A Serological Survey of Neospora caninum Infection in Urban and Rural Dogs in Ahvaz District, Southwest of Iran

Document Type: Original Articles

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Graduate Student, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Dogs are important in the epidemiology of Neospora caninum because they act as definitive hosts, shedding oocysts in the environment. The aim of the present survey was to evaluate the serological prevalence of Neospora caninum infection in urban and rural dogs in Ahvaz district, southwest of Iran. In this study, blood samples were taken from 100 rural dogs and 50 urban dogs. The dogs were categorized into two age groups (i.e., ≤ 3 and > 3 years). Neospora agglutination test (NAT) was performed for the detection of infection. Among 150 samples, 30 (20%) showed infection in 1:50 to 1:800 dilutions by NAT (confidence interval 95%: 13.60-26.40). The antibody titers were as follows: 1:50 (n=1), 1:100 (n=14), 1:200 (n=3), 1:400 (n=10) and 1:800 (n=2). The highest serum dilution was 1:100 in 46.67% of the infected dogs and the lowest serum dilution was 1:50 in 3.33% of them. The obtained results showed a significant difference in seroprevalence between urban (10%) and rural (25%) dogs (P=0.03). Although the seroprevalence was higher in dogs above three years of age (23.33%) than below three years (17.78%), there was not a significant difference among different age groups in this regard (P>0.05). The possibility of infection in dogs above the age of three years was 1.3 more than those below three years of age (confidence interval 95%: 0.58-2.9). It can be concluded that a relatively considerable percentage of dogs in Ahvaz district are infected with N. caninum. These infected dogs can play an important role in the transmission of neosporosis to other animals.

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Main Subjects


Article Title [French]

Une étude sérologique sur l'infection au Neospora caninum chez les chiens urbains et ruraux dans le district d'Ahvaz, au sud-ouest de l'Iran

Abstract [French]

Les chiens jouent un rôle important dans l'épidémiologie du Neospora caninum, car ils agissent comme hôtes définitifs, excrétant des oocystes dans l'environnement. L'objectif de cette étude était d'évaluer la prévalence sérologique de l'infection au Neospora caninum chez les chiens urbains et ruraux dans le district d'Ahvaz, au sud-ouest de l'Iran. Dans cette étude, des échantillons de sang ont été prélevés chez 100 chiens en milieu rural et 50 chiens en milieu urbain. Les chiens ont été classés en deux groupes d'âge (i.e. ≤ 3 et> 3 ans). Le Test d'Agglutination du Neospora (TAN) a été réalisé pour la détection de l'infection. Parmi les 150 échantillons, 30 (20%) présentaient une infection dans des dilutions de 1:50 à 1: 800 par TAN (intervalle de confiance à 95%: 13,60-26,40). Les titres en anticorps étaient les suivants: 1:50 (n = 1), 1: 100 (n = 14), 1: 200 (n = 3), 1: 400 (n = 10) et 1: 800 (n = 2). Les dilutions de sérum les plus élevées et les plus faibles étaient de 1: 100 et 1:50 chez respectivment 46,67% et 3,33% des chiens infecéts. Les résultats obtenus ont montré une différence significative de séroprévalence entre les chiens urbains (10%) et ruraux (25%) (P = 0,03). Malgré le fait que la séroprévalence était plus élevée chez les chiens de plus de trois ans(23,33%) comparé à ceux de moins de trois ans (17,78%), il n’y avait pas de différence significative entre les différents groupes d’âge (p> 0,05). Cependant, la possibilité d'infection chez les chiens âgés de plus de trois ans était de 1,3 fois supérieure à celle des chiens de moins de trois ans (intervalle de confiance à 95%: 0,58-2,9). Pour conclure un pourcentage relativement considérable de chiens dans le district d'Ahvaz est infecté par N. caninum. Ces chiens infectés peuvent jouer un rôle important dans la transmission de la néosporose à d'autres animaux.

Keywords [French]

  • Neospora caninum
  • test d'agglutination Neospora (TAN)
  • séroprévalence
  • Ahvaz
  • Chien
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