Molecular Detection of Mycoplasma synoviae from Backyard and Commercial Turkeys in Some Parts of Iran

Document Type : Original Articles

Authors

1 Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

Abstract

M. synoviae (MS) is an economically important pathogen and the major cause of airsacculitis and infectious synovitis in turkeys. Infection with this pathogen may remain asymptomatic but can render infected birds susceptible to secondary infections. This study was carried out for the molecular detection of MS infection in commercial and backyard turkey flocks in Tehran, Semnan, Isfahan, Qazvin, Zanjan, East Azerbaijan, Gilan, Mazandaran, and Golestan provinces of Iran. Sixty-hundred tracheal, choanal cleft or/and infraorbital sinus samples were collected from 18 commercial and 31 backyard turkey flocks. The polymerase chain reaction (PCR) technique was performed by using primers specific for detecting the 16S rRNA and vlhA genes of MS. The results showed that 51.61% of backyard and 33.33% of commercial farms were MS-positive. These findings suggested the molecular presence of MS, especially in northern and central regions of Iran. Further, the frequency of MS-positive samples was significantly lower in commercial farms than backyard farms (P<0.05).

Keywords

Main Subjects


Article Title [French]

Détection moléculaire de Mycoplasma synoviae chez des dindes domestiques et commerciales dans certaines parties de l'Iran

Abstract [French]

Mycoplasma synoviae (MS) est un agent pathogène important sur le plan économique et la principale cause de l'airsacculite et de la synovite infectieuse chez les dindons. L'infection peut rester sans signes cliniques, mais peut rendre les oiseaux sensibles aux infections secondaires. Cette étude a été réalisée pour la détection moléculaire de l'infectionau Mycoplasma synoviae (MS) dans les fermes commerciales de dindes dans les provinces iraniennes de Téhéran, Semnan, Ispahan, Qazvin, Zanjan, Azerbaïdjan oriental, Gilan, Mazandaran et Golestan. Un total de 600 échantillons de trachée, de fente choanale et / ou de sinus infra orbital ont été prélevés à partir de 18 troupeaux de dindes commerciales et 31 dindes de basse-cour. La réaction en chaîne par polymérase (PCR) a été réalisée avec des amorces spécifiques pour la détection des gènes ARNr 16S et vlhA de la SEP. Les résultats ont été comparés et ont montré que 51,61% des basses-cours et %33,33 des fermes commerciales étaient séropositives. Ces résultats suggèrent la présence moléculaire du MS, en particulier dans le nord et le centre de l'Iran. De même que les échantillons MS positifs obtenus des fermes commerciales étaient significativement plus bas que dans les basses cours (0/05

Keywords [French]

  • Mycoplasma synoviae
  • Commercial
  • Basse-cour
  • Turquie
  • Détection Moléculaire
  • Iran
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