Isolation, identification, and monitoring of antibiotic resistance in Pasteurella multocida and Mannheimia haemolytica isolated from sheep in East Azerbaijan province, Iran

Document Type: Original Articles

Authors

1 Virus Biobank Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran

2 Departent ofm Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran Department of Research and Development, Razi Vaccine and Serum Research Institute, Karaj, Iran

3 Department of Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran

4 Department of Research and Development, Razi Vaccine and Serum Research Institute, Karaj, Iran

5 Technical Director of Veterinary Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

The present study was carried out in order to isolate, identify, and assess the antimicrobial susceptibility of the causative agent(s) of pneumonic pasteurellosis in sheep in East Azerbaijan province, northwest of Iran. Pneumonia was detected in 320 cases, and the affected lungs were sampled in the slaughterhouse. The samples were investigated bacteriologically for the isolation of two microorganisms from the Pasteurellaceae family. Pasteurella multocida was isolated from six (1.87%) samples, while none of the lung tissues were positive for Mannheimia haemolytica. After the isolation and detection of microorganisms via cultural and morphological tests, the bacteria were identified on the basis of biochemical criteria and polymerase chain reaction (PCR) technique. Antimicrobial susceptibility testing was performed on all P. multocida isolates, using broth microdilution method. Evaluation of the minimum inhibitory concentration (MIC) of eight antimicrobial agents against the tested isolates showed that all the organisms were resistant to amoxicillin and relatively susceptible to ceftiofur. In conclusion, P. multocida was introduced as the main cause of ovine pneumonic pasteurellosis in the studied district, and the outbreak frequency significantly varied in different seasons of the year (P<0.05).

Main Subjects


Article Title [French]

Isolation, identification et contrôle de l’antibiorésistance des souches de Pasteurella multocida et Mannheimia haemolytica isolées à partir de moutons de l’Est Azarbaijan, Iran

Abstract [French]

L’objectif de cette étude était d’isoler, d’identifier et d’évaluer la susceptibilité antimicrobienne des agents responsables de la pasteurellose pneumonique chez les moutons de l’Est Azerbaïdjan, situé au nord ouest de l’Iran. Trois cent vingt (320) cas de pneumonie ont été détectés et le prélèvement des poumons affectés a été mené dans un abattoir. Tous les échantillons ont été ensuite soumis à des analyses bactériologiques et l’incidence de deux microorganismes spécifiques appartenant à la famille Pasteurellaceae a été étudiée. Selon nos analyses, six prélèvements pulmonaires étaient contaminés par Pasteurella multocida (1,87%), alors qu’aucun échantillon positif à la souche Mannheimia haemolytica n’a été détecté. Après l’isolation et la détection des microorganismes par des testes de culture et d’observation morphologiques, les souches bactériennes ont été identifiées grâce à leurs propriétés biochimiques et la méthode de réaction en chaîne par polymérase (PCR). La susceptibilité antimicrobienne de tous les isolats de P. multocida a été évaluée par la méthode de microdilution du bouillon de culture. L’évaluation de la concentration inhibitrice minimum (MIC) de 8 agents antimicrobiens vis-à-vis des isolats identifiés a révélé une résistance à l’ amoxicilline et une susceptibilité relative au ceftiofur. En conclusion, P. multocida se présente comme la cause majeure de pasteurellose pneumonique ovine dans la province de l’Est Azerbaïdjan et la fréquence cette épidémie varie de façon significative d’une saison à l’autre (P<0.05).

Keywords [French]

  • Isolation
  • Pasteurella multocida
  • Mannheimia haemolytica
  • Mouton
  • PCR
  • Résistance antimicrobienne
  • Iran

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