Prevalence and genotyping of Giardia duodenalis among Arabian horses in Ahvaz, southwest of Iran

Document Type: Original Articles

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Giardia duodenalis is globally recognized as an important zoonotic intestinal protozoan parasite. So far, eight assemblages of G. duodenalis (A-H) have been identified. Substantial evidence suggests the zoonotic potential of assemblages A, B, and E in livestock. In this study, the genotype of Giardia duodenalis isolates was genetically identified by determining the sequence of ssu-rRNA gene and performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on glutamate dehydrogenase gene of the parasite in Arabian horses from Ahvaz, located in southwest of Iran. The results revealed that assemblages AI and E (livestock-associated G. duodenalis) were present in horse isolates. Also, based on the findings, prevalence of G. duodenalis infection among horses was estimated at 35.7%. The results indicated that G. duodenalis is highly prevalent among Arabian horses, posing a zoonotic risk for giardiasis in Ahvaz, Iran.

Keywords

Main Subjects


Article Title [French]

Prévalence et génotypage de Giardia duodenalis chez les chevaux arabes d’Ahvaz, au sud-ouest de l’Iran

Abstract [French]

Giardia duodenalis est reconnu mondialement comme un parasite intestinal protozoaire zoonotique. Jusqu’alors, huit assemblages (A-H) ont été identifies pour l’espèce G. duodenalis. Des preuves substantielles laissent supposer un potentiel zoonotique chez les animaux d’élevages pour les assemblages A, B et E. Dans cette étude, les génotypes des isolats de Giardia duodenalis ont été génétiquement identifiés. Pour ceci, la séquence ssu-ARNr a été déterminée par polymorphisme de longueur des fragments de restriction (PCR-RFLP) sur le gène du glutamate déshydrogénase des parasites isolés à partir des chevaux arabes (Ahvaz, sud-ouest de l’Iran). Nos résultats révèlent que les assemblages AI et E (G. duodenalis associé à l’élevage) étaient présents dans les isolats des chevaux analysés. De plus, la prévalence de cette infection a été estimée à 35,7%. Ces données reflètent la forte prévalence du parasite G. duodenalis parmi les chevaux arabes, constituant de ce fait un risque zoonotique majeur de giardiase dans la région d’Ahvaz en Iran.

Keywords [French]

  • Giardia duodenalis
  • Génotypage
  • ssu-ARNr
  • gdh
  • Cheval arabe
  • Iran
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