30, and 70 mg/kg. After hatching, pectoral muscle tissues were harvested from the newly hatched chickens for histopathological examination and measurement of oxidative stress parameters. Microscopic examination of pectoral muscle samples indicated that sulfadiazine administration changed the histopathological structure of chicken pectoral muscles only at very high doses (30 and 70 mg/kg). Major histopathologic events associated with sulfadiazine cytotoxicity were multifocal degeneration, necrotic tissue changes, and inflammatory cell infiltration (predominantly mononuclear cells) around degenerated and necrotic muscle fibers. Moreover, sulfadiazine at doses of 10, 30 and 70 mg/kg increased malondialdehyde level and decreased glutathione, ferric reducing antioxidant power, and total carotenoid, which indicated oxidative damage in broiler skeletal muscles. Therefore, it could be concluded that in-egg administration of up to 10 mg/kg of sulfadiazine is safe for chicken embryo, whereas dosage of 30 mg/kg (or above) is considered highly toxic.
https://archrazi.areeo.ac.ir/article_107511_fa11b91dd25645d7a36b2e49c836ad18.pdf
2016-12-01
259
267
10.22034/ari.2016.107511
Sulfadiazine toxicity
Pectoral muscle
Chicken embryo
H.
Golshahi
1
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran,Iran
AUTHOR
R.
Sayrafi
2
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
A.
Araghi
a.araghi@ausmt.ac.ir
3
Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
LEAD_AUTHOR
M.
Abouhosseini Tabari
4
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Accinelli, C., Koskinen, W.C., Becker, J.M., Sadowsky, M.J., 2007. Environmental fate of two sulfonamide antimicrobial agents in soil. J Agric Food Chem 55, 2677-2682.
1
Archile-Contreras, A.C., Purslow, P.P., 2011. Oxidative stress may affect meat quality by interfering with collagen turnover by muscle fibroblasts. Food Research International 44, 582-588.
2
Baran, W., Sochacka, J., Wardas, W., 2006. Toxicity and biodegradability of sulfonamides and products of their photocatalytic degradation in aqueous solutions. Chemosphere 65, 1295-1299.
3
Barragry, T.B., 1994. Aminoglycosides, macrolides, and lincosamides. In: Veterinary Drug Therapy, Williams & Wilkins, Philadelphia, USA.
4
Bass, A.D., Yntema, C.L., Hammond, W.S., Frazer, M.L., 1951. Studies on the mechanism by which sulfadiazine affects the survival of the mammalian embryo. J Pharmacol Exp Ther 101, 362-367.
5
Booth, N.H., 1973. Development of a regulatory research program in Veterinary medical toxicology. J Vet Toxicol 15, 100-100.
6
Casella, I.G., Contursi, M., Gioia, D., 2012. Development of a Liquid Chromatography/Amperometric Detection Method for the Determination of Multiresidue Sulfonamide Antibiotics in Meat-Based Baby Foods. Electroanalysis 24, 2125-2133.
7
Cheong, C.K., Hajeb, P., Jinap, S., Ismail-Fitry, M.R., 2010. Sulfonamides determination in chicken meat products from Malaysia. Int Food Res J 17, 885-892.
8
Collins, B.K., Moore, C.P., Hagee, J.H., 1986. Sulfonamide-associated keratoconjunctivitis sicca and corneal ulceration in a dysuric dog. J Am Vet Med Assoc 189, 924-926.
9
Czauderna, M., Kowalczyk, J., Marounek, M., 2011. The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci 879, 2251-2258.
10
Daft, B.M., Bickford, A.A., Hammarlund, M.A., 1989. Experimental and field sulfaquinoxaline toxicosis in Leghorn chickens. Avian Dis 33, 30-34.
11
Del Rio, D., Stewart, A.J., Pellegrini, N., 2005. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 15, 316-328.
12
Devasagayam, T.P., Tilak, J.C., Boloor, K.K., Sane, K.S., Ghaskadbi, S.S., Lele, R.D., 2004. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52, 794-804.
