Detection of Mycoplasma agalactiae in Small Ruminants of Southeast Iran

Document Type: Original Articles

Authors

1 Department of Molecular Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Kerman, Iran

2 Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

Abstract

Agalactia is an infectious and contagious disease of small ruminants caused by Mycoplasma agalactiae (M. agalactiae). Although different microorganism strains contribute to this disease,  M. agalactiae is known as the most prominent causative agent. Therefore, this study aimed to investigate the rate of M. agalactiae involvement in contagious agalactia in the southeast region of Iran. Sampling was performed from milk, conjunctiva, ear lesions, and joints exudate of suspicious sheep and goat flocks according to the reports of Iran Veterinary Organization. The presence of Mycoplasma and its species, namely M. agalactiae, was evaluated through microbial culture and polymerase chain reaction (PCR) techniques. The detected microorganisms were confirmed to be Mycoplasma and M. agalactiae by the PCR amplification of 16S rRNA and lipoprotein target genes. According to the findings of present study, 14.8% and 36.0% of the samples were diagnosed as positive for Mycoplasma by culture and PCR, respectively. Moreover, the incidence of M. agalactiae was determined as 6.1% using the specific PCR method. Therefore, it is recommended to identify the other species of Mycoplasma in small ruminant samples involved with contagious agalactiae disease.

Keywords

Main Subjects


Article Title [French]

Détection de Mycoplasma agalactiae chez les petits ruminants du le Sud-est de l'Iran

Abstract [French]

L’agalaxie est une maladie infectieuse et contagieuse chez les petits ruminants engendrée notamment par le Mycoplasmaagalactiae (M. agalactiae). Différents facteurs contribuent à l’apparition del’agalaxie , mais M. agalactiae a été reconnu comme l'un des agents étiologiques les plus importants. Dans cetteétude, l'incidence de M. agalactiaeà l‘origine de l'agalaxie contagieuse a été évaluéedans le Sud-est de l'Iran. Des prélèvements ont été menés en respectant les consignes de l'organisation vétérinaire iranienne sur le lait, la conjonctive, leslésionsauculairesainsi que sur la la ponction du liquide synovial des troupeaux de brebis et chèvres suspectés d’être contaminés. La présence de Mycoplasma et de M. agalactiae a été détectée en utilisant les techniques de culture microbienne et de PCR. Mycoplasma et M. agalactiae ont été identifiés par PCR via l’amplification de la séquence 16S-rRNA desgènes cibles et par la caractérisation des lipoprotéines. Le taux de contamination par le genre Mycoplasmaa été respectivement estimé a14.8% et 36.0% par culture et par PCR. Les infections causées par M. agalactiaeconstituées 6.1% des échantillons. Cette étude souligne l’importance d'identifier d'autres espèces de Mycoplasma dans les échantillons de petits ruminants engagé avec maladie de l’agalaxie contagieuse.

Keywords [French]

  • Agalaxie
  • Mycoplasma agalactiae
  • Culture
  • PCR
  • Sud-est de l'Iran

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