Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran

Document Type : Short Communication

Authors

1 Department of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Pathobiology, Faculty of Veterinary Science, Tehran University, Tehran, Iran

4 Department of Microbiology, Faculty of Specialized Veterinary Science, Branch of Science and Research, Islamic Azad University, Tehran, Iran

Abstract

Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect T.annulata DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for Theileria spp. and T.orientalis amplification, respectively. The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both T.annulata and T.orientalis. Out of 291 buffaloes, four samples (1.4%) were infected by Theileria genotypes, and two buffaloes (0.7%) were infected only by T.orientalis. The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that T.orientalis may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with T.orientalis for the first time.

Keywords

Main Subjects

Article Title [French]

Diagnostic moléculaire et microscopique de Theileria orientalis chez les vaches de la province de l’Azerbaïdjan occidental, Iran

Abstract [French]

La theilériose bovine est une maladie importante causée par une cellule intra-érythrocytaire de Theileria. L’objectif de cette étude est de déterminer à l’aide de méthodes moléculaires et microscopiques l'état actuel de l’infection de Theileria chez les vaches de la province de l'Azerbaïdjan occidental en Iran. Dans cette étude, 484 échantillons de sang ont été prélevés entre mars et juillet 2014 à partir de vaches et buffles indigènes provenant de quatre villes différentes. Les animaux étudiés avaient été classés selon la race, le sexe, l’âge et la ville. Tous les échantillons sanguins des animaux étudués ont fait l’objet d’une coloration de Giemsa pour les observations parasitologiques . Les techniques de PCR et semi-nested PCR ont été également utilisées pour détecter l'ADN de Theileria orientalis à l’aide des paires d’ARN ribosomiques 18S: les ensemble d’amorces Tbs-S/Tbs-A et To/Tbs-A ont été respectivement utilisés pour l’amplification de Theileiria, et Theileria orientalis. . Les analyses moléculaires sur 484 échantillons ont détecté 40 échantillons infectés par le Theileria (8,26%) et 17 (3,51%) échantillons positifs pour le Theileria orientalis. La prévalence de Theileria orientalis chez les vaches indigènes et les buffles était respectivement de 8.33% et 0.68%, révélant pour la première fois une différence significative entre ces deux groupes (p<0.05). L’utilisation de cette approche moléculaire a confirmé l’existence d’infection au Theileria orientalis chez les vaches indigènes ainsi que chez certains buffles originaires plus particulièrement du nord-ouest de l'Iran.

Keywords [French]

  • Theileria orientalis
  • buffles
  • vaches
  • réaction en chaîne de polymérase
  • Iran

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Archives of Razi Institute
Volume 72, Issue 3
September and October 2017
Pages 189-195

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