13
Fischer, L.J., Thulin, A.J., Zabik, M.E., Booren, A.M., Poppenga, R.H., Chapman, K.J., 1992. Sulfamethazine and its metabolites in pork: effects of cooking and gastrointestinal absorption of residues. Journal of Agricultural and Food Chemistry 40, 1677-1682.
14
Gibson, X.A., Shartava, A., McIntyre, J., Monteiro, C.A., Zhang, Y., Shah, A., Campbell, N.F., Goodman, S.R., 1998. The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro. Blood 91, 4373-4378.
15
Guzik, T.J., West, N.E., Pillai, R., Taggart, D.P., Channon, K.M., 2002. Nitric oxide modulates superoxide release and peroxynitrite formation in human blood vessels. Hypertension 39, 1088-1094.
16
Halliwell, B., Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. The American Journal of Medicine 91, S14-S22.
17
Islam, M.K., Akter, S., Bala, S., Z., H.M., Akter, M.S., 2012. Investigation on the counteracting effect of spirulina against potentated sulfonamides (COTRIM DS) side effects in rat. Bangladesh J Vet Med 10, 81-86.
18
Kato, T., Kitagawa, S., 1974. Effects of a new antibacterial sulfonamide (CS-61) on mouse and rat fetuses. Toxicology and Applied Pharmacology 27, 20-27.
19
Lebkowska-Wieruszewska, B.I., Kowalski, C.J., 2010. Sulfachlorpyrazine residues depletion in turkey edible tissues. J Vet Pharmacol Ther 33, 389-395.
20
Lee, C.Y., Lee, B.D., Na, J.C., An, G., 2010. Carotenoid Accumulation and Their Antioxidant Activity in Spent Laying Hens as Affected by Polarity and Feeding Period. Asian Australas. J. Anim. Sci 23, 799-805.
21
Liman, B.C., Kanbur, M., Eraslan, G., Baydan, E., Dinç, E., Karabacak, M., 2015. Effects of various freezing and cooking processes on the residues of sulfamethazine in broiler tissues. Ankara Uni Vet Fakültesi Dergisi 62, 6-13.
22
Littlefield, N.A., Sheldon, W.G., Allen, R., Gaylor, D.W., 1990. Chronic toxicity/carcinogenicity studies of sulphamethazine in Fischer 344/N rats: Two-generation exposure. Food and Chemical Toxicology 28, 157-167.
23
Liu, J., Fang, G., Zhang, Y., Zheng, W., Wang, S., 2009. Development of a chemiluminescent enzyme-linked immunosorbent assay for five sulfonamide residues in chicken muscle and pig muscle. Journal of the Science of Food and Agriculture 89, 80-87.
24
Mahmoudi, R., Gajarbeygi, P., Norian, R., Farhoudi, K., 2014. Choramphenicol, Sulfonamide and Tetracycline residues in cultured Rainbow trout meat (Oncorhynchus mykiss). Bulgarian J Vet Med 17, 147-152.
25
Malik, H.E.E., Omer, J.E., Elamin, K.M., 2013. Effect of Sulfonamides Residues on Egg Quality Traits. Int J Poultry Sci 12, 312-317.
26
Møller, A.P., Biard, C., Blount, J.D., Houston, D.C., Ninni, P., Saino, N., Surai, P.F., 2000. Carotenoid-dependent signals: indicators of foraging efficiency, immunocompetence or detoxification ability? . Avian Poultry Biol Rev 11, 137-159.
27
Nordberg, J., Arnér, E.S.J., 2001. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system1. Free Radical Biology and Medicine 31, 1287-1312.
28
Paget, G.E., Thorpe, E., 1964. A TERATOGENIC EFFECT OF A SULPHONAMIDE IN EXPERIMENTAL ANIMALS. British Journal of Pharmacology and Chemotherapy 23, 305-312.
29
Poirier, L.A., Doerge, D.R., Gaylor, D.W., Miller, M.A., Lorentzen, R.J., Casciano, D.A., Kadlubar, F.F., Schwetz, B.A., 1999. An FDA review of sulfamethazine toxicity. Regul Toxicol Pharmacol 30, 217-222.
