eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
1
9
10.22092/ari.2008.103737
103737
Cross-immunity study of two experimentally oil-emulsion inactivated Infectious bronchitis vaccines in SPF chickens
. Shoshtari
1
M. Vasfi-Marandi
2
R. Toroghi
3
M.H. Bozorgmehrifard
4
R. Momayez
5
Department of Avian Diseases Research and Diagnostic, Razi Vaccine and Serum Research Institute, Karaj, Iran
Poultry Diseases Division, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Research & Biotechnology, Razi Vaccine & Serum Research Institute, Mashhad, Iran.
Poultry Diseases Division, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Avian Diseases Research and Diagnostic, Razi Vaccine and Serum Research Institute, Karaj, Iran
A study has been carried out for determining of cross-immunity between Massachusetts (Mass) M41 and Iranian IR/773/2001 (793/B) strains of infectious bronchitis virus. Two groups of 20 three weeks old SPF chickens vaccinated subcutaneously with two oil-emulsions inactivated Infectious Bronchitis Vaccines (Mass M41 and Iranian IR/773/2001 strains) that had been manufactured in Razi institute in the laboratory scale. A further group of 20 SPF chickens were kept as non-vaccinated control. Four weeks post vaccination half part of the birds of the each vaccinated groups were challenged by eye drop with 103EID50 of M-41 strain of Mass serotype and another part of the birds of the each vaccinated groups were challenged with 103EID50 of Iranian IR/773/2001 793/B strain. The birds of the control group were also divided in two parts and challenged with the viruses (M-41 and IR/773/2001) like the birds of the vaccinated groups. Each of the birds of the vaccinated groups manifested a very strong resistance to homologous virus strains and only 10% of them showed ciliostasis in tracheal section examination. Whereas after challenge with heterologous virus strains, the vaccinated birds with IR/773/2001 killed vaccine revealed 50% resistance against M41 and the birds vaccinated with M41 killed vaccine only showed 30% immunity against IR/773/2001 based on the degree of 50% and 70% ciliostasis in tracheal section examination respectively. All of the control birds showed complete ciliostasis without any protection against the challenge of the both viruses. Upon the results, it is suggested that the distribution of 793/B serotype should be carefully studied in different parts of Iran for appropriate vaccination programmes against infectious bronchitis.
https://archrazi.areeo.ac.ir/article_103737_ecf1e98fb61ba785b67a5ec133f3c9b0.pdf
Infectious bronchitis virus
Cross
Immunity
Massachusetts M41
IR/773/2001 (793/B)
Inactivated oil emulsion vaccine
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
11
17
10.22092/ari.2008.103738
103738
Cloning rhoptry protein 1 (ROP1) gene of Toxoplasma gondii (RH) in expression vector
F. Ghaffarifar
1
A. Dalimi
dalimi4@yahoo.com
2
Z. Eslamirad
3
Z. Sharifi
4
Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning vector for gene analysis and further production of rhoptry proteins. Tachyzoites of the RH strain of T. gondii were harvested from the peritoneal fluid of mice that has been experimentally infected with the parasites. Genomic DNA was extracted by phenol- chloroform method. The ROP1 fragment amplified with specific primers. The purified PCR products were ligated between the EcoR1 and BamH1 sites of the pTZ57R/T cloning vector and transformed into Escherichia coli TG1 strain and screened by IPTG and X-Gal. The plasmid was purified and visualized under UV transilluminator. The amplified fragment was cloned in pTZ57R vector successfully. The correct orientation of the ROP1 fragment was identified by restriction enzyme analysis and sequencing of constructed plasmid. A fragment about 760bp was separated from PTZ57R following digestion and demonstrated on agarose gel electrophoresis.The sequence of this amplified gene showed homology up to 96% with target gene in GenBank database (Accession no. M71274). Recombinant plasmid of ROP1 gene was constructed. It is ready for future study.
https://archrazi.areeo.ac.ir/article_103738_0f5e0ab305fe5cd43a9e3144fa8522fd.pdf
Toxoplasma gondii
Cloning
Rhoptry Protein1 (ROP1) Gene
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
19
28
10.22092/ari.2008.103739
103739
Preparation and in-vitro evaluation of sodium alginate microspheres containing diphtheria toxoid as new vaccine delivery
N. Mohammadpour Dounighi
1
S.A. Mortazavi
2
A. Rezaei Mokarram
3
H. Zolfagharian
4
M.J. Alonso
5
During last two decades, polysaccharides such as alginate (Alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. The aim of the present work was to investigate the potential utility of microparticles made of alginate (Alg) as new vehicles for improving nasal vaccine delivery. For this purpose, diphtheria toxoid (DT) was chosen as a model antigen. DT w as entrapped within microparticles made of Alg of different molecular weight cross-linked using 1M CaCl2 or 3.75 %w/w CaCl2 in n-octanol. DT-loaded microparticles were characterized for their size, loading efficiency and in vitro release of toxoid. The resulting microparticles had a size, which varied depending on formulation conditions and Alg Mw. The results of the in vitro release studies displayed a biphasic release of toxoid, the intensity of the first phase being less pronounced for microparticles cross linked with aqueous CaCl2 than octanolic CaCl2.
