%0 Journal Article %T Optimal Timing of Specimen Collection after Rash Onset for Diagnosis of Measles IgM Antibody %J Archives of Razi Institute %I Razi Vaccine & Serum Research Institute %Z 0365-3439 %A Mokhtari Azad, T. %A Naseri, M. %A Yavari, P. %A Gooya, M.M. %A Esteghamati, A. %A Hamkar, R. %D 2005 %\ 02/01/2005 %V 60 %N 1 %P 1-10 %! Optimal Timing of Specimen Collection after Rash Onset for Diagnosis of Measles IgM Antibody %K Measles %K IgM Capture EIA %K Optimal time %R 10.22092/ari.2005.103729 %X   Antibody detection is the most versatile and commonly used method for measles diagnosis. Detection of specific IgM antibodies in a single serum specimen collected within the appropriate time after rash onset can provide a good presumptive diagnosis of current or recent measles infection and is the test of choice for rapid diagnosis of measles cases. So, optimal timing for collection of a single serum specimen to diagnose measles by IgM capture Enzyme Immunoassay (EIA) was evaluated. 399-paired sera were tested for measles IgM antibody. 149-paired sera were measles IgM negative. Two hundred fifty paired sera had at least one IgM positive. 223-paired sera were positive in both first and second samples. 19-paired sample were negative in first and positive in second sample and 8-paired sera were positive in the first and negative in the second samples. 85% and 100% of first specimens within 7 and 7-21 and 94% of all second samples were IgM positive at 28th days after rash onset, respectively. Analysis of data indicates that a single serum specimen collected between 7 to 27 days after rash onset can be used to diagnose most cases of measles with an IgM capture EIA.   %U https://archrazi.areeo.ac.ir/article_103729_c0197333612dc597ab8e4a10e8d89794.pdf