Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901Analysis of variations, structures, and phylogenic characteristics of bovine leukocyte antigen DRB3 exon2آنالیز تغییرات، ساختار و خصوصیات فیلوژنیک اگزون 2 از BoLA-DRB3 درگاو14715711161110.22092/ari.2017.111611ENM. M. RanjbarDepartment of Viral Animal Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran0000-0001-8611-5762S. AtaeiDepartment of Avian Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education
and Extension Organization, Karaj, Iran0000-0002-3929-3889Gh. Nikbakht BrujeniDepartment of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran0000-0002-2970-5827S. GolabdarDepartment of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranJournal Article20160529Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex<br />(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular and<br />evolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.<br />Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustrate some novel<br />practical evidence on interspecies diversity, the resistance /susceptibility points in cattle breeding, and vaccine<br />design. Initially, BoLA-DRB3.2* alleles and orthologous exons in the selected livestock were retrieved and<br />checked. In the next step, the secondary/tertiary structure of BoLA-DRB3.2*24 gene product was modeled and<br />validated. Then, hypervariable regions (HVRs) of alleles were identified by hybrid approaches. In the last step,<br />interspecies relationship, allele’s phylogeny/grouping, and estimate of average evolutionary divergence were<br />explored. Shannon entropy variation analysis showed eight HVRs and three semi-variable regions in BoLADRB3.2*<br />alleles. These HVRs were present in all the three sub-structures and dominantly existed in alpha helix.<br />In addition, strong relationships and little diversity were noted in phylogenetic trees of cattle, buffaloes, sheep,<br />and goats. Furthermore, there was some evidence on divergence of DRB3 before speciation among the<br />mentioned species and possibility of cross prediction resistance/susceptibility alleles. Finally, DRB3 alleles were<br />grouped into seven clusters, and older and newer alleles were identified. The results show that similar studies<br />should be done in other animals to better understand the nature of the DRB3 attributes.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goatsجداسازی و شناسایی مایکوپلاسما آگالاکتیه از اسپرم بز15916411161010.22092/ari.2017.111610ENS. A. PourbakhshMycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranA. R. AbtinMycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranA. AshtariMasterB. KheirkhahAssistant ProfessorM. A. BayatzadehMasterSalimeh AhangranBachelorJournal Article20160209Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,<br />which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease in<br />goats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semen<br />samples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented for<br />M. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was applied<br />to detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bp<br />fragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed for<br />both the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positive<br />and typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. In<br />addition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the samples<br />were shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M.<br />agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended to<br />concern semen as one of the significant sources for this pathogen and the possibility for transmission to the<br />female goats through semen is highlighted. Moreover, presence of this microorganism in semen could be<br />involved in infertility of goat population.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis lociLPS- PCR تاپینگ ایزولههای ایرانی پاستورلامولتوسیدای گوسفندی، براساس ژنهای بیوسنتزکننده هسته خارجی (1L تا 8LPS (L16517111160710.22092/ari.2017.111607ENS. F. Mirhaghgoye JalaliPasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, IranA.R. JabbariM. Esmael Zadassistant professor razi instituteJournal Article20160814<em>Pasteurella multocida</em> isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of <em>P. multocida</em> in its host. LPS can be used for the identification and classification of strains with somatic typing systems.The aim of this study was to identify the LPS genotypes of the ovine <em>P. multocida</em> isolates obtained from pneumonia cases in Iran. The LPS genotype of the isolates was determined using eight specific primers for LPS outer core biosynthesis loci. The LPS genes were amplified by polymerase chain reaction (PCR), then they were sequenced and compared to the sequences registered in the GenBank. Of the 32 ovine <em>P. multocida </em>isolates tested, 21 (65.62%) isolates belonged to genotype L6, 9 (28.12%) isolates contained genotype L3, 1 (3.12%) isolate had both L3 and L6 loci, and 1 (3.12%) isolate remained untypeable. The LPS-PCR was able to type 31 of 32 field ovine isolates from Iran. According to the phylogenetic analysis, L3 genotype isolates were grouped into two distinct lineages. LPS gene sequences among L6 genotypes of ovine <em>P. multocida</em> isolates from Iran and the related sequences in the GenBank were highly similar (>99.5%). LPS-PCR is an accurate genotyping method that was able to classify <em>P. multocida</em> strains into one of the eight distinct LPS genotypes.