Pretreatment with Ultrasonication Reduces the Size of Azelaic Acid-Chitosan Nanoparticles Prepared by Electrospray

Document Type: Original Articles

Authors

1 Nanobiotechology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Nowadays, electrospray is becoming a favourable approach for preparing monodispersed nanoparticles. However, this approach is quite recent and requires further works to optimize control over physicochemical properties of its products. This study aimed to determine the possible effects of sonication as a pretreatment to reduce the size of azelaic acid-chitosan particle before using electrospray. The results showed that sonication treatment can produce submicron particles of azelaic acid-chitosan. By diluting the solution and increasing sonication time and amplitude, smaller particles were obtained with the smallest one at 516 nm, sized by dynamic light scattering. The pretreated solution was then electrosprayed to reduce the size of nanoparticles to 80 nm, indicating that sonication may play an important role in reducing the size of electrosprayed nanoparticles. The electrosprayed nanoparticles were nearly monodispersed and almost spherical in shape.

Keywords

Main Subjects


Article Title [French]

Le prétraitement par ultrasonication réduit la taille des nanoparticules d'acide azélaïque-chitosane préparées par électrospray

Abstract [French]

L’electrospray est aujourd’hui une approche favorable pour la préparation de nanoparticules monodispersées. Cette méthodeest cependant assez récente et nécessite des travaux supplementaires afin demieux contrôler les propriétés physicochimiques des produits traités. Cette étude visait à déterminer les influences possibles d’un prétraitement par sonication sur la taille des particules issues d’une solution de chitosane-acide azélaïque. Les résultats ont montré que le traitement par ultrasons permet de produire des particules submicroniques d'acide azélaïque-chitosane. En diluant la solution et en augmentant le temps et l'amplitude de la sonication, des particules plus petites sontobtenues dont lesplus petites ont été mesurées par diffusion dynamique de la lumière à 516 nm. La solution prétraitée a ensuite été soumise à uneélectropulvérisation afin de diminuer la taille des nanoparticules jusqu'à 80 nm. Ceci montre que la sonication peut jouer un rôle important dans la réduction de la taille des nanoparticules traitées par électrospray. Les nanoparticules issues de l’électrospray étaient plus ou moins monodispersées et de forme sphérique.

Keywords [French]

  • Electrospray
  • le chitosane
  • l'acide azélaïque
  • ultrasonication
Arya, N., Chakraborty, S., Dube, N., Katti, D.S., 2009. Electrospraying: a facile technique for synthesis of chitosan‐based micro/nanospheres for drug delivery applications. Appl Biomater 88, 17-31.

Azra, Y., Linggar, S., Emma, S., Anita, R., 2012. The Effect of Sonication on the Characteristic of Chitosan. Proceeding of International Conference on Chemical and Material Engineering.

Bilati, U., Allémann, E., Doelker, E., Nanoprecipitation versus emulsion-based techniques for the encapsulation of proteins into biodegradable nanoparticles and process-related stability issues. AAPS Pharm Sci Tech 6, E594-E604.

Bilati, U., Allémann, E., Doelker, E., 2005. Development of a nanoprecipitation method intended for the entrapment of hydrophilic drugs into nanoparticles. Eu J Pharma Sci 24, 67-75.

Braghirolli, D.I., Zamboni, F., Chagastelles, P.C., Moura, D.J., Saffi, J., Henriques, J.A.P., et al., 2013. Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering. Biomicrofluidics 7, 044130.

Breathnach, A., Levi-Montalcini, R., 1995. The story of azelaic acid. A tribute to Marcella Nazzaro-Porro. Rend Fis Acc Lincei 6, 313-320.

Capelo-Martínez, J.L., 2009. Ultrasound in Chemistry: Analytical Applications, Wiley.

Cornils, B., Lappe, P., 2000. Dicarboxylic Acids, Aliphatic. Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH Verlag GmbH & Co. KGaA.

Enayati, M., Chang, M.-W., Bragman, F., Edirisinghe, M., Stride, E., 2011. Electrohydrodynamic preparation of particles, capsules and bubbles for biomedical engineering applications. Physicochem Engin Asp 382, 154-164.

Esmaeilzadeh-Gharedaghi, E., Faramarzi, M.A., Amini, M.A., Rouholamini Najafabadi, A., Rezayat, S.M., Amani, A., 2012. Effects of processing parameters on particle size of ultrasound prepared chitosan nanoparticles: an Artificial Neural Networks Study. Pharm Dev Technol 17, 638-647.

Esmaeilzadeh Gharehdaghi, E., Amani, A., Khoshayand, M.R., Banan, M., Esmaeilzadeh Gharehdaghi, E., Amini, M.A., et al., 2014. Chitosan nanoparticles for siRNA delivery: optimization of processing/formulation parameters. Nucleic Acid Ther 24, 420-427.

