@article { author = {Asli, E. and Taghizadeh, M. and Rezaei Mokaram, A. and Ebrahimi, S.M. and Zavaran Hoseini, A. and Tebianian, M.}, title = {Cloning and Expression of Mycobacterium Tuberculosis ESAT-6 in Prokaryotic System}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {1-7}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103800}, abstract = {  The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis. Selected gene (Rv3875) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQE30-ES plasmid was constructed. This hybrid vector was transformed in E. coli M15 and expressed in optimal condition. The expressed protein was analyzed on SDS-PAGE and confirmed by western blotting using specific antisera to ESAT-6. We successfully cloned and expressed ESAT-6 (His)6 from M. tuberculosis H37Rv genome. As well as usage for serodiagnosis, this recombinant protein offers the potential development of other vaccine formats such as DNA or subunit vaccines against tuberculosis.}, keywords = {}, url = {https://archrazi.areeo.ac.ir/article_103800.html}, eprint = {https://archrazi.areeo.ac.ir/article_103800_15aeeca606c8b468b5347dd5db8fdc36.pdf} } @article { author = {Hashemi-Fesharki, R. and Bozorgi, S. and Esmaeil Nia, K. and Habibi, G.R. and Bordbar, N.}, title = {Semi-quantitative Analysis of Expression of Various Genes in relation to Possible Markers for Theileria annulata Attenuation}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {9-17}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103801}, abstract = {  The sporozoites of Theileria annulata invade bovine MHC II cells, where they differentiate into schizonts. The later can immortalize and induce fundamental changes in their host cells. Live attenuated vaccine is an important way of controlling T. annulata infection of cattle. Production is by prolonged cultivation of macroschizont-infected cells. The mechanisms underlying this transformation are not understood. The objects of this work were to analyze the expression levels of MMPs, Pro-inflammatory cytokines, Tams1 and TashHN genes in relation to possible markers for Theileria annulata attenuation. Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in gene expression level among different passage numbers of three cell lines. The results of this study demonstrated that the infected cells show detectable specific transcripts for MMP9 in low passage cultures, but it decreased in long term passages (S15 vaccine strain and high passage number of C1 and C2 cell lines). The analyses of three available cell lines indicated detectable amount of specific mRNAs for TashHN. Tams1 specific transcripts were detected in low passage number of C1 and C2 cell lines, but not obtained in attenuated S15 vaccine and prolonged culture of C1 and C2 cell lines. Two pro-inflammatory cytokines, IL-1-beta and TNF-alpha, were detected with high fluctuations in all three T. annulata infected cell lines, both in low and high passage number. In conclusion, the results of this work clearly showed that the level of MMP9 transcripts is in contrast with the amounts of Tams1 mRNAs in T.annulata schizont infected cell lines that might be considered for virulence and attenuation respectively. Understanding the mechanisms of virulence and attenuation of infected cell line by using molecular biology methods and in vivo animal experiments could help to increase our knowledge about attenuation mechanisms and preparing and identifying appropriate cell lines in order to develop the new T. annulata vaccine cell lines.}, keywords = {Theileria annulata,Gene expression,Matrix metalloproteinase,cell line,attenuation,vaccine}, url = {https://archrazi.areeo.ac.ir/article_103801.html}, eprint = {https://archrazi.areeo.ac.ir/article_103801_67755f23de67f29c7d2352c18388f869.pdf} } @article { author = {Shooshtari, A.H. and Pourbakhsh, S.A. and Aghaiypour, K. and Keivanfar, H. and Varshovi, H.R. and Aghaebrahimian, M.}, title = {Capripoxvirus identification by PCR based on P32 gene}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {19-25}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103802}, abstract = {  Sheeppox virus (SPV) and goatpox virus (GPV) belong to the capripoxvirus genus of Poxviridae family. Sheeppox and goatpox along with contagious ecthyma (CE) are endemic diseases in Iran. Capripox laboratory conformation based on virological and serological techniques are time consuming, laborious and most of them of low specificity, because of close antigenic relationship between capripoxvirus and parapoxvirus. The aim of this study was to develop a capripoxvirus specific PCR assay for SPV & GPV identification on the