https://archrazi.areeo.ac.ir/article_103739_589ce6f3d3fdbbd3447c3ca6c629c657.pdf
Microspheres
Alginate molecular weight
CaCl2 cross linking agent
Diphtheria Toxoid
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
29
34
10.22092/ari.2008.103740
103740
Experimental vaccination of sheep against hydatid cyst using EG95 recombinant vaccine
Gh.R. Motamedi
1
Gh.R. Karimi
2
A.H. Dalimi
3
D.D. Heath
4
H. Paykari
5
Vaccination of livestock with effective vaccine could be one of the control methods for hydatid cyst. In this study a new recombinant vaccine (Eg95) from New Zealand was prepared and used. Thirty health sheep from the same race and identical age and sex were selected. There was no history of any past vaccination or disease in selected animals. Animals were randomly categorized to four groups. One group including ten sheep received two times vaccination and each time 50 microgram with two weeks interval. Another group (10 sheep) received physiological saline without vaccine. Five weeks after second vaccination both groups were challenged with 3500 freshly Echinococcus granulosus (Iran isolate, dog/sheep cycle) eggs intraruminally. Animals, in third and fourth groups, received vaccine and physiological saline respectively. Third and fourth groups did not challenge and kept for vaccine adverse effects or natural infection control. Anti-Eg95 antibody titer was evaluated with ELISA before and two weeks after each vaccination. Optical density (OD) rates for all groups were less than 0.1 before vaccination. A 40 fold increase in OD rates of vaccinated groups was seen 2 weeks after second vaccination. Animals under the study were fully surveyed. Results indicated 98% protection in vaccinated group when they were necropsied 12 months after challenge .The EG95 vaccine can be produced on an industrial scale and can be further use for clinical trial in Iran.
https://archrazi.areeo.ac.ir/article_103740_03e70d4f87dd848a79a6c80efd6b06fc.pdf
Recombinant vaccine
Echinococcus granulosus eggs
Hydatid cyst
Sheep
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
35
40
10.22092/ari.2008.103741
103741
Detection of Ornithobilharzia turkestanikum cercaria (trematoda) by nested-PCR in intermediate host snail, Lymnaea gedrosiana
N. Motamedi
1
R. Salehi Tabar
2
A.H. Dalimi
3
H. Paykari
4
S.A. Ghorashi
5
Gh.R Motamedi
6
Gh.R. Karimi
7
Trematodes are important in economic and public health. Ornithobilharzia turkestanicum (O. turkestanicum) is one of the important economic trematodes in domestic animals. Ornithobilharzia infection in intermediate host (Lymnaea gedrosiana ) can be detected by either exposing snails to light to induce cercarial shedding or by squeezing them between glass slides to detect parasites. The current available diagnostic methods are inefficient for identification of prepatent infections and/or after dead of snails. For the above difficulties we adapted a nested polymerase chain reaction (Nested-PCR) assay for sensitive detection of O. turkestanicum in clinical samples and its cercaria in snails. The life cycle of parasite was maintained in sheep and snails in laboratory in Razi Institute. Adult worms were isolated from sheep and DNA was extracted from worms by a procedure using DNA extraction solution developed in NIGEB. PCR and nested-PCR primers were designed based on 28s ribosomal RNA gene of O. turkestanicum and the DNA was amplified by PCR assay. PCR product was purified and cloned in pTZ57R/T and sequenced. The comparison of the obtained sequences with the GenBank using blast program was sh owed in NCBI Sequence viewer just 682 bp. PCR amplicon was submitted in GenBank and can be assessed using AY862391 accession number. DNA was extracted from the infected and non-infected snails 2-5 days post-infection. The infected snails could be rapidly detected with Nested-PCR. Results indicate that this assay is specific for detecting O. turkestanicum. The high sensitivity of the test enabled identification of single infected snail even when its DNA was pooled with uninfected snails. Thus demonstrating the possibility of mass diagnosis in pools of snails, therefore, the assay has the potential for large-scale demonstration of prepatent infection prevalence in snails and offers a new diagnostic tool for evaluation of bilharziosis trasnsmission and for control of infection as discussed.
