Plasma Paraoxonase, Sphingosine-1-phosphate, Total Sialic Acid, and Heat Shock Protein-27 in the Liver of the Sheep Naturally Infected with Cysticercus Tenuicollis: Evidence on Pathological Changes

Document Type: Short Communication

Authors

1 Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran

2 Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

Abstract

The present study aimed to investigate whether sphingosine 1 phosphate (S1P), paraoxonase (PON), total sialic acid (TSA), and heat shock protein-27 (HSP27) are altered in the sheep during infection of the liver with Cysticercus tenuicollis. This study was conducted on40 healthy sheep and40 sheep with Cysticercus tenuicollis infection. The infected and non-infected animals were selected based on the observation of severe Cysticercus tenuicollis infection in the liver and absence of any hepatic cysts, respectively. All parameters were measured in serum and plasma. The results revealed a significant decrease (P<0.01) in PON, TSA, and albumin (Alb) in the infected group, compared with those in the healthy one. Furthermore, the infected sheep had a significant increase (P<0.01) in S1P, HSP-27, malondialdehyde (MDA), total bilirubin, and unconjugated bilirubin as compared with those in their non-infected counterparts. Moreover, no significant change was observed in total plasma protein level in the infected animals in comparison to that in the healthy ones. The low levels of TSA and Alb revealed liver damage in the infected sheep. Moreover, the PON reduction might have resulted from hepatic steatosis and MDA enhancement. Meanwhile, S1P elevation could be attributed to the activation of platelets. In addition, HSP-27 increase was ascribed to the disease-induced stress conditions.

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Main Subjects


Article Title [French]

Paraoxonase plasmatique, Sphingosine-1-phosphate, Acide sialique total et Protéine 27 de choc thermique chez les ovins atteints de Cysticercus taeniaculis hépatique naturellement infecté : preuves de changements pathologiques

Abstract [French]

Cette étude a pour but d’évaluer les modifications rencontrées dans le foie du mouton lors d'une infection naturelle de Cysticercus taeniaculis, notamment au niveau de la sphingosine 1 phosphate (S1P), la paraoxonase (PON), l'acide sialique total (TSA) et la protéine de choc thermique 27 (HSP-27) .Dans ce but, 40 moutons parasités et 40 moutons sains ont été respectivement sélectionnés sur la base de la présence de formes kystiques sévères dans le foie et de l'absence de parasite sanguin sans conformation kystique dans la carcasse. Tous les paramètres ont été mesurés et comparés dans le sérum et le plasma. les résultats ont révélé une diminution significative (p <0,01) du PON, du TSA, et de l'albumine (Alb) ainsi qu’une augmentation significative (p <0,01) du S1P, du HSP-27, du malondialdéhyde (MDA, aldéhyde malonique), de la bilirubine totale et de la bilirubine non-conjuguée dans le groupe infecté comparé aux groupes sains. Aucun changement considérable n’a été observé au niveau de la protéine plasmatique totale (TPP) dans le groupe de la cysticercose par rapport aux sujets sains. Les faibles niveaux de TSA et Alb chez les moutons atteints révèlent des dommages au foie. De plus, la réduction du PON pourrait être due à un changement du taux de graisse dans le foie et à une augmentation du MDA. Parallèlement, l'augmentation des taux de S1P et de HSP-27 chez les moutons contaminés peut être respectivement attribuée à l'activation des plaquettes et aux conditions de stress induites par la maladie.

Paraoxonase, Sphingosine-1-phosphate, Acide sialique total, Protéine de choc thermique-27, Cysticercus taeniaculisParaoxonase, Sphingosine-1-phosphate, Acide sialique total, Protéine de choc thermique-27, Cysticercus taeniaculis

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