basis of 390 bp fragment of P32 gene encoding capripoxvirus immunodominant antigen. PCR reaction was optimized using two reference strains of SPV & GPV and four field isolates in tissue culture supernatants. The identity of PCR product was confirmed by sequence analysis and the sensitivity of PCR was performed with 10 fold serial dilutions genomic LK cell DNA infected with capripoxvirus. This PCR was carried out on Twenty-nine biopsy samples from different organs of sheep and goats suspected to SPV & GPV against six biopsy samples infected with CE . Twenty-five SPV & GPV samples were positive and all of CE samples were negative. This PCR assay showed high sensitivity in detection of capripoxvirus DNA and good specificity in differentiation of capripoxvirus from parapoxvirus.}, keywords = {Capripoxvirus,PCR,P32 gene}, url = {https://archrazi.areeo.ac.ir/article_103802.html}, eprint = {https://archrazi.areeo.ac.ir/article_103802_79f73a0bd09c6741947152d544867f0f.pdf} } @article { author = {Mahmodi, M. and Mohaghegh Hazrati, S. and Latifynia, A. and Mohebali, M.}, title = {Study on immunity of Leishmania major new crude antigen as a vaccine against leishmaniasis in out bred (resistant) and Balb/c (sensitive) mice}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {27-37}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103803}, abstract = {The aim of this study was to prepare a new formula of Leishmania major (L. major) crude antigen and evaluate its effect on immune system. For this purpose L. major promastigotes were cultured, harvested, washed, and resuspended in physiologic saline and the suspensions were dispersed in five equal batches. 0.1 ml of various doses of the cocktail antigen were injected intradermaly in three groups of mice [Ninety out bred resistant mice (designated type 1 mice) and 90 Balb/c sensitive mice (designated type 2 mice) of both sexes with age of three months] group I received antigen and a booster dose of the same antigen, group II received Leishmania antigen containing Bacillus Calmette and Guerrin (BCG), group III inoculated with antigen containing BCG and a booster dose of the same antigen, group IV remained intact, groups V and VI received solely BCG, and BCG solvent, respectively without any antigen. Delayed Type Hypersensitivity (DTH) response and spleen white pulp follicles (SWPF) status were evaluated. No significant differences among groups IV, V and VI were seen in two types of mice regarding PPD skin test, in leishmanin skin tests and spleen white pulp statue. However there was a significant difference among three groups of two mice types received the antigens in a dose dependent manner (P}, keywords = {Leishmania major,Antigen,vaccine}, url = {https://archrazi.areeo.ac.ir/article_103803.html}, eprint = {https://archrazi.areeo.ac.ir/article_103803_aea8a74249d811ace07ff24a3a9ef01e.pdf} } @article { author = {Pirstani, M. and Sadraei, J. and Dalimi, A. and Vaeznia, H.}, title = {Determination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {39-44}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103804}, abstract = {Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) of the mini-exon gene. Our results showed that the Leishmania tropica was more prevalent in Mashhad where among 50 isolates, 17 were detected Leishmania major (34%) and 38 samples were Leishmania tropica (66%).}, keywords = {Leishmania major,Leishmania tropica,PCR,RFLP,mini,exon gene}, url = {https://archrazi.areeo.ac.ir/article_103804.html}, eprint = {https://archrazi.areeo.ac.ir/article_103804_ab318dfacc68fe185f53c5e8c7215d18.pdf} } @article { author = {Razmi, G.R. and Hashemi Tabar, G.R. and Maleki, M.}, title = {In vivo effect of albendazole and mebendazole on hydatid cyst of mice}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {45-50}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103805}, abstract = {In current study, a few sheep cystic livers and lungs were obtained from Mashhad slaughter house and protoscolex were separated aseptically. Thirty 6-week-old Swiss mice were divided into 5 groups and two thousands of live protoscolices were inoculated intrapritoaneally in each mouse. In group 1 and 2 (prophylactic groups), mice were given 150 mg/kg of oral albendazole and mebendazole for 10 days. In group 3 and 4 (treated groups), mice were treated orally with 300 mg/kg of albendazole and mebendazole for 22 days with an interval of 2 days after every 4 days of treatment 6 months after inoculation. The control group (group 5), was sham injected with normal saline. Mice were killed after 7 months and internal organs were observed for hydatid cyst. In group 1, 2 cysts were observed in the liver of mice. In this group, although albendazole did not prevent cyst formation, but the number and also the size of the cysts were lower and smaller than the control group. In group 2, 3 cysts were observed in internal organs. In group 3, there was no cyst in internal organs and so in this group it is concluded that albendazole prevented cyst formation. In group 4, 1 cyst was observed on the liver of mice. In control group a lot of cysts were observed in internal organs of mice and the average size of cysts were bigger than prophylactic and also treatment groups.