@article { author = {Broomand, Z. and Jafari, R. A. and Mayahi, M.}, title = {Detection of Newcastle Disease, H9N2 Avian Influenza, and Infectious Bronchitis Viruses in Respiratory Diseases in Backyard Chickens in Ahvaz, Iran, in 2014-2015}, journal = {Archives of Razi Institute}, volume = {73}, number = {1}, pages = {19-25}, year = {2018}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2018.114056}, abstract = {Newcastle disease virus (NDV), avian influenza virus (AIV), and infectious bronchitis virus (IBV) are the most prevalent viral pathogens in the Iranian poultry industry. This study aimed to reveal the presence of these viruses in the backyard chickens in Ahvaz, located in the Southwest of Iran. A total of 100 chickens with respiratory signs and mortality were examined by taking the blood samples as well as tracheal and cloacal swabs. Most of the chickens had not received any vaccine. The blood samples were assessed for the antibodies against NDV and AIV by haemagglutination inhibition test, and against IBV by enzyme-linked immunosorbent assay. The swab samples were utilized for molecular detection using reverse transcription polymerase chain reaction (RT-PCR). Based on the results of the serologic test, 77%, 45%, and 38.4% of the birds were positive for NDV, AIV, and IBV, respectively. In the RT-PCR, 95% of the birds were positive for one of the three viruses. The detection rates of NDV, AIV, and IBV were 60%, 34%, and 55%, respectively. The coinfections of AIV/NDV, AIV/IBV, NDV/IBV, and AIV/NDV/ IBV were observed in 13%, 4%, 23%, and 7% of the sampled chickens, respectively. The results demonstrated that the Iranian backyard chickens were infected with NDV, AIV, and IBV. This could pose a threat to the commercial poultry; therefore, preventive measures need to be implemented in this regard.}, keywords = {Backyard chickens,Infectious bronchitis,Influenza,Newcastle disease}, url = {https://archrazi.areeo.ac.ir/article_114056.html}, eprint = {https://archrazi.areeo.ac.ir/article_114056_d2b54266c50fb3f45e56282c2d6e571a.pdf} }