Characterization of reoviruses isolated from some broiler breeder flocks in Iran

Document Type: Original Articles

Authors

1 Department of Animal Sciences, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran & Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Avian reoviruses (ARVs) are considered as an important cause of several diseases in poultry, particularly
arthritis and tenosynovitis. Tenosynovitis and arthritis, which are among the causes of chronic lameness in
breeder flocks, can result in reduced egg production and culling of breeder hens. In this study, the molecular
characteristics of ARVs in some broiler breeder flocks were investigated in Iran. After RNA extraction of the
field samples, reverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify two regions
of ARVs for S1 and S4 genes. The positive samples were further analyzed by five restriction enzymes in
restriction fragment length polymorphism (RFLP) for determining the strains. The phylogenetic analysis of S1
and S4 genes from the isolates indicated divergence into five and four major lineages, respectively. The
sequence analysis of S1 and S4 genes of ARVs revealed that most of the positive samples were closely related to
tenosynovitis-inducing ARVs (with less than 2% nucleotide divergence). Also, these samples were most
homologous to S1133 strain, with 99.90% nucleotide and amino acid affinity.

Keywords

Main Subjects


Article Title [French]

Caractérisation des réovirus isolés à partir de poules pondeusesen Iran

Abstract [French]

Les réovirus aviaires (ARVs) sont considérés comme une cause importante de maladies chez les volailles, particulièrement d’arthrite et de ténosynovite. Ces deux maladies sont à l’origine de boiteries chroniques chez les poules pondeuses,pouvant engendrer une réduction de la ponte et l’abattage des poules reproductrices. L’objectif de cette étude était la caractérisation moléculaire des ARVs affectant les poules pondeuses en Iran.Après l’extraction des ARN à partir des échantillons de terrain, une amplification en chaîne par polymérasetranscriptase inverse (RT-PCR) a été menée sur deux régions des ARVs, incluant les gènes S1 et S4. Les échantillons positifs ont été ensuite soumis à une analyse plus approfondie du polymorphisme de taille des fragments de restriction (PTFR) par 5 enzymes,afin de déterminer les souches impliquées. L’analyse phylogénétique des gènes S1 et S4 des isolats montre une divergence dans cinq et quatre grandes lignées, respectivement. L’analyse des séquences des gènes S1 et S4 a démontré que la majorité des échantillons positifs étaient étroitement liés aux ARVs induisant des ténosynovites (avec moins de 2% de divergence au niveau des nucléotides). De plus, ces échantillons étaient fortement homologues à la souche S1133, montrant une similitude nucléotidique et d’acides aminés de 99,90%.

Keywords [French]

  • caractéristiques moléculaires
  • Réovirus aviaires
  • Ténosynovite
  • Poules pondeuse
  • Iran

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