%0 Journal Article %T Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody. %J Archives of Razi Institute %I Razi Vaccine & Serum Research Institute %Z 0365-3439 %A Ghadakchi, H. %A Dadras, H. %A Pourbakhsh, S.A. %A Hosseini, S.M.H. %D 2005 %\ 01/01/2005 %V 59 %N 2 %P 75-83 %! Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody. %K Infectious bronchitis %K ELISA %K Antibody %K Massachusetts serotype %R 10.22092/ari.2005.103815 %X An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P %U https://archrazi.areeo.ac.ir/article_103815_cd3d7bf7a6fbb979ba92af564ceead93.pdf