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strainتعیین ویژگیهای یک سلول-لاین گوسفند با منشاء لنفوئیدی حساس به سویه واکسینال ویروس عامل بیماری بورس عفونی طیور17317911160110.22092/ari.2017.111601ENS. ZandiehDepartment of Genetics, Islamic Azad University, Ahar, Iran.Mohsen LotfiDepartment of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran0000-0001-9902-7999M. KamalzadehHead of viral vaccine QC laboratoryN. ShiriQuality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.E. ParmourQuality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.A. EshaghiQuality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.S. MasoudiM. H. HablolvaridDepartment of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, IranA. ShoushtariManagement of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, IranH. GoudarziResearch and diagnosis of poultry diseases management of Razi Vaccine and Serum Research Institute, Karaj, Iran.S. M. J. Taher MofradDepartment of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, IranS. AmanpourImam Khomeini Hospital Cancer Institute, Tehran, IranJournal Article20150808Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin18118711160910.22092/ari.2017.111609ENN. Razmaraii0000-0002-6376-8290H. BabaeiDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, IranSchool of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, IranA. Mohajjel NayebiSchool of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, IranS. Ahdi KhosroshahiBiotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, IranS. FarajniaDrug Applied Research Center, Tabriz University of Medical SciencesJournal Article20160818Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iranتشخیص مولکولی و میکروسکوپی تیلریا اورینتالیس در گاوهای استان آذربایجان غربی، ایران18919511160510.22092/ari.2017.111605ENB. NarimaniDepartment of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IranN. Hoghooghi-RadDepartment of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, IranP. ShayanDepartment of Pathobiology, Faculty of Veterinary Science, Tehran University, Tehran, IranS. RahbariDepartment of Microbiology, Faculty of Specialized Veterinary Science, Branch of Science and Research, Islamic Azad University, Tehran, IranJournal Article20160601Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus <em>Theileria</em>. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect <em>T.annulata</em> DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for <em>Theileria</em> spp. and <em>T.orientalis </em>amplification, respectively<em>.</em> The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both <em>T.annulata</em> and <em>T.orientalis</em>. Out of 291 buffaloes, four samples (1.4%) were infected by <em>Theileria </em>genotypes, and two buffaloes (0.7%) were infected only by <em>T.orientalis.</em> The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that <em>T.orientalis</em> may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with <em>T.orientalis</em> for the first time.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran19720110838910.22034/ari.2017.108389ENA. DalimiDepartment of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran0000-0001-5591-9913F. JameieFaculty of Medical Sciences, Tarbiat Modares University0000-0003-3033-993XA. MohammadihaFaculty of Medical Sciences, Tarbiat Modares UniversityM. BaratiInfectious diseases Research Center, Aja University of Medical SciencesS. MolaeiFaculty of Medical Sciences, Tarbiat Modares UniversityJournal Article20160426<em>Hepatozoon </em>species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect <em>Hepatozoon canis</em> in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of 104 blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, 18S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of 897 bp for all the positive samples. Twenty-four out of the 104 (23.07%) samples were found to be positive for <em>H. canis</em>. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of 99% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of <em>H. canis</em> from Iran.Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343972320170901The Fauna and Active Season of Mosquitoes in West of Fars Province, Southwest of Iran20320811160310.22092/ari.2017.111603ENZ. SoltaniCommunicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, IranD. KeshavarziDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IranM. EbrahimiCommunicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, IranA. SoltaniResearch Centre for Health
Sciences, Department of Medical
Entomology and Vector Control,
School of Health, Shiraz
University of Medical Sciences,
Shiraz, IranM. J. Moemenbellah-FardDepartment of Medical Entomology and Vector Control, Research Centre for Health Sciences, School of Health, Shiraz University of Medical
Sciences, Shiraz, Iran.F. SoltaniCommunicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, IranH. Faramarzi4Department of Community Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.K. AmraeeDepartment of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranA. Elyasigomari5Department of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Journal Article20160704Culicidae are highly important for public health as they can be vectors of diseases and are responsible for a wide spectrum of infections. Five collection sites were selected randomly with regards to existing facilities in Firouzabad County. For collecting larvae and total catch for adult mosquitoes, sampling was carried out by dipping technique for collecting larvae and total catch for adult mosquitoes. A total of 689 adults and 1313 larvae of Culicidae were collected, of which 3 genera and 6 species of Culicidae were recognized, namely, <em>Anopheles superpictus</em>, <em>Anopheles d’thali</em>, <em>Culex sinaiticus</em>, <em>Culex theileri</em>, <em>Culex mimeticus,</em> and <em>Culiseta longiareolata. Cx. theileri</em> was the most frequent Culicidae collected at Firouzabad, with a total of 613 and 247 larval and adult specimens, respectively<em>.</em> The highest number of mosquitoes was collected in June (31.1%) and the lowest in May (3.4%). The mean temperatures in June and May were 31.3˚C and 28.2˚C, respectively. We found some vectors that are of medical and veterinary importance; our results could be applied in vector control programs that aim at eradication or control of mosquitoes in this area.