Scolicidal activity of Mesobuthus eupeus venom against the protoscolices of Echinococcus granulosus

Document Type: Short Communication

Authors

1 Department of Venomous Animals and Toxins. Razi Vaccine and Serum Research Institute, Ahvaz, Agriculture Research, Education and Extension Organization, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Hydatidosis is an important zoonosis caused by a parasitic tapeworm, namely Echinococcus granulosus. This infection is distributed worldwide and affects the health as well as economic loss in both humans and animals. In most cases, the disease needs chemotherapy with or without surgery. Conventional drugs have some major problems, including drug complications, harmful side effects, and also progressive resistance. According to the importance of biological productions as alternative medicine, a large number of studies confirmed that whole venom and many peptide ingredients of the scorpion venom have various different medical benefits, including antimicrobial properties, due to the mechanism of blocking gated ion channel. In this study, the venom peptides of Mesobuthus eupeus scorpionwere purified using gel filtration chromatography and subsequently ion exchange chromatography, followed by the determination of the molecular weights of the proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) procedure. After collecting the hydatid cysts fluids from the liver of infected sheep, protoscolices were derived, washed, and encountered to the whole venom as well as eight different fractions of toxin 30, 60, 120, and 240 min after the exposure. In the next step, the viability of protoscolices was determined by eosin staining. The obtained results revealed that a venom fraction under 10 kDa killed all protoscolices after 30 min. Moreover, it was found that the scolicidal activity of fractions increases according to the time of exposure. As a result, it can be concluded that M. epeus venom peptides under its LD50 (1/2 LD50) can properly and quickly destroy the protoscolices of hydatid cysts at the level of applied concentrations and such components are good alternatives to treat hydatidosis.

Keywords

Main Subjects


Article Title [French]

Activité Scolicidal du Venin de Mesobuthus eupeus contre les Protoscolices d’Echinococcus granulosus

Abstract [French]

L'hydatidose est une zoonose importante causée par un ver solitaire parasite, à savoir Echinococcus granulosus. Cette infection est répandue dans le monde entier et affecte la santé humaine et animale, induisant des pertes économiques considérables. Dans la plupart des cas, la maladie nécessite une chimiothérapie avec ou sans chirurgie. Les médicaments classiques présentent des problèmes majeurs, notamment des complications médicamenteuses, des effets secondaires nocifs et une résistance progressive. Selon l'importance des productions biologiques en tant que médecine alternative, de nombreuses études ont confirmé que le venin entier ainsi que des nombreux ingrédients peptidiques du venin de scorpion présentaient divers avantages médicaux, y compris des propriétés antimicrobiennes, en raison du mécanisme de blocage des canaux ioniques. Dans cette étude, les peptides de venin de scorpion Mesobuthus eupeus ont été purifiés par une chromatographie sur gel de filtration suivie d’une chromatographie à échange d’ions, puis par détermination du poids moléculaire des protéines par électrophorèse sur gel de dodécyl sulfate de sodium et de polyacrylamide (SDS-PAGE). Après avoir recueilli les fluides de kystes hydatiques du foie de mouton infecté, les protoscolex ont été extraits, lavés et exposés auvenin entier ainsi qu’àhuit fractions différentes de toxine durant 30, 60, 120 et 240 min. Ensuite, la viabilité des protoscolex a été déterminée par coloration à l'éosine. Les résultats obtenus ont révélé qu'une fraction de venin de moins de 10 kDa avait éliminé tous les protoscolices après 30 min. De plus, il a été constaté que l’activité scolicide des fractions augmente en fonction du temps d’exposition. En conséquence, on peut en conclure que les peptides du venin de M. epeus avec sa DL50 (1/2 LD50) peuvent détruire correctement et rapidement les protoscolices des kystes hydatiques dans les concentrations appliquées dans cette étude et que de tels composants sont de bonnes alternatives pour traiter l’hydatidose.

Keywords [French]

  • Kyste hydatique
  • Protoscolex
  • Mesobuthus eupeus
  • Venin
  • Traitement
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