%0 Journal Article %T Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle. %J Archives of Razi Institute %I Razi Vaccine & Serum Research Institute %Z 0365-3439 %A Sotoodehnia, A. %A Moazeni, G. %A Ataei, S. %A Omidi, B. %D 2005 %\ 01/01/2005 %V 59 %N 2 %P 95-101 %! Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle. %K haemorrhagic septicaemia %K oil adjuvant vaccine %K Protection %R 10.22092/ari.2005.103817 %X An oil adjuvant vaccine (OAV) was prepared from a local strain of Pasteurella multocida. Strain 6:B was cultured and inactivated by formalin. Bacterial pellet was prepared by centrifugation and subsequently adjuvanted by Montannide oil ISA-70. A dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by IM route. Animals were bled before and at 24, 90, 150, and 200 days post-vaccination. Collected sera were used in passive mouse protection test (PMPT). Active mouse protection test (AMPT) was carried out for OAV according to standard method. Results of PMPT showed 100% protection up to 150 days and 66-83% up to 200 days post-vaccination. In AMPT, 4 log of protection was gained. In this experiment the immunity induced by OAV adjuvanted by ISA-70 could protect the calves. %U https://archrazi.areeo.ac.ir/article_103817_827e3f7b07c6ccb2c22557b8c4ef2f84.pdf