Prevalence of Canine Monocytic Ehrlichiosis in Canine Population Across India

Document Type: Original Articles

Authors

1 Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh

2 St. aloysius College, Jabalpur (MP), India

3 Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, 250001, India

Abstract

Canine ehrlichiosis is a very important emerging disease in India. This study is the first attempt screening a large number of canines in India for the detection of canine monocytic ehrlichiosis. In the present study, 510 blood samples of dogs were screened for the presence of Ehrlichia canis and other variants of Anaplasmataceae family by serological and molecular methods.Out of the 510 serum samples, 293 (57.5%) cases were found positive for the presence of E. canis antibodies through enzyme-linked immunosorbent assay (ELISA). Furthermore, and 45 (8.8%) and 1 (0.2%) specimens were positive for E. canis and A. platys, respectively, based on the polymerase chain reaction (PCR). In the clinical samples of E. canis, the minimum detection limit for PCR was9 ng. In the immunofluorescence assay (IFA), the positive blood samples showed comparable results with those obtained from the commercially available dot ELISA kit (giving equivalent IFA titer). The results of sequencing were compared with other reported isolates in various regions of the world, and a phylogenetic relationship was established. The 16S rRNA region that was amplified and sequenced for E. canis and A. platys was highly conserved and so was another Vir B9 region.

Keywords

Main Subjects


Article Title [French]

Étude de la prévalence de l'infection par l'érythème monocytaire canin chez des populations canines en Inde

Abstract [French]

L'ehrlichiose canine est une maladie émergente très importante en Inde. C'est la première fois en Inde qu'un si grand nombre de populations canines ont été examinées pour la détection de l'ehrlichiose monocytaire canine. Dans cette étude, environ 510 échantillons de sang de chiens ont été examinés pour la présence d'Ehrlichia canis et d'autres variantes de la famille Anaplasmataceae par des méthodes sérologiques et moléculaires. Sur 510 échantillons de sérum, 293 (57,5%) étaientpositifs et présentaientdes anticorps contre E. canis en ELISA, alors que seulement 45 (8.8%) échantillons étaient positifspour E. canis et 1 (0,2%) pour A. platys en PCR. Dans les échantillons cliniques d’E. canis, la limite de détection minimale pour la PCR était de 9 ng. Dans le test IFA, les échantillons de sang positifs traités ont montré des résultats comparables avec le kit ELISA dot disponible dans le commerce donnant un titre d'IFA équivalent. Les résultats du séquençage ont été comparés avec d'autres isolats signalés dans diverses régions du monde et une relation phylogénétique a été établie. La région de l'ARNr 16S qui a été amplifiée et séquencée pour E. canis et A. platys s'est avérée être hautement conservée, tout comme une autre région Vir B9.

Keywords [French]

  • Ehrlichiosis canis
  • Anaplasma platys
  • Polymerase Chain Reaction
  • ELISA
  • Inde
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