}, keywords = {Hydatid cyst,Albendazole,mebendazole,Mouse}, url = {https://archrazi.areeo.ac.ir/article_103805.html}, eprint = {https://archrazi.areeo.ac.ir/article_103805_8c7868c0d279cd8501fa6b45b3dafe3d.pdf} } @article { author = {Hashemlou, M. and Zare Mirakabadi, A. and Ahmadi, M. and Hejazi, M.}, title = {Study on anti inflammatory effect of scorpion (Mesobuthus eupeus) venom in adjuvant-induced arthritis in rats}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {51-56}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103806}, abstract = {Rheumatoid arthritis is an autoimmune disease that causes chronic inflammation of the joints as well as other organs in the body. Adjuvant-induced arthritis models in inbred rats serve as relevant models for RA, having many clinical similarities to this disease. Using honey bee venom as a treatment for Rheumatoid arthritis is an ancient therapy in various parts of the world. However scorpion venom neurotoxins are responsible for toxicity and pharmacological effects. Twenty-five Wistar male rats weighing 110-130 g were divided in 5 groups and Arthritis was induced in them, using Freund’s adjuvant, except in group 1. In group 2 after the induction of arthritis no treatment was given. Group 3 received Betamethasone as an anti-inflammatory medicine. Venom (5µg/rat) was used in group 4 as a treatment and Group 5 received crude venom (10µg/rat) as treatment, after R.A induction, all the animals received treatment near the site of tibio-tarsal joint subcutaneously. The clinical features of the adjuvant induced arthritis like difficulty in movement and edema in joint appeared 3 days after inoculation of adjuvant. The onset of inflammation was explosive occurring 13-15 days post inoculation with a peak onset at day15. After the treatment of rats, there was a significant reduction in score of arthritis index in all treated animals. The changes in size of tibio-tarsal joint region in groups 4 and 5 which received crude scorpion venom and group 3 with Betamethasone treatment after arthritis development decreased. At the end of experiment, blood collection for WBCs count was carried out. In 2 (untreated rats) and Betamethasone treated rats, there was a significant rise in WBCs count. However in venom treated rats the rise in WBCs was not significant as compared to group 1 rats. The present study demonstrated that the scorpion (Mesobuthus eupeus) venom could be effective as anti-arthritis agent in animal model of acute inflammation. More studies are needed to be carried out to find the mechanism of the venom and exact therapeutic doses of the venom for acting as anti-arthritis agent.}, keywords = {: Mesobuthus eupeus,Scorpion venom,Anti,arthritic effect,inflammation,Adjuvant,induced arthritis}, url = {https://archrazi.areeo.ac.ir/article_103806.html}, eprint = {https://archrazi.areeo.ac.ir/article_103806_77f318a1d16b0e756c880520a189d574.pdf} } @article { author = {Moazeni Jula, G. and Hablolvarid, M.H. and Jabbari, A.R.}, title = {Experimintal Study of Peracute Fowl Cholera due to Pasteurella multocida Vaccinal Strain (serotype A1) in Chickens}, journal = {Archives of Razi Institute}, volume = {64}, number = {1}, pages = {57-60}, year = {2009}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2009.103807}, abstract = {In order to show the type and severity of gross and histopathologic lesions induce by vaccinal strain (serotype A1) of Pateurella mulocida, ten four-week-old SPF chickens were inoculated intramuscularly with 75 cfu of ( 0.5 ml of 10-7 dilution) bacterium. All birds died in less than 16 hours. No prominent gross lesions were observed in different organs. In microscopic examination, the most common histopathological findings were as congestion and hemorrhage. Moreover, large amount of viscid mucus were observed in digestive tracts.}, keywords = {fowl cholera,Histopathology,Pasteurella multocida,Chicken}, url = {https://archrazi.areeo.ac.ir/article_103807.html}, eprint = {https://archrazi.areeo.ac.ir/article_103807_92ad212ccf1d60e5dd8f8e8535af8703.pdf} }