Restriction Fragment Length Polymorphism Typing of Staphylococcus aureus Strains Isolated from Bovine Mastitis and Dairy Products in Ahvaz, Iran, Using of Digested Coagulase Gene

Document Type: Short Communication

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization , Ahvaz, Iran

3 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Staphylococcus aureus is a major pathogen in the transmission of diseases from animals to humans and vice-versa.Various infections, such as mastitis in cattle, sheep and goats, as well as gastroenteritis due to food poisoning in humans are the most frequent problems caused by S. aureus. The bacteria also lead to severe economic losses in dairy industry. A major virulence factor for the organism is encoded by the coagulase (coa) gene. This study aimed to assess the polymorphisms of the coa gene in S. aureus strains isolated from bovine mastitis and dairy product samples in Ahvaz, Iran. The results showed that out of 91 S. aureus, 80 (87.91%) isolates were positive for coa gene(s). In total, nine different polymerase chain reaction (PCR) products were obtained for coa-positive isolates. A single band was detected in coa PCR with a size ranges from 370 to 830 bp in most isolates (n=77, 96.25%). For three isolates (3.75%), two amplification products were obtained. A PCR product of an estimated size of 590 bp was most frequent, as obtained for 48 (60.00%) isolates. Whereas, 370 and 830 bp PCR products were the least presented, for two (2.50%) and one (1.25%) isolate, respectively. Subsequently, for restriction fragment length polymorphism (RFLP), typing of coa gene and AluI restriction enzyme were used for the digestion of the products. AluI for most of PCR products generated a unique pattern; however, four PCR products (the sizes ranged 750, 670, 590, and 510 bp) generated three or more patterns. Based on AluI RFLP of coa gene, the isolates were classified into 23 groups. Two groups of isolates were dominant, making 45% of the total. According to the findings, one or two types of coa RFLP were dominant among samples that were infected with more S. aureus isolates belonging to different coa RFLP types.

Keywords

Main Subjects


Article Title [French]

Typage des souches de Staphylococcus aureus isolées à partir de mastites bovines et de produits laitiers à Ahvaz (Iran), en utilisant le polymorphisme de la longueur des fragments de restriction du gène de la coagulase

Abstract [French]

Staphylococcus aureus est un agent pathogène majeur dans la transmission de maladies d'animaux à l'homme et vice versa. Diverses infections, telles que les mammites chez les bétails, moutons et chèvres, ainsi que les gastro-entérites dues à une intoxication alimentaire chez l'homme, sont les problèmes les plus fréquemment causés par S. aureus. La bactérie entraîne également de graves pertes économiques dans l'industrie laitière. L’un des facteurs de virulence majeur pour ces microorganismes est codé par le gène de la coagulase (coa). Cette étude visait à évaluer les polymorphismes du gène coa dans les souches de S. aureus isolées à partir d'échantillons de mammites bovines et de produits laitiers à Ahvaz, en Iran. Les résultats ont montré que 80 réaction en chaîne de la polymérase (PCR) ont été obtenus pour les isolats coa-positifs. Pour la plupart des isolats analysés, le produit de la PCR coa était une bande unique de 370 à 830 pb (n=77, 96,25%). Pour trois isolats (3,75%), deux produits d'amplification ont été obtenus. Un produit de PCR d'une taille estimée de 590 pb était le plus fréquent et a été obtenu pour 48 isolats (60,00%). Alors que les produits de PCR de 370 et 830 pb étaient les moins représentés, uniquement détectés dans deux (2,50%) et un (1,25%) isolats, respectivement. Ensuite, pour le polymorphisme de longueur des fragments de restriction (RFLP), le typage du gène coa et de l'enzyme de restriction AluI ont été utilisés pour la digestion des produits. Dans la plupart des produits de PCR, AluI a généré un modèle unique. Cependant, quatre produits de PCR (avec des tailles de 750, 670, 590 et 510 pb) ont généré trois ou plusieurs modèles. Sur la base de la RFLP AluI du gène coa, les isolats ont été classés en 23 groupes. Deux groupes d'isolats étaient dominants, représentant 45% du total. Selon les résultats, un ou deux types de RFLP coa étaient dominants dans les échantillons infectés par plusieurs isolats de S. aureus appartenant à différents types de RFLP coa.

Keywords [French]

  • Staphylococcus aureus
  • gène de la coagulase
  • Réaction en chaîne de la polymérase
  • polymorphisme de la longueur des fragments de restriction
  • mammite bovine
  • produits laitiers
  • Iran
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