Molecular Detection of Gamma Coronaviruses in Birds Parks, Iran

Document Type: Original Articles

Authors

1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

2 69 Azadi Ave

3 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

4 IVO

5 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj, I.R.IRAN.

6 Professor of department of Clinical science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran.

7 مسوول آزمایشگاه اپیدمیولوژی موسسه رازی

8 Department of Pathobiology, School of Veterinary Medicine, Shiraz University

Abstract

Gamma Coronaviruses (GCoVs) are distributed worldwide, highly infectious, and difficult to control because of their extensive genetic diversity, short generation time, and high mutation rates and are found in a wide variety of wild and domestic animals, and are considered as a zoonotic and emerging infectious disease.
There has been no molecular surveillance data to describe the epidemiology of GCOVs in other avian species. The aim of the present study was the detection of GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight different bird species ((Chickens (Gallus gallus), Pheasant (Phasianus colchicus), Turkey (Meleagris gallopavo), Partridge (Perdix perdix), Quail (Coturnix coturnix), Duck (Anas platyrhynchos), Goose (Anserini),and Guinea fowl (Numididae)) were collected, the viral RNA was extracted and RT-PCR carried out by QIAGEN one-step RT-PCR kit using the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates.Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran. According to our results, GCOVs are circulating in different avian species and further studies are needed to isolate these viruses and evaluate their pathogenesis.The source of wild bird infections with IBV-like CoV was probably poultry, and IBV genome detection could suggest that wild birds might constitute the mobile genome deposit of the CoV carrying gene pool, even in a huge geographic territory and Iran.

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