Evaluation of influence of Zeolite/Collagen nano composite (ZC) and hydroxyapatite (HA) on bone healing: A study on rabbits

Document Type: Original Articles

Authors

1 Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 assistant professor /science and research university of tehran

3 Associate Professor of Veterinary Medicine (Veterinary Surgery), Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

4 هیئت علمی دانشگاه علوم نوین پزشکی.دانشگاه علوم پزشکی تبریز

5 Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Bone healing is still a great challenge in orthopedic surgery in clinical practice. The literature is poor regarding effect of Zeolite/Collagen nanocomposite on bone regeneration. In the present study, a critical segmental defect of rabbit femur was repaired using defects in femurs repaired by Zeolite/Collagen nanocomposite and the effects were examined histologically. Forty-five rabbits (white New Zealand) roughly seven months old and weight of 3.5 kilograms was used. After making the bone defects, all animals were randomized into three groups and each group included 15 animals. In normal control group (NC) a defect was created and no intervention was made and the skin incision was sutured. In the zeolite/collagen group (ZC) the nanocomposite of zeolite/collagen was placed into the created defect. In the hydroxyapatite group (HA) the hydroxyapatite was place into the created defect. The samples were collected on days 15, 30, and 45 postoperatively and assessed histopathologically. Average scores of the index of union were compared and considerable alterations were observed among the values of index of union in experimental groups (p<0.05). The values of index of spongiosa demonstrated that on day 15 it was the highest in zeolite/collagen group. (2.2) and it was the lowest in hydroxyapatite and normal control groups. (0.6). The values of index of Bone marrow demonstrated that there was not a noticeable alteration among the values of index of bone marrow in the experimental groups (P>0.05). The findings of our investigation demonstrated that zeolite/collagen nanocomposite might be considered for reconstruction in bone damages. It seems the zeolite/collagen nanocomposite bears a crucial capability in the reconstruction of bone damages and might be used as a biological frame in bone damages.

Keywords

Main Subjects