Antioxidative potential and activity of potassium polyacrylate and coenzyme Q10 on rat hepatic mitochondrial permeability transition pores

Document Type : Original Articles

Authors

1 Department of Biochemistry, Faculty of Science, Federal University Oye-Ekiti

2 Department of Biochemistry, University of Ibadan

3 1INICSA, Enrique Barros Pabellón Biología Celular, Ciudad Universitaria, X5000, Córdoba, Argentina

4 Department of Biochemistry, Federal University Oye-Ekiti, Nigeria

10.22092/ari.2023.363782.2893

Abstract

Multiple biological activities of coenzyme Q10 have been demonstrated, opening up opportunities for research and development. The biological action of potassium polyacrylate and its impact on the mitochondrial permeability transition (mPT) pores, however, are both poorly understood. Therefore, this study investigated the in vitro antioxidative potential of potassium polyacrylate (PCK) and coenzyme Q10 (CoQ10) and their effects on mitochondrial permeability transition pores. In vitro antioxidant and angiotensin-converting enzyme inhibitory activities were determined using standard procedures, and lipid peroxidation was also determined. Mitochondrial swelling was assessed as the change in absorbance under succinate-energized conditions. Cytochrome c release and mitochondrial ATPase activity were assessed as previously reported. The results showed that PCK and CoQ10 significantly scavenged DPPH and nitric oxide radicals in a concentration-dependent manner and exhibited a greater ferric-reducing antioxidant potential. PCK showed a high DPPH radical scavenging ability with the lowest IC50 value of 54.05 µg/mL while CoQ10 exhibited higher reducing power with the IC50 value of 82.14 µg/mL. It was also observed that they both inhibited angiotensin-converting enzyme activity. Moreover, PCK and CoQ10 significantly (p<0.05) prevented lipid peroxidation, modulated the opening of mitochondrial permeability transition (mPT) pores and caused no significant release of cytochrome c. However, CoQ10 showed a mild inductive effect on mPT pores at higher concentrations. PCK and CoQ10 also enhanced mitochondrial ATPase activity. The findings from this study suggest that both PCK and CoQ10 could be useful in the management of diseases in which excessive apoptosis is characterized by excessive tissue degeneration, such as neurodegenerative conditions.

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Archives of Razi Institute

Articles in Press, Accepted Manuscript
Available Online from 24 February 2024

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