Bradykinin Potentiating Factors of Venom from Iranian Medically Important Scorpions

Document Type: Original Articles

Authors

1 Central Laboratory, Biotechnology Department, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research

2 Assistant professor of Biotechnology Department, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

The venom of animals, including snakes, scorpions and spiders is a complex combination of proteins, peptides, and other biomolecules as well as some minerals. Among the biomolecules of some animal’s venom, small peptides that lack disulfide bands known as Non-Disulfide Bridge Peptides (NDBPs) potentiate the bradykinin by prevent converting of angiotensin 1 to angiotensin 2 with the mechanism of inhibiting the Angiotensin Converting Enzyme (ACE) activity and finally reducing the blood pressure in the victims. This feature of the NDBPs of animal’s venom suggests them as the potential of biological drugs. The aim of the present study was to isolate venom components of the three species of Iranian medically important scorpions and to study the bradykinin potentiating effect of them.
The scorpion specimens were prepared from venomous animals and antivenom production department of Razi Vaccine and Serum Research Institute (RVSRI), and venom extraction were performed by electrical shock (5 volt). The obtained liquid venom of three species specimens were freezed and lyophilized immediately, and then preserved in cool and dried place. Isolation of the venom components for each scorpion was carried out using high performance liquid chromatography (HPLC). The obtained ranges of venom fractions (zones) were tested on isolated tissues of Guinea pig ileum and rat uterus using organ bath instrument in several replicates. The bioassays were resulted in the peptides including Z1 and Z2 regions of venom fractions of the Hottentotta sulcyi, Z2 for Odontobutus doriea and Z2 and Z3 in Mesobutus eupeus venom fractions demonstrated bradykinin potentiating effect. It is concluded that Bradykinin Potentiating Factors (BPFs) were traceable in the venom of all three scorpion species. Therefore, these venoms have the therapeutic potential to exploit biological based drugs.

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