30
Prescott, J.F., 2000. Antimicrobials therapy in veterinary medicine, Blackwell, Ames, Iowa, USA.
31
Sadighara, P., Jahed Khaniki, G., Baseri, E., Dehghani, M.H., Barin, A., Mazaheri Nezhad Fard, R., 2013. Effect of Bisphenol A on the Quality Characteristics of Meat in a Chicken Embryo Model. Sci Int 1, 375-378.
32
Savary-Auzeloux, I., Durand, D., Gruffat, D., Bauchart, D., Ortigues-Marty, I., 2008. Food restriction and refeeding in lambs influence muscle antioxidant status. Animal 2, 738-745.
33
Sayrafi, R., Golshahi, H., Araghi, A., Seifi, S., 2015. Histopathological evaluation of dose dependent sulfadiazine-associated nephrotoxicity and alteration on oxidative stress in chicken embryos. Zahedan J Res Med Sci in press.
34
Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L., Hawkins Byrne, D., 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis 19, 669-675.
35
Wang, H., Xu, Y., Song, W., Zhao, Q., Zhang, X., Zeng, Q., Chen, H., Ding, L., Ren, N., 2011. Automatic sample preparation of sulfonamide antibiotic residues in chicken breast muscle by using dynamic microwave-assisted extraction coupled with solid-phase extraction. J Sep Sci 34, 2489-2497.
36
Zhang, W., Xiao, S., Ahn, D.U., 2013. Protein oxidation: basic principles and implications for meat quality. Crit Rev Food Sci Nutr 53, 1191-1201.
37
ORIGINAL_ARTICLE
The effect of equine chorionic gonadotrophin (ecg) injection combined with prostaglandin F2α (pGF2α) and gonadotrophin releasing hormone (GnRH) treatment on reproductive performance of Zandi ewes during non-breeding season
In this study, we aimed to investigate reproductive performance in estrus-induced Zandi ewes treated with equine chorionic gonadotrophin (eCG) injection in combination with prostaglandin F2α (PGF2α)and gonadotrophin releasing hormone (GnRH) during non-breeding season. The estrous cycle was synchronized using controlled internal drug release (CIDR) for 12 days. The ewes were randomly assigned to five groups (n=100 in each group). The first group (control) did not receive hormone injection, the second group (eCG) received 400 IU of eCG at the time of CIDR removal, the third group (eCG+PGF2α) received 400 IU of eCG and 1 ml of PGF2α at the time of CIDR removal, the fourth group (eCG+GnRH) received 400 IU of eCG at the time of CIDR removal and 1 ml of GnRH injection on the day of insemination, and the fifth group (eCG+PGF2α+GnRH) received 400 IU of eCG, 1 ml of PGF2α at the time of CIDR removal, and 1 ml of GnRH on the day of insemination. The results showed that the heat response rate was higher in all the groups receiving hormonal injections compared with the control group (P<0.05). In addition, the lambing and parturition rateswere significantly (P<0.05) higher in the groups receiving GnRH compared with other treatments.In fact, in groups receiving eCG and PGF2α, lambing and parturition rateswere higher than the control group (P>0.05). In conclusion, use of eCG at the time of CIDR removal and GnRH on the day of insemination increased reproductive performance. Therefore, it can be applied as a strategy for increasing lambing rate in ewes.
https://archrazi.areeo.ac.ir/article_107512_ae58f731b8ff494b52b72efedddaa79f.pdf
2016-12-01
269
279
10.22034/ari.2016.107512
Lambing rate
eCG
GnRH
PGF2α
Zandi ewes
A.
Hosseinzadeh Aski
1
Department of Animal Science, Faculty of Veterinary, Islamic Azad University, Ghaemshahr Branch, Ghaemshahr, Iran
AUTHOR
R.
Masoudi
rezamasoudi@ut.ac.ir
2
Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
LEAD_AUTHOR
A.
Zare Shahneh
3
Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
AUTHOR
S.
Asadzadeh
4
Department of Animal Science, Faculty of Veterinary, Islamic Azad University, Ghaemshahr Branch, Ghaemshahr, Iran
AUTHOR
E.
