Prevalence of Neospora caninum and Toxoplasma gondii Antibodies in Bulk Milk of Dairy Cattle, Mashhad, Iran

Document Type: Original Articles

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Neospora caninum (N. caninum) and Toxoplasma gondii (T. gondii) are both obligate intracellular protozoan parasites, which have gained considerable attention because of their role in bovine abortion. This study aimed to detect anti-N. caninum and -T. gondii in bulk milk of dairy cattle, Mashhad, Iran. The bulk milk samples were collected from July 2014 to June 2015 and analyzed for anti-N. caninum and -T.gondii antibodies using enzyme-linked immunosorbent assay (ELISA). Out of 123 bulk milk samples, 44 (35%), 14 (11.38%), and 3 (2.4%) samples had N. caninum, T. gondii, and mixed infection with these two parasites, respectively. According to the results, the prevalence of N. caninum infection was more than T.gondii infection in dairy cattle of Mashhad, Iran.

Keywords

Main Subjects


Article Title [French]

Estimation de la prévalence des anticorps dirigés contre les antigènes de Neospora caninum et Toxoplasma gondii dans le lait des réservoirs des fermes laitières dela ville de Mechhed

Abstract [French]

Neospora caninum et Toxoplasma gondii sont deux parents protozoaires intracellulaires constituant un risque sanitaire majeur en raison desavortementsqu’ils peuvent engendrés chez les vaches laitières. Cette étude avait pour objectif la détection des anticorps spécifiques aux antigènes de Neospora caninum et Toxoplasma gondii dans le laitprovenant des réservoirs des fermes laitières dans la ville de Mechhed en Iran. Pour ce faire des prélèvements de lait ont été effectués à partir desréservoirsdes fermes laitières de Mechhed entre juillet 2014 et juin 2015 et ont été soumis à un test d'ELISA afin de détecter les anticorps spécifiques aux antigènes de Neospora et de Toxoplasma. Sur un total de 123 prélèvements , 44 (35%), 14 (11,38%) et 3 (2,4%)échantillons étaient respectivement positifs au Neospora caninum, auToxoplasma gondii etaux deux espèces simultanément . Nos résultats ont révélé untaux d'infection plus important auxNeospora comparé auxToxoplasma dans les fermes laitières de la ville de Mechhed. Ces résultats ont démontré que le test ELISAsur des échantillons provenant des réservoirs de lait peut être utilisé en tant qu’un test quantitatif approprié afin d’estimer le taux dinfection auxNeospora et Toxoplasma dans les fermes laitières.

Keywords [French]

  • Lait de réservoir
  • test ELISA
  • Neospora caninum
  • Toxoplasma gondii
  • vache
  • ville de Mechhed

Bartels, C., Arnaiz-Seco, J., Ruiz-Santa-Quitera, A., Björkman, C., Frössling, J., Von Blumröder, D., et al., 2006. Supranational comparison of Neospora caninum seroprevalences in cattle in Germany, The Netherlands, Spain and Sweden. Vet Parasitol 137, 17-27.

Bartels, C., Van Maanen, C., Van der Meulen, A., Dijkstra, T., Wouda, W., 2005. Evaluation of three enzyme-linked immunosorbent assays for detection of antibodies to Neospora caninum in bulk milk. Vet Parasitol 131, 235-246.

Bártová, E., Sedlák, K., Budíková, M., 2015. A study of Neospora caninum and Toxoplasma gondii antibody seroprevalence in healthy cattle in the Czech Republic. Ann Agric Environ Med 22.

Belluco, S., Mancin, M., Conficoni, D., Simonato, G., Pietrobelli, M., Ricci, A., 2016. Investigating the determinants of Toxoplasma gondii prevalence in meat: a systematic review and meta-regression. PloS one 11, e0153856.

Björkman, C., Holmdahl, O.J.M., Uggla, A., 1997. An indirect enzyme-linked immunoassay (ELISA) for demonstration of antibodies to Neospora caninum in serum and milk of cattle. Vet Parasitol 68, 251-260.

Boughattas, S., 2017. Toxoplasma infection and milk consumption: Meta-analysis of assumptions and evidences. Crit Rev Food Sci Nutr 57, 2924-2933.

Chanlun, A., Näslund, K., Aiumlamai, S., Björkman, C., 2002. Use of bulk milk for detection of Neospora caninum infection in dairy herds in Thailand. Vet Parasitol 110, 35-44.

Dubey, J., 1986. A review of toxoplasmosis in cattle. Veterinary parasitology 22, 177-202.

Dubey, J., Schares, G., 2011. Neosporosis in animals—the last five years. Vet Parasitol 180, 90-108.

Dubey, J., Schares, G., Ortega-Mora, L., 2007. Epidemiology and control of neosporosis and Neospora caninum. Clin  Microbio  Rev 20, 323-367.

Frössling, J., Nødtvedt, A., Lindberg, A., Björkman, C., 2008. Spatial analysis of Neospora caninum distribution in dairy cattle from Sweden. Geospat Health 3, 39-45.

Gharekhani, J., Haddadzadeh, H., Bahonar, A., 2014. Prevalence of immunoglobulin G (IgG) antibody to Neospora caninum in dairy cattle of Hamedan province, west of Iran. Veterinary research forum: an international quarterly journal. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran, p. 149.

Gondim, L.F.P., Sartor, I., Hasegawa, M., Yamane, I., 1999. Seroprevalence of Neospora caninum in dairy cattle in Bahia, Brazil. Vet Parasitol 86, 71-75.

González-Warleta, M., Castro-Hermida, J.A., Carro-Corral, C., Cortizo-Mella, J., Mezo, M., 2008. Epidemiology of neosporosis in dairy cattle in Galicia (NW Spain). Parasitol Res 102, 243-249.

González-Warleta, M., Castro-Hermida, J.A., Carro-Corral, C., Mezo, M., 2011. Anti-Neospora caninum antibodies in milk in relation to production losses in dairy cattle. Prev Vet Med 101, 58-64.

Gottstein, B., Hentrich, B., Wyss, R., Thür, B., Busato, A., Stärk, K., et al., 1998. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. Int J Parasitol 28, 679-691.

Huong, L., Ljungström, B.-L., Uggla, A., Björkman, C., 1998. Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle and water buffaloes in southern Vietnam. Veterinary Parasitol 75, 53-57.

Hurkova, L., Halova, D., Modry, D., 2005. The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the Czech Republic: a case report. Vet Med (Praha) - 50, 549.

Klevar, S., Norström, M., Tharaldsen, J., Clausen, T., Björkman, C., 2010. The prevalence and spatial clustering of Neospora caninum in dairy herds in Norway. Vet Parasitol 170, 153-157.

Nasir, A., Lanyon, S.R., Schares, G., Anderson, M.L., Reichel, M.P., 2012. Sero-prevalence of Neospora caninum and Besnoitia besnoiti in South Australian beef and dairy cattle. Vet Parasitol 186, 480-485.

Nematollahi, A., Jaafari, R., Moghaddam, G., 2011. Seroprevalence of Neospora caninum infection in dairy cattle in Tabriz, Northwest Iran. Iran J Parasitol 6, 95.

O’Doherty, E., Berry, D.P., O’Grady, L., Sayers, R., 2014. Management practices as risk factors for the presence of bulk milk antibodies to Salmonella, Neospora caninum and Leptospira interrogans serovar hardjo in Irish dairy herds. animal 8, 1010-1019.