@article { author = {Ranjbar, M. M. and Ataei, S. and Nikbakht Brujeni, Gh. and Golabdar, S.}, title = {Analysis of variations, structures, and phylogenic characteristics of bovine leukocyte antigen DRB3 exon2}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {147-157}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111611}, abstract = {Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular andevolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustrate some novelpractical evidence on interspecies diversity, the resistance /susceptibility points in cattle breeding, and vaccinedesign. Initially, BoLA-DRB3.2* alleles and orthologous exons in the selected livestock were retrieved andchecked. In the next step, the secondary/tertiary structure of BoLA-DRB3.2*24 gene product was modeled andvalidated. Then, hypervariable regions (HVRs) of alleles were identified by hybrid approaches. In the last step,interspecies relationship, allele’s phylogeny/grouping, and estimate of average evolutionary divergence wereexplored. Shannon entropy variation analysis showed eight HVRs and three semi-variable regions in BoLADRB3.2*alleles. These HVRs were present in all the three sub-structures and dominantly existed in alpha helix.In addition, strong relationships and little diversity were noted in phylogenetic trees of cattle, buffaloes, sheep,and goats. Furthermore, there was some evidence on divergence of DRB3 before speciation among thementioned species and possibility of cross prediction resistance/susceptibility alleles. Finally, DRB3 alleles weregrouped into seven clusters, and older and newer alleles were identified. The results show that similar studiesshould be done in other animals to better understand the nature of the DRB3 attributes.}, keywords = {BoLA-DRB3.2,Cattle,Variation,modeling,phylogeny}, url = {https://archrazi.areeo.ac.ir/article_111611.html}, eprint = {https://archrazi.areeo.ac.ir/article_111611_50130e38448fdbd64a0f96493963014f.pdf} } @article { author = {Pourbakhsh, S. A. and Abtin, A. R. and Ashtari, A. and Kheirkhah, B. and Bayatzadeh, M. A. and Ahangran, Salimeh}, title = {Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {159-164}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111610}, abstract = {Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease ingoats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semensamples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented forM. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was appliedto detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bpfragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed forboth the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positiveand typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. Inaddition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the sampleswere shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M.agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended toconcern semen as one of the significant sources for this pathogen and the possibility for transmission to thefemale goats through semen is highlighted. Moreover, presence of this microorganism in semen could beinvolved in infertility of goat population.}, keywords = {Goat buck,Lipoprotein gene,Mycoplasma agalactiae,Semen,16srRNA}, url = {https://archrazi.areeo.ac.ir/article_111610.html}, eprint = {https://archrazi.areeo.ac.ir/article_111610_714d06eb229f24c70e07977dcce074e6.pdf} } @article { author = {Mirhaghgoye Jalali, S. F. and Jabbari, A.R. and Esmael Zad, M.}, title = {LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis loci}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {165-171}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111607}, abstract = {Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic typing systems.The aim of this study was to identify the LPS genotypes of the ovine P. multocida isolates obtained from pneumonia cases in Iran. The LPS genotype of the isolates was determined using eight specific primers for LPS outer core biosynthesis loci. The LPS genes were amplified by polymerase chain reaction (PCR), then they were sequenced and compared to the sequences registered in the GenBank. Of the 32 ovine P. multocida isolates tested, 21 (65.62%) isolates belonged to genotype L6, 9 (28.12%) isolates contained genotype L3, 1 (3.12%) isolate had both L3 and L6 loci, and 1 (3.12%) isolate remained untypeable. The LPS-PCR was able to type 31 of 32 field ovine isolates from Iran. According to the phylogenetic analysis, L3 genotype isolates were grouped into two distinct lineages. LPS gene sequences among L6 genotypes of ovine P. multocida isolates from Iran and the related sequences in the GenBank were highly similar (>99.5%). LPS-PCR is an accurate genotyping method that was able to classify P. multocida strains into one of the eight distinct LPS genotypes.