Dirandeh
5
Department of Animal Science, Faculty of Agriculture, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
AUTHOR
H.
Sadeghipanah
6
Animal Science Research Institute, Karaj, Iran
AUTHOR
Abdalla, E.B., Farrag, B., Hashem, A.L.S., Khalil, F.A., Abdel-Fattah, M.S., 2014. Effect of progestagen, PGF2α, PMSG and GnRH on estrus synchronization and some reproductive and productive traits in Barki ewes. J Agroaliment Proc Technol 20, 93-101.
1
Abdel-Megeed, I.I., 2006. Economic evaluation of lamb production in Rahmani sheep under two regimes for super-ovulation. Faculty of Agriculture. Cairo University.
2
Abdullah, A.Y., Husein, M.Q., Kridli, R.T., 2002. Protocols for estrus synchronization in Awassi ewes under arid environmental conditions. Asian-Australian J Anim Sci 15, 957-962.
3
Ali, A., 2007. Effect of time of eCG administration on follicular response and reproductive performance of FGA-treated Ossimi ewes. Small Rumin Res 72, 33-37.
4
Ataman, M.B., Aköz, M., 2006. GnRH-PGF2α and PGF2α-PGF2α synchronization in Akkaraman cross-bred sheep in the breeding season. Bulletin Vet Inst Pulawy 50, 101-104.
5
Azawi, O.I., Al-Mola, M.K., 2011. A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation. Trop Anim Health Prod 43, 1351-1355.
6
Baird, D.T., McNeilly, A.S., 1981. Gonadotrophin control of follicular development and function during the estrous cycle of the ewe. J Reprod Fertil 30, 119-133.
7
Bartlewski, P.M., Duggavathi, R., Aravindakshan, J., Barrett, D.M., Cook, S.J., Rawlings, N.C., 2003. Effects of a 6-day treatment with medroxyprogesterone acetate after prostaglandin F2 alpha-induced luteolysis at midcycle on antral follicular development and ovulation rate in nonprolific Western white-faced ewes. Biol Reprod 68, 1403-1412.
8
Beck, N.F.G., Jones, M., Davies, B., Peters, A.R., Williams, S.P., 1996. Oestrus synchronization in ewes: the effect of combining a prostaglandin analogue with a GnRH agonist (buserelin). Animal Science 62, 85-87.
9
Bister, J.L., Noël, B., Perrad, B., Mandiki, S.N.M., Mbayahaga, J., Paquay, R., Control of ovarian follicles activity in the ewe. Domestic Animal Endocrinology 17, 315-328.
10
Boscos, C.M., Samartzi, F.C., Dellis, S., Rogge, A., Stefanakis, A., Krambovitis, E., Use of progestagen 2013;gonadotrophin treatments in estrus synchronization of sheep. Theriogenology 58, 1261-1272.
11
Cline, M.A., Ralston, J.N., Seals, R.C., Lewis, G.S., 2001. Intervals from norgestomet withdrawal and injection of equine chorionic gonadotropin or P.G. 600 to estrus and ovulation in ewes. J Anim Sci 79, 589-594.
12
Cognié, Y., Baril, G., Poulin, N., Mermillod, P., 2003. Current status of embryo technologies in sheep and goat. Theriogenology 59, 171-188.
13
Dadashpour Davachi, N., Kohram, H., Zeinoaldini, S., 2011. Effect of the presence of corpus luteum on the ovary and the new oocyte recovery method on the oocyte recovery rate and meiotic competence of ovine oocytes. Afr J Biotechnol 10, 9706-9709.
14
Dadashpour Davachi, N., Zare Shahneh, A., Kohram, H., Zhandi, M., Dashti, S., Shamsi, H., Moghadam, R., 2014. In vitro ovine embryo production: the study of seasonal and oocyte recovery method effects. Iran Red Crescent Med J 16, e20749.
15
Dirandeh, E., Kohram, H., Zare Shahne, A., 2009. GnRH injection before artificial insemination (AI) alters follicle dynamics in Iranian Holstein cows. African J Biotechnol 8, 3672-3676.
