@article { author = {Alamian, S. and Zahraei Salehi, T. and Aghaiypour Kolyani, K. and Esmaelizad, M. and Etemadi, A.}, title = {Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains}, journal = {Archives of Razi Institute}, volume = {74}, number = {3}, pages = {235-241}, year = {2019}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2018.122128.1218}, abstract = {Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This study aimed to present a novel conventional and real-time polymerase chain reaction (PCR) assay for the identification of Brucella abortus strains. Regarding this, two primers (bru ab2) were designed based on the unique loci encoding autotransporter-associated beta strand repeat-containing protein (ID:YP00113760). A total of 56 Brucella strains (e.g., reference, vaccinal, and field isolates) and Yersinia enterocolitica, as a non-Brucella isolate, were evaluated in conventional and real-time PCR systems. The results of the study indicated that 0.4 ng and 400 FG of genomic DNA of B. abortus strains can be detected by conventional and real-time PCR, respectively. The primers, bru ab2, were suitable for both PCR methods. Both methods were specific for the detection of all strains of the bacterium; however, real-time PCR assay was 1000-fold more sensitive than the conventional PCR method. Therefore, this new detection system could be a suitable selective modified method for the accurate identification of all B. abortus strains.}, keywords = {Brucella abortus,identification,PCR,Real-time PCR}, url = {https://archrazi.areeo.ac.ir/article_119270.html}, eprint = {https://archrazi.areeo.ac.ir/article_119270_f48a27d3fcdadad8929ee276ace930df.pdf} }