Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique

Document Type: Short Communication

Authors

1 Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan

2 Department of Microbiology, Faculty of Life Sciences & Informatics Balochistan University of IT, Engineering & Management Sciences (BUITEMS), Quetta Takkatu Campus, Airport road Quetta, Balochistan, Pakistan

3 Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan

4 Department of Biosciences, Faculty of Science, Barrett Hodgson University, Karachi, Pakistan

Abstract

Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples.

Keywords

Main Subjects


Article Title [French]

Diagnostic des Mycoplasmes Aviaires: Comparaison entre la PCR et la Technique de Culture

Abstract [French]

Mycoplasma gallisepticum et Mycoplasma synoviae sont les agents responsables de la mycoplasmose aviaire chez les volailles commerciales. Parmi les outils disponibles, l'amplification en chaîne par polymérase (PCR) et la culture représentent des methodes de confirmation pour le diagnostic de la mycoplasmose après le dépistage sérologique initial des oiseaux suspectés. Au total, 181 échantillons ont été analysés, 152 (84%) et 103 (57%) se sont révélés positifs par ACP et culture, respectivement. De plus, 54 (92%) des échantillons de poulet de chair étaient positifs pour les mycoplasmes aviaires communs. Parmi les échantillons positifs détectés par PCR, 72 étaient positifs pour MS (47%), alors que ce nombre était réduit 45 (44%) pour les échantillons analysés par culture. La positivité du MG était respectivement de 23% et 25% dans les échantillons analysés par PCR et culture. MG semblait proliférer plus facilement que MS. La valeur de l'accord entre les tests était de 67%. Globalement, la prévalence évaluée dans les différents troupeaux n'était pas très variable. La prévalence de la mycoplasmose était plus élevée en hiver. Notre étude a confirmé que la PCR est l’outil le plus sensible et le plus fiable pour le diagnostic de la mycoplasmose aviaire dans les relevés de terrain.

Keywords [French]

  • Mycoplasmose Aviaire
  • Mycoplasma gallispticum
  • Mycoplasma synoviae
  • ACP
  • Culture
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