16
Evans, G., 1988. Current topics in artificial insemination of sheep. Aust J Biol Sci 41, 103-116.
17
Fitzgerald, J.A., Ruggles, A.J., Stellflug, J.N., Hansel, W., 1985. A Seven-Day Synchronization Method for Ewes Using Medroxyprogesterone Acetate (MAP) and Prostaglandin F2α. Journal of Animal Science 61, 466-469.
18
Fuerst, K.J., Bartlewski, P.M., King, W.A., 2009. Relationship between circulating concentrations of ovarian steroids and the superovulatory responses in anestrous ewes following a multiple-dose pFSH regimen. Small Rumin Res 82, 144-148.
19
Gonzalez-Bulnes, A., Garcia-Garcia, R.M., Santiago-Moreno, J., Dominguez, V., Lopez-Sebastian, A., Cocero, M.J., 2003. Reproductive season affects inhibitory effects from large follicles on the response to superovulatory FSH treatments in ewes. Theriogenology 60, 281-288.
20
Gordon, I., 1975. The use of progestagens in sheep bred by natural and artificial insemination. Ann. Biol. anim. Bioch. Biophys. 15, 303-315.
21
Hay, M.F., Moor, R.M., 1975. Functional and structural relationships in the Graafian follicle population of the sheep ovary. J Reprod Fertil 45, 583-593.
22
Herbert, C.A., Trigg, T.E., 2005. Applications of GnRH in the control and management of fertility in female animals. Anim Reprod Sci 88, 141-153.
23
Husein, M.Q., Kridli, R.T., 2002. Reproductive Responses of Awassi Ewes Treated with either Naturally Occurring Progesterone or Synthetic Progestagen. Asian Australas. J. Anim. Sci 15, 1257-1262.
24
Husein, T., Hamit, Y., 2005. Synchronization of estrous in Hamdani ewes: the use of different PMSG doses. Bulletin Vet Inst Pulawy 49, 311-314.
25
Keisler, D.H., Keisler, L.W., 1989. Formation and function of GnRH-induced subnormal corpora lutea in cyclic ewes. J Reprod Fertil 87, 265-273.
26
Kermani Moakhar, H., Kohram, H., Zareh Shahneh, A., Saberifar, T., 2012. Ovarian response and pregnancy rate following different doses of eCG treatment in Chall ewes. Small Rumin Res 102, 63-67.
27
Kruip, T.A.M., Brand, A., 1975. Follicular growth during the normal cycle and after treatment with progestagens in the ewe. Ann Biol Anim Bioch Biophys 15, 191-204.
28
Leyva, V., Buckrell, B.C., Walton, J.S., 1998. Regulation of follicular activity and ovulation in ewes by exogenous progestagen. Theriogenology 50, 395-416.
29
Luther, J.S., Grazul-Bilska, A.T., Kirsch, J.D., Weigl, R.M., Kraft, K.C., Navanukraw, C., Pant, D., Reynolds, L.P., Redmer, D.A., 2007. The effect of GnRH, eCG and progestin type on estrous synchronization following laparoscopic AI in ewes. Small Rumin Res 72, 227-231.
30
Mandiki, S. N. M., Noël, B., Bister, J.L., Peeters, R., Beerlandt, G., Decuypere, E., Visscher, A., Sueß, R., Kaulfuß, K.H., Paquay, R., 2000. Pre-ovulatory follicular characteristics and ovulation rates in different breed crosses, carriers or non-carriers of the Booroola or Cambridge fecundity gene. Anim Reprod Sci 63, 77-88.
31
Masoudi, R., Kohram, H., Shahne, A. Z., Davoud, S. D. M. A., 2012a. Effect of estradiol and oxytocin on ovine cervical relaxation. Af J Biotech 11, 2803-2806.
32
Masoudi, R., Kohram, H., Shahne, A. Z., Davoud, S. D. M. A., Sharif, A. A., 2012b. Effects of exogenous oxytocin on cervical penetration of Iranian ewes. Af J Biotech 11, 2398-2401.