}, keywords = {P. multocida,Sheep,LPS outer core,PCR-typing}, url = {https://archrazi.areeo.ac.ir/article_111607.html}, eprint = {https://archrazi.areeo.ac.ir/article_111607_ec494786bf8c99c264e682d2b81fb9f7.pdf} } @article { author = {Zandieh, S. and Lotfi, Mohsen and Kamalzadeh, M. and Shiri, N. and Parmour, E. and Eshaghi, A. and Masoudi, S. and Hablolvarid, M. H. and Shoushtari, A. and Goudarzi, H. and Taher Mofrad, S. M. J. and Amanpour, S.}, title = {The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {173-179}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111601}, abstract = {Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.}, keywords = {Infectious bursal disease,Gumboro Disease,Chicken embryo fibroblast,Ovine lymphoid origin cell line,Cell characterization}, url = {https://archrazi.areeo.ac.ir/article_111601.html}, eprint = {https://archrazi.areeo.ac.ir/article_111601_97eff51c98a27271fec42421e8fd8759.pdf} } @article { author = {Razmaraii, N. and Babaei, H. and Mohajjel Nayebi, A. and Ahdi Khosroshahi, S. and Farajnia, S.}, title = {Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {181-187}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111609}, abstract = {Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.}, keywords = {Antioxidant activity,Crocin,Cytotoxicity,Grape Seed Extract,Phenytoin}, url = {https://archrazi.areeo.ac.ir/article_111609.html}, eprint = {https://archrazi.areeo.ac.ir/article_111609_eaa7514b51ade19804e6ce5adb392c25.pdf} } @article { author = {Narimani, B. and Hoghooghi-Rad, N. and Shayan, P. and Rahbari, S.}, title = {Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {189-195}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111605}, abstract = {Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect T.annulata DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for Theileria spp. and T.orientalis amplification, respectively. The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both T.annulata and T.orientalis. Out of 291 buffaloes, four samples (1.4%) were infected by Theileria genotypes, and two buffaloes (0.7%) were infected only by T.orientalis. The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that T.orientalis may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with T.orientalis for the first time.}, keywords = {Buffalo,Cattle,Iran,PCR,Theileria orientalis}, url = {https://archrazi.areeo.ac.ir/article_111605.html}, eprint = {https://archrazi.areeo.ac.ir/article_111605_c7d2f7bd39cc7dad9904aa5ed56e8e0a.pdf} } @article { author = {Dalimi, A. and Jameie, F. and Mohammadiha, A. and Barati, M. and Molaei, S.}, title = {Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {197-201}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22034/ari.2017.108389}, abstract = {Hepatozoon species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect Hepatozoon canis in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of 104 blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, 18S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of 897 bp for all the positive samples. Twenty-four out of the 104 (23.07%) samples were found to be positive for H. canis. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of 99% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of H. canis from Iran.}, keywords = {Hepatozoon canis,PCR,Dog,Ardabil,Iran}, url = {https://archrazi.areeo.ac.ir/article_108389.html}, eprint = {https://archrazi.areeo.ac.ir/article_108389_5b5fffbfaebb8b3e8171aa9a5440e53d.pdf} } @article { author = {Soltani, Z. and Keshavarzi, D. and Ebrahimi, M. and Soltani, A. and Moemenbellah-Fard, M. J. and Soltani, F. and Faramarzi, H. and Amraee, K. and Elyasigomari, A.}, title = {The Fauna and Active Season of Mosquitoes in West of Fars Province, Southwest of Iran}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {203-208}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111603}, abstract = {Culicidae are highly important for public health as they can be vectors of diseases and are responsible for a wide spectrum of infections. Five collection sites were selected randomly with regards to existing facilities in Firouzabad County. For collecting larvae and total catch for adult mosquitoes, sampling was carried out by dipping technique for collecting larvae and total catch for adult mosquitoes. A total of 689 adults and 1313 larvae of Culicidae were collected, of which 3 genera and 6 species of Culicidae were recognized, namely, Anopheles superpictus, Anopheles d’thali, Culex sinaiticus, Culex theileri, Culex mimeticus, and Culiseta longiareolata. Cx. theileri was the most frequent Culicidae collected at Firouzabad, with a total of 613 and 247 larval and adult specimens, respectively. The highest number of mosquitoes was collected in June (31.1%) and the lowest in May (3.4%). The mean temperatures in June and May were 31.3˚C and 28.2˚C, respectively. We found some vectors that are of medical and veterinary importance; our results could be applied in vector control programs that aim at eradication or control of mosquitoes in this area.}, keywords = {Culicidae,Monthly frequency,Fars,Southwestern Iran}, url = {https://archrazi.areeo.ac.ir/article_111603.html}, eprint = {https://archrazi.areeo.ac.ir/article_111603_9f639dd4d19caf45e13b7d75b026e69f.pdf} }