Unveiling indirect ELISA test against nucleoprotein of H9N2 comparing with hemagglutination inhibition test

Document Type : Original Articles

Authors

1 Department of Proteomics and Biochemistry, Razi vaccine and serum research institute, Agricultural research education and extension organization (AREEO), Karaj, Iran

2 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

10.22092/ari.2024.364656.3002

Abstract

Influenza is an acute and highly contagious respiratory disease caused by an RNA virus belonging to the Orthomyxoviridae family. This virus can infect birds and mammals. Avian influenza is an infection or a syndrome caused by type A influenza viruse. Some strains, such as Asian H9N2 cause respiratory diseases in people in Asia. Therefore, the purpose of this study is to diagnose this disease quickly and easily.
Developing an indirect ELISA test by using nucleoprotein of the H9N2 A/Chicken/Iran/259/2014 virus with a molecular weight of 60 kilo Daltons, which was separated from the virus by the electroelution method with use of the monoclonal antibody against nucleoprotein as a standard. After that, the results of the indirect ELISA test and the hemagglutination inhibition tests were compared using 300 serum samples of birds.
The results of this research demonstrates the relationship between indirect ELISA test and the hemagglutination inhibition when compared. A Spearman's Correlation indicated that there was a significant and strong positive relationship (ρ =0.901, p < .001, N = 300). Our indirect ELISA test showed a sensitivity of 90% and a specificity of 92%.
Since the disease with mild symptoms can make the diagnosis difficult, we need to control and quickly identify the avian influenza virus. Our indirect Elisa test could help detect a wide range of strains by utilizing a conserved antigen as well as being able to be used for screening more serum-suspected samples in a time efficient manner as compared to the golden standard test, hemagglutination inhibition.

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Archives of Razi Institute

Articles in Press, Accepted Manuscript
Available Online from 13 April 2024

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