33
Masoudi, R., Sharafi, M., Zareh Shahneh, A., Towhidi, A., Kohram, H., Esmaeili, V., Shahverdi, A., Davachi, N.D., 2016. Fertility and flow cytometry study of frozen-thawed sperm in cryopreservation medium supplemented with soybean lecithin. Cryobiology 73, 69-72.
34
McNatty, K.P., Heath, D.A., Hudson, N., Clarke, I.J., 1990. Effect of long-term hypophysectomy on ovarian follicle populations and gonadotrophin-induced adenosine cyclic 3',5'-monophosphate output by follicles from Booroola ewes with or without the F gene. J Reprod Fertil 90, 515-522.
35
Menchaca, A., Rubianes, E., 2004. New treatments associated with timed artificial insemination in small ruminants. Reprod Fertil Dev 16, 403-413.
36
Menchaca, A., Vilarino, M., Pinczak, A., Kmaid, S., Saldana, J.M., 2009. Progesterone treatment, FSH plus eCG, GnRH administration, and Day 0 Protocol for MOET programs in sheep. Theriogenology 72, 477-483.
37
Mhim, M., Curran, N., Hyttel, P., Boland, M.P., Roche, J.F., 1999. Resumption of meiosis in cattle oocytes from preovulatory follicles with a short and a long duration of dominance. J Reprod Fertil 116, 293-304.
38
Moradi Kor, N., Mohammadi Khanghah, K., Veisi, A., 2012. Efficiency of short time protocols based on combined FGA, PGF2α, GnRH and eCG treatments on oestrus synchronization and reproductive performance of kermani ewes during the breeding season. Int J Biol Med Res 3, 1966-1970.
39
Mossa, F., Duffy, P., Naitana, S., Lonergan, P., Evans, A.C., 2007. Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes. Anim Reprod Sci 100, 391-396.
40
Naqvi, S.M., Gulyani, R., 1998. The effect of gonadotrophin releasing hormone and follicle stimulating hormone in conjunction with pregnant mare serum gonadotrophin on the superovulatory response in crossbred sheep in India. Trop Anim Health Prod 30, 369-376.
41
Noël, B., Bister, J.L., Pierquin, B., Paquay, R., Effects of FGA and PMSG on follicular growth and LH secretion in Suffolk ewes. Theriogenology 41, 719-727.
42
Pawson, A.J., McNeilly, A.S., 2005. The pituitary effects of GnRH. Anim Reprod Sci 88, 75-94.
43
Rubianes, E., de Castro, T., Kmaid, S., 1998. Estrous response after a short progesterone priming in seasonally anestrous goats. Theriogenology 49, 356.
44
Rubianes, E., Ibarra, D., Ungerfeld, R., Carbajal, B., de Castro, T., 1995. Superovulatory response in anestrous ewes is affected by the presence of a large follicle. Theriogenology 43, 465-472.
45
Safdarian, M., Kafi, M., Hashemi, M., 2006. Reproductive performance of Karakul ewes following different oestrous synchronisation treatments outside the natural breeding season. S Afr J Anim Sci 36, 229-234.
46
Sirjani, M.A., Kohram, H., Shahir, M.H., 2012. Effects of eCG injection combined with FSH and GnRH treatment on the lambing rate in synchronized Afshari ewes. Small Rumin Res 106, 59-63.
47
Titi, H.H., Kridli, R.T., Alnimer, M.A., 2010. Estrus synchronization in sheep and goats using combinations of GnRH, progestagen and prostaglandin F2alpha. Reprod Domest Anim 45, 594-599.
48
Turk, G., Gur, S., Sonmez, M., Bozkurt, T., Aksu, E.H., Aksoy, H., 2008. Effect of exogenous GnRH at the time of artificial insemination on reproductive performance of Awassi ewes synchronized with progestagen-PMSG-PGF2alpha combination. Reprod Domest Anim 43, 308-313.
49
Zarkawi, M., 2001. Oestrous synchronisation and twinning rate of Syrian Awassi ewes treated with progestagen and PMSG during the breeding season. New Zealand Journal of Agricultural Research 44, 159-163.
