Detection and Isolation of H9N2 subtype avian influenza virus in House sparrows (Passer domesticus) of Ahvaz, Iran

Document Type: Original Articles

Authors

1 shahid chamran university ahvaz

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

3 Professor of department of Clinical science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran.

4 shahid chamran university of Ahvaz

Abstract

Avian influenza (AI) is an acute infectious disease with worldwide significance and causing extensive economic losses in the poultry industry. Avian influenza viruses are members of the family Orthomyxoviridae and placed in the genus influenza virus A. These viruses have been isolated from more than 90 species of free-living birds. Migratory birds are reservoirs for AI viruses and major agents responsible for the global outbreaks. The Passeriformes are found in most parts of the world, and cover a variety of habitats from rural to urban areas. House sparrows are a varied of the family Passeridae. Due to their free flying, they are strongly associated with seabirds, indigenous and industrial poultry. The aim of this study was to determine the role of house sparrows in Avian Influenza virus (H9N2) circulation in the Ahvaz region. The intestinal and tracheal samples were taken from 200 sparrows around Ahvaz during 2017.Reverse transcriptase polymerase chain reaction (RT-PCR) was performed using specific primers in order to detect M and H9 gene of avian influenza viruses. The positive specimens in M gene PCR were inoculated into 9-11-day-old embryo chicken eggs via the allantoic fluid. The results showed that 11 out of 200 samples were positive for two gene (M and H9). According to the results of this study, house sparrows are infected to H9N2 and pose a threat to commercial poultry. Therefore, this is important to be considered in preventive measurements. these birds may play a significant role in the transmission of influenza viruses between wildlife and domestic animals.

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