@article { author = {Namavari, M.M. and Sayari, M. and Kargar, M. and Lotfi, M. and Kamalzadeh, M.}, title = {Virulence assessment of a Neospora caninum isolate for inbred C57BL/6 mouse}, journal = {Archives of Razi Institute}, volume = {67}, number = {1}, pages = {57-61}, year = {2012}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2016.103888}, abstract = {Neospora caninum (N. caninum) is an apicomplexan parasite and causes abortion and congenital neosporosis in cattle worldwide. In this study, we evaluate the virulence of a N. caninum isolate on mouse strain C57BL/6. Six groups of five mice C57BL/6 were intraperitoneally inoculated with 1 × l07, 1.5 × l07, 2 × l07, 3 × l07 and 4 × l07 tachyzoites and a control inoculum of DMEM, respectively. Clinical signs and mortality rate were recorded and confirmed by histopathological findings and molecular method. The results of this study indicated that LD50 was 2.5 × l07 tachyzoites of N. caninum per C57BL/6 mouse. This can be used as a lethal challenge model in vaccine development studies.}, keywords = {Neospora caninum,C57BL/6 mouse model,LD50}, url = {https://archrazi.areeo.ac.ir/article_103888.html}, eprint = {https://archrazi.areeo.ac.ir/article_103888_8b19d5e3fb572078b39692c67181fc48.pdf} }