50
Zeleke, M., Greyling, J.P.C., Schwalbach, L.M.J., Muller, T., Erasmus, J.A., 2010. Effect of progestagen and PMSG on oestrous synchronization and fertility in Dorper ewes during the transition period. Small Rumin Res 56, 47-53.
51
Zonturlu, A., Aral, F., Ozyurtlu, N., Yavuzer, U., 2008. Synchronization of estrus using FGA and CIDR intravaginal pessaries during the transition period in awassi ewes. J Anim Vet Adv 7, 1093-1096.
52
ORIGINAL_ARTICLE
Alimentary tract parasites of vervet monkeys (Cercopithecus aethiops): A potential reservoir for human transmission
Monkeys are important experimental models for investigating human diseases. The aim of this study was to survey the alimentary tract parasites among imported vervet monkeys (Cercopithecus aethiops) to Iran. Fecal samples were collected from 40 vervets imported from Tanzania to Razi Vaccine and Serum Research Institute of Iran. Fecal samples were assessed by direct smear and Telman sedimentation methods. The results of microscopic examination demonstrated that all (100%) the animals were infected with different alimentary tract parasites. The protozoan parasites comprised of Iodamoeba butschlii (85%), Entamoeba coli (72.5%), Entamoeba histolytica/dispar (37.5%), Chilomastix mesnili (12.5%), Balantidium coli (10%), Blastocystis hominis (7.5%), and Giardia intestinalis (5%). Additionally, eggs of some helminths, including Physaloptera caucasica (27.5%), Trichostrongylus spp. (7.5%), Trichuris trichiura (7.5%), Bertiella spp. (2.5%), and Strongyloides fulleborni (2.5%), were detected. The presence of gastrointestinal parasites in vervetsposes a risk for human or experimental results. Thus, the diagnosis and treatment of these parasites should be considered before any laboratory assay.
https://archrazi.areeo.ac.ir/article_107513_4e964e8426b9039c9c52845d4e79899d.pdf
2016-12-01
277
281
10.22034/ari.2016.107513
Vervet monkeys
Alimentary tract
PARASITES
Telman
A.
Dalimi
dalimi4@yahoo.com
1
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
LEAD_AUTHOR
G.
Motamedi
2
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
AUTHOR
M.H.
Hablolvarid
3
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
AUTHOR
A.
Abdoli
4
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
AUTHOR
Baker, D.G., 1998. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clin Microbiol Rev 11, 231-266.
1
Berrilli, F., Prisco, C., Friedrich, K.G., Di Cerbo, P., Di Cave, D., De Liberato, C., 2011. Giardia duodenalis assemblages and Entamoeba species infecting non-human primates in an Italian zoological garden: zoonotic potential and management traits. Parasit Vectors 4, 199.
2
Bezjian, M., Gillespie, T.R., Chapman, C.A., Greiner, E.C., 2008. Coprologic evidence of gastrointestinal helminths of forest baboons, Papio anubis, in Kibale National Park, Uganda. J Wildl Dis 44, 878-887.
3
Carvalho Filho, P.R.D., Cardozo, S.V., Ribeiro, C.T., Md., M.S., G., L.C.W., 2006. Intestinal protozoa in apprehended New World nonhuman primates. Braz J vet Res anim Sci 43, 354-361.
4
DuPont, H.L., 2009. Systematic review: the epidemiology and clinical features of travellers' diarrhoea. Aliment Pharmacol Ther 30, 187-196.
5
Furtado, A.P., Batista, E.d.J.O., Gonçalves, E.C., Silva, A.M.H.O., Melo, F.T.V., Giese, E.G., Santos, J.N., 2012. Human Bertielliasis in Amazonia: Case Report and Challenging Diagnosis. PLoS Negl Trop Dis 6, e1580.
6
Herodin, F., Thullier, P., Garin, D., Drouet, M., 2005. Nonhuman primates are relevant models for research in hematology, immunology and virology. Eur Cytokine Netw 16, 104-116.
7
Herwaldt, B.L., 2001. Laboratory-acquired parasitic infections from accidental exposures. Clin Microbiol Rev 14, 659-688, table of contents.