Fitton, A., Goa, K.L., 1991. Azelaic acid. A review of its pharmacological properties and therapeutic efficacy in acne and hyperpigmentary skin disorders. Drugs 41, 780-798.

Hwang, Y., Lee, J.-K., Lee, J.-K., Jeong, Y.-M., Cheong, S.-i., Ahn, Y.-C., et al., 2008. Production and dispersion stability of nanoparticles in nanofluids. Powder Technol 186, 145-153.

Jayakumar, R., Nwe, N., Tokura, S., Tamura, H., 2007. Sulfated chitin and chitosan as novel biomaterials. Int J Biol Macromol 40, 175-181.

Jimtaisong, A., Saewan, N., 2014. Use of Chitosan and its Derivatives in Cosmetics. Chemistry 9, 6.

Karimi Zarchi, A.A., Abbasi, S., Faramarzi, M.A., Gilani, K., Ghazi-Khansari, M., Amani, A., 2015. Development and optimization of N-Acetylcysteine-loaded poly (lactic-co-glycolic acid) nanoparticles by electrospray. Int J Biol Macromol 72, 764-770.

Kumar, M.N., Muzzarelli, R.A., Muzzarelli, C., Sashiwa, H., Domb, A.J., 2004. Chitosan chemistry and pharmaceutical perspectives. Chemical reviews 104, 6017-6084.

Kumari, A., Yadav, S.K., Yadav, S.C., 2010. Biodegradable polymeric nanoparticles based drug delivery systems. Biointerfaces 75, 1-18.

Lassalle, V., Ferreira, M.L., 2007. PLA Nano- and Microparticles for Drug Delivery: An Overview of the Methods of Preparation. Macromol Biosci 7, 767-783.

Miller, D., Smith, N., Bailey, M., Czarnota, G., Hynynen, K., Makin, I., et al., 2012. Overview of Therapeutic Ultrasound Applications and Safety Considerations. J Ultrasound Med 31, 623-634.

Patel, A.K., Michaud, P., Petit, E., de Baynast, H., Grédiac, M., Mathias, J.D., 2013. Development of a chitosan‐based adhesive. Application to wood bonding. J Appl Poly Sci 127, 5014-5021.

Pitt, W.G., Husseini, G.A., Staples, B.J., 2004. Ultrasonic Drug Delivery – A General Review. Expert Opin Drug Deliv 1, 37-56.

Raja Azalea, D., Mohambed, M., Joji, S., Sankar, C., B, M., 2012. Design and Evaluation of Chitosan Nanoparticles as Novel Drug Carriers for the Delivery of Donepezil. Iranian J Pharma Sci 8, 155-164.

Saha, P., Goyal, A.K., Rath, G., 2010. Formulation and evaluation of chitosan-based ampicillin trihydrate nanoparticles. Trop J Pharma Res 9.

Sridhar, R., Ramakrishna, S., 2013. Electrosprayed nanoparticles for drug delivery and pharmaceutical applications. Biomatter 3.

Suh, J.K., Matthew, H.W., 2000. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 21, 2589-2598.

Tang, E.S., Huang, M., Lim, L.Y., 2003. Ultrasonication of chitosan and chitosan nanoparticles. Int J Pharm 265, 103-114.

Valo, H., Peltonen, L., Vehviläinen, S., Karjalainen, M., Kostiainen, R., Laaksonen, T., et al., 2009. Electrospray Encapsulation of Hydrophilic and Hydrophobic Drugs in Poly (L-lactic acid) Nanoparticles. Small 5, 1791-1798.

Vehring, R., 2007. Pharmaceutical Particle Engineering via Spray Drying. Pharma Res 25, 999-1022.

Yeo, L.Y., Gagnon, Z., Chang, H.-C., 2005. AC electrospray biomaterials synthesis. Biomaterials 26, 6122-6128.

Zambaux, M.F., Bonneaux, F., Gref, R., Maincent, P., Dellacherie, E., Alonso, M.J., et al., 1998. Influence of experimental parameters on the characteristics of poly (lactic acid) nanoparticles prepared by a double emulsion method. J Control Release 50, 31-40.

Zarrabi, A., Vossoughi, M., 2009. Electrospray: Novel Fabrication Method for Biodegradable Polymeric Nanoparticles for Further Applications in Drug Delivery Systems. Roznov pod Radhostem 10, 20-22.

Zhang, S., Kawakami, K., 2010. One-step preparation of chitosan solid nanoparticles by electrospray deposition. Int J Pharm 397, 211-217.