Distribution of Antibiotic Resistance Genes among the Phylogroups of Escherichia coli in Diarrheic Calves and Chickens Affected by Colibacillosis in Tehran, Iran

Document Type: Short Communication

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

2 Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Giuseppe Caporale, Department of Research and Development, Campo Boario, 64100 Teramo TE, Italy

3 Department of Microbiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

4 Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

5 Department of Veterinary Parasitology

Abstract

Antibiotic resistance occurs in the endogenous flora of exposed population in addition to pathogenic bacteria. This study was conducted to evaluate the distribution of antibiotic resistance genes among 63 isolates of Escherichia coli of Escherichia coli (E. coli) in diarrheic calves and poultry. According to the results, B1 and B2 were the most prevalent phylogroups of E. coli in calves and poultry carcasses, respectively. Antimicrobial resistance was observed in 76% of the isolates, and 62% of the strains were multi-drug resistant. Antibiotic resistance in E. coli strains obtained from calves strains was significantly higher than those obtained from poultries. Additionally, the strains of B1 and D phylogroups had the highest and lowest antimicrobial resistance, respectively. At least one encoding gene for integrone was detected in 23 strains (36.5%) and Class I integron had the highest prevalence. Accordingly, this study gave baseline information on the magnitude of the resistance problem and its genetic background in E. coli from domesticated animals of the Tehran, Iran. Moreover, the power of oligonucleotide array technology in the discrimination of different genotypes during a short time was confirmed in this study.

Keywords

Main Subjects


Article Title [French]

Distribution des gènes de résistance aux antibiotiques parmi les phylogroupes d’Escherichia coli chez les veaux diarrhéiques et les poulets touchés par la colibacillose à Téhéran, Iran

Abstract [French]

La consommation d’antibiotiques peut induire une résistance non seulement dans la flore endogène mais également chez les bactérie pathogène. Ce travail de recherche a été mené pour évaluer la distribution des gènes de résistance parmi 63 phylogroupes d’Escherichia coli (E. coli) chez les veaux diarrhéiques et la volaille. Selon les résultats, B1 et B2 étaient respectivement les phylogroupes les plus répandus d’E. coli chez les veaux et les carcasses de volaille. Unerésistance antimicrobienne a été observée chez 76% des isolatset 62% des souches étaient multi-résistantes aux médicaments. La résistance aux antibiotiques chez les souches d’E. coli isoléesà partir des échantillons provenant des veaux était significativement plus élevée que celles obtenues à partir des volailles. De plus, les souches des phylogroupes B1 et D présentaient la résistance antimicrobienne la plus élevée et la plus faible. Au moins, un gène codant pour l’intégrone a été détecté en 23 souches (36.5%) et l’intégrone de Classe I montraitla prévalence la plus élevée. En conclusion, ce travail de recherche a fourni des informations de base sur l'ampleur du problème de résistance et son contexte génétique chez les souches d’E. coli isolées chez des animaux domestiques de Téhéran, Iran. De plus, l’efficacité de la technologie de réseau d'oligonucléotides dans la discrimination de différents génotypes surune courte durée a été confirmée dans cette recherche.

Keywords [French]

  • Escherichia coli
  • Intégrone
  • Gènes de résistance
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