8
Howells, M.E., Pruetz, J., Gillespie, T.R., 2011. Patterns of gastro-intestinal parasites and commensals as an index of population and ecosystem health: the case of sympatric western chimpanzees (Pan troglodytes verus) and guinea baboons (Papio hamadryas papio) at Fongoli, Senegal. Am J Primatol 73, 173-179.
9
Kooriyama, T., Hasegawa, H., Shimozuru, M., Tsubota, T., Nishida, T., Iwaki, T., 2012. Parasitology of five primates in Mahale Mountains National Park, Tanzania. Primates 53, 365-375.
10
Kooriyama, T., Inaba, A., Nishida, T., Iwaki, T., 2010. Case report of helminths and lung mite infection in the red-tailed monkey, Cercopithecus ascanius schmidti, in Mahale Mountains National Park, Tanzania. Primates 51, 183-188.
11
Lee, J.I., Kim, N.A., Ahn, K.H., Park, C.G., 2010. Investigation of helminths and protozoans infecting old world monkeys: captive vervet, cynomolgus, and rhesus monkeys. Korean J Vet Res 50, 273-277.
12
Legesse, M., Erko, B., 2004. Zoonotic intestinal parasites in Papio anubis (baboon) and Cercopithecus aethiops (vervet) from four localities in Ethiopia. Acta Trop 90, 231-236.
13
Malik, S., Srivastava, V.K., Samantaray, J.C., 2013. Human bertiellosis from north India. Indian J Pediatr 80, 258-260.
14
McCarthy, J., Moore, T.A., 2000. Emerging helminth zoonoses. International Journal for Parasitology 30, 1351-1359.
15
McSorley, H.J., Maizels, R.M., 2012. Helminth infections and host immune regulation. Clin Microbiol Rev 25, 585-608.
16
Munene, E., Otsyula, M., Mbaabu, D.A.N., Mutahi, W.T., Muriuki, S.M.K., Muchemi, G.M., 1998. Helminth and protozoan gastrointestinal tract parasites in captive and wild-trapped African non-human primates. Veterinary Parasitology 78, 195-201.
17
Muriuki, S.M.K., Murugu, R.K., Munene, E., Karere, G.M., Chai, D.C., 1998. Some gastro-intestinal parasites of zoonotic (public health) importance commonly observed in old world non-human primates in Kenya. Acta Tropica 71, 73-82.
18
Parmar, S., Jani, R., Mathakiya, R., 2012. Study of parasitic infections in non-human primates of Gujarat state, India. Vet World 5, 362-364.
19
Parr, N.A., Fedigan, L.M., Kutz, S.J., 2013. A coprological survey of parasites in white-faced capuchins (Cebus capucinus) from Sector Santa Rosa, ACG, Costa Rica. Folia Primatol (Basel) 84, 102-114.
20
Petrášová, J., Modrý, D., Huffman, M.A., Mapua, M.I., Bobáková, L., Mazoch, V., Singh, J., Kaur, T., Petrželková, K.J., 2010. Gastrointestinal Parasites of Indigenous and Introduced Primate Species of Rubondo Island National Park, Tanzania. Int J Primatol 31, 920-936.
21
Petrasova, J., Uzlikova, M., Kostka, M., Petrzelkova, K.J., Huffman, M.A., Modry, D., 2011. Diversity and host specificity of Blastocystis in syntopic primates on Rubondo Island, Tanzania. Int J Parasitol 41, 1113-1120.
22
Sato, M., Yoonuan, T., Sanguankiat, S., Nuamtanong, S., Pongvongsa, T., Phimmayoi, I., Phanhanan, V., Boupha, B., Moji, K., Waikagul, J., 2011. Short report: Human Trichostrongylus colubriformis infection in a rural village in Laos. Am J Trop Med Hyg 84, 52-54.
23
Singh, K., 2009. Laboratory-acquired infections. Clin Infect Dis 49, 142-147.
24
Sohail, M.R., Fischer, P.R., 2005. Blastocystis hominis and travelers. Travel Med Infect Dis 3, 33-38.
25
Thompson, R.C., Smith, A., 2011. Zoonotic enteric protozoa. Vet Parasitol 182, 70-78.
26