Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin

Document Type: Original Articles


1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran

2 School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran

3 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran

4 Drug Applied Research Center, Tabriz University of Medical Sciences


Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.


Main Subjects

Article Title [French]

Étude comparative de la cytotoxicité et de l'activité antioxydante d’un extrait de pépins de raisin, de la crocine et de la phénytoïne

Abstract [French]

L’objectif de cette étude était d’évaluer et de comparer la capacité antioxydante et la cytotoxicité des extraits de pépins de raisin (GSE), de crocine (CRO) et de phénytoïne (PHEN) sur les cellules cancéreuses du sein. Les extraits de méthanol (MeOH) de ces composés ont été préparés et l'activité antioxydante de ces composés a été mesurée à l'aide de la méthode DPPH en utilisant de la quercétine (QUER) comme témoin positif. Le test a été utilisé pour l'évaluation de la cytotoxicité de ces différents composés sur des lignées cellulaires cancéreuses MCF-7 (cellules cancéreuses du sein). Dans cette expérience la doxorubicine (DOX) a été utilisée comme témoin positif. Les résultats de cette étude ont montré que l'activité anti-oxydante et la cytotoxicité de GSE sont plus élevées que CRO et PHEN, ainsi qu'il existe un lien direct entre l'activité anti-oxydante et la cytotoxicité de ces composés. Étant donné l'activité antioxydante et la cytotoxicité de GSE contre les cellules cancéreuses, cet extrait pourrait probablement accompagner d'autres composés dans le traitement du cancer. Des études supplémentaires sont nécessaires pour compléter ces résultats.

Keywords [French]

  • extrait de pépins de raisin
  • crocine
  • phénytoïne
  • cytotoxicité
  • activité antioxydante
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