https://archrazi.areeo.ac.ir/article_103741_e636fc9bb02783bf405c276716b53791.pdf
PCR
Nested
Diagnosis
Trematoda
Ornithobilharzia turkestanicum
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
41
46
10.22092/ari.2008.103742
103742
Prevalence of Canine Parvovirus (CPV) in diarrheic dogs referred to veterinary hospital in Ahvaz
R. Avizeh
1
B. M. Ghorbanpoor Najafabad
2
B. Mosallanejad
3
R. A. Ronagh
4
This study was performed to determine the prevalence of Canine parvovirus (CPV) in dogs referred to Veterinary Hospital of Ahvaz, Khouzestan province, Iran. Fecal samples were collected from 78 diarrheic dogs between 2005 and 2007. The dogs were divided into two age groups (< 6 months and > 6 months), four different breeds (Terriers, Germanshepherds, Doberman pinschers and Mixed) and another two groups on the basis of clinical signs (hemorrhagic and non-hemorrhagic diarrhea) using Fischer's exact test. Prevalence to CPV (2a or 2b) antigens in these dogs was 16.7% (13 of 78) by means of immunochromatography assay (IC) indicating that this virus is present in ecosystem. The infection had more prevalence in dogs less than 6 months (21.95% 9 of 41) and in breeds of Terriers (26.31% 5 of 19) and German Shepherds (21% 4 of 19), but there were no significant differences between different sexes, age groups and breeds (P>0.05). Nevertheless, infection was significantly higher in hemorrhagic diarrheic dogs (41.38% 12 of 29) (p
https://archrazi.areeo.ac.ir/article_103742_ee14b98f11c4273f31acd0b93c3dbc73.pdf
Canine parvovirus
Immunochromatography
Dog
diarrhea
Ahvaz
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
47
52
10.22092/ari.2008.103743
103743
Preparation of antigens from midgut of Hyalomma anatolicum anatolicum and determining their immunoprotective effects
M. Abdigoudarzi
1
A. Dadmehr
2
F. Golchinfar
f.golchinfar@rvsri.ir
3
R. Madani
4
Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- GM and SDS-TX-GM antigens. The first inoculation on day 0 was administered after emulsification with Freund`s complete adjuvant and second inoculation was given on day 14 with incomplete Freund`s adjuvant and third inoculation was given on day 28 with incomplete Freund`s adjuvant. Blood drawn was from the marginal ear vein on day 38. The humoral immune response was studied by Enzyme Linked Immunosorbent Assay (ELISA) and antibody titers in the sera of immunized rabbits on 10 days after the last booster dose of antigen was 1/32000. After electrophoresis of purified midgut antigens (GS, GM, TX-GM, SDS-TX-GM) 4µg of each were applied on a 10% acryl amide gel and blotted to nitrocellulose paper for reaction with its concern immune sera. By analysis of this Western blot, we got bands of 110, 95, 85, 66, 59, 49, 42 Kda for GSAg, 95, 85, 66, 49, 42 Kda for GM Ag, 66, 49 Kda for TX-GMAg and 66Kda for SDS-TX-GMAg responsible for induction of resistance in the host. The prepared antigens showed engorged nymphs ranged from 32-41%, 29-56%, 29-49% and 29-47% in response to GS, GM, TX-GM and SDS-TX-GM antigens respectively. Immunodominant antigenic proteins of 66 Kda were detected in the sera of rabbits immunized by all above antigens which can be candidate for single immunogen if in future studies show acceptable results. The immunized rabbits were challenged, 10 days after the last booster dose of antigens, with 700 larvae of H. a. anatolicum ticks by releasing them on one ear of rabbits and maximum protection in rabbits against H. a. anatolicum was induced with the one immunized with GSAg.
https://archrazi.areeo.ac.ir/article_103743_580f7540d1046efb449e26d1bf755c50.pdf
Hyalomma anatolicum anatolicum
Midgut
Western blot
ELISA
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2008-07-01
63
2
53
56
10.22092/ari.2008.103744
103744
Study on chemotherapeutic value of imidorazi against sheep babesia infection
R. Hashemi-Fesharki
1
K. Esmaeilnia
2
The activity and efficacy of Iranian synthesized imidocarb dipropionate (imidorazi) has been tested against Babesia ovis infection in experimentally and naturally affected sheep. The results indicated that the drug is effective and could be used for treatment of sheep and goats babesiosis. The effectiveness of imidorazi is also similar to the imizol (imidocarb dipropionate) imported from abroad.
https://archrazi.areeo.ac.ir/article_103744_cc1228764cd1eba46509ca446a4d33a4.pdf
Babesiosis
Chemotherapy
Imizole
Imidorazi
Feld trial