2024-03-29T18:25:26Z
https://archrazi.areeo.ac.ir/?_action=export&rf=summon&issue=10583
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
Investigation of Mycoplasma agalactiae in sheep in Kurdistan province by PCR
M.
Khezri
S.A.
Pourbakhsh
A.
Ashtari
B.
Rokhzad
Contagious agalactia (CA) is a serious disease syndrome of sheep and goats that is characterized by mastitis, arthritis, keratoconjunctivitis and, occasionally, abortion. Mycoplasma (M.) agalactiae is the main cause of the disease in sheep and goats. The aim of the present study was to detect M. agalactiae in conjunctival, synovial fluid, nasal, ear and milk samples in sheep herds with or without CA sign in Kurdistan province. One hundred and seventy three samples analyzed were taken from sheep herds reared in a CA endemic area. Of the samples tested, 130 were positive by PCR for Mycoplasma spp. and of these, 19 showed a positive result for M. agalactiae. M. agalactiae was detected in conjunctival (7/59), synovial fluid (1/8) and milk (11/21) samples. Ear and nasal swap samples were free of M. agalactiae. The results of 60 samples in sheep without signs of CA syndrome showed that Mycoplasma spp. and M. agalactiae were detected in 25 and 6 samples, respectively. Our findings indicate that in Kurdistan province; M. agalactiae was not the main etiological agents of the CA syndrome. Also, this species can be isolated from animals without clinical signs of disease. Our results suggested that milk secretion is suitable for PCR detection of M. agalactiae.
Contagious agalactia
Mycoplasma agalactiae
PCR
Sheep
Kurdistan province
2015
07
01
73
80
https://archrazi.areeo.ac.ir/article_103965_bb4c64a5b171be3a7b2f98fb84e0d9bd.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
Detection of Salmonella spp. from some wild captive herbivores in Iran and determination of serogroup, antibiotic susceptibility and presence of invA gene in the isolated strains
A.
Koochakzadeh
T.
Zahraei Saleh
B.
Nayeri Fasaei
M.
Askari Badouei
K.
Oskouizadeh
Salmonella spp. are zoonotic enteric bacteria able to infect humans, livestock and wildlife. The aim of this study was to investigate the prevalence of Salmonella (spp.) and to determine antibiotic susceptibility, serogrouping and presence of invA gene (Salmonella Invasion Gene A) in the detected strains in wild captive herbivores in Iran. The fecal samples of 103 animals from 8 different species were evaluated for presence of the Salmonella. Results indicated that 9.7% of animals were positive for Salmonella, all of these strains belonged to D serogroup, also all the Salmonella strains harbored invA gene. In vitro antibiotic activities of 10 antibiotic substances against the isolates were determined by disc diffusion test. The highest rate of resistance was against Amoxicillin (100%), Tetracycline (80%), Neomycin (60%), Lincospectin (50%) and Enrofloxacin (40%), Resistance to Furazolidone wasn’t observed. In conclusion, these species can act as a reservoir for Salmonella. Also, since the study was conducted in some parts of Iran, a more accurate conclusion needs more distributed sampling. To our knowledge this is the first study which reports the fecal shedding of Salmonella from Cervus elaphus, Capra aegarus, Oryx leucoryx, Ammotragus lervia and Lama glama in Iran.
Salmonella
wild captive herbivores
antibiogram
serogroup
invA gene
Iran
2015
07
01
81
87
https://archrazi.areeo.ac.ir/article_103966_fe98d59d61c5e7cd94c9eda51b005c0c.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
The species composition of mosquitoes (Diptera: Culicidae) in the Mahshahr district, Khuzestan province, southwest of Iran
R.
Farhadinejad
M.
Mousavi
K.
Amraee
There is very little information about the mosquito fauna of Mahshahr district. The present study gives an overview of data on the species composition and distribution of mosquitoes in the Mahshahr district, Khuzestan province, southwest of Iran. In order to study the mosquito fauna, the samples were collected from May to November 2012 using dipping and total catch methods from seven villages (Soveireh, Hadid, Seraimeh, Meksar, Hashtcheh, Seyed Yahya and Maghtoo Sofla) and two towns (Shahrake Meghdad and Shahrake Dastgheib) in the Mahshahr district. Totally 1023 adult mosquitoes and 1618 larvae were collected and four genera along with eleven species of Culicidae were identified, including; Anopheles stephensi Liston, An. superpictus Grassi, An. dthali Patton, An. pulcherrimus Theobald, Culex pusillus Macquart, Cx. quinquefasciatus Say, Cx. sitaiticus Kirkpatrick, Cx. theileri Theobald, Cx. perexiguus Theobald, Ochlerotatus caspius Pallas and Culiseta longiareolata Macquart. There are some potential or proven vectors of different human and domesticated animal pathogens in Mahshahr district. The ecology of these species of Mahshahr district need to be investigated extensively.
species composition
Mosquito
Anopheles
Culex
Culiseta
Ochlerotatus
Mahshahr
2015
07
01
89
95
https://archrazi.areeo.ac.ir/article_103967_bad984b2212d50a9fe06f61aaac37a27.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
Plasma pharmacokinetics of pioglitazone following oral or intravenous administration in Holstein cows
A.R.
Yousefi
H.
Kohram
A.
Zare Shahneh
M.J.
Zamiri
F.
Ghaziani
M.
Kazemi Khoozani
S.M.
Ghoreishi
H.A.
Arab
Pioglitazone belongs to the thiazolidinedione (TZD) class of antidiabetic agents, with proven efficacy in increasing insulin sensitivity and in the treatment of type 2 diabetes mellitus in humans. Pioglitazone has been proposed as a potential feed additive to reduce insulin resistance and consequently some of the metabolic disorders in transition cows. This study was aimed at determining the pharmacokinetic parameters of pioglitazone following oral administration (PO) or intravenous (IV) injection. Six lactating Holstein cows were randomly assigned into two groups (n=3 cows per group) in a crossover design, and administered with pioglitazone (8 mg/kg BW) either per-oral (PO) or intravenously (IV), with an 8-day washout period. Blood samples were collected from the jugular vein before and up to 48 h after pioglitazone administration. Plasma pioglitazone concentration was determined by HPLC. The data were analyzed using a non-compartmental model for PO route, and a two-compartmental model for the IV route. The bioavailability of PO-administered pioglitazone was 58% and the highest plasma concentration (Cmax), the time (tmax) at which the drug reached Cmax, half-life (t1/2), absorption rate constant (kab) and elimination rate constant (kel) were 11.57±1.44 μg/mL, 5.67±0.07 h, 7.10±0.32 h, 0.28±0.09 h-1 and 0.10±0.013 h-1, respectively. Elimination half-life (t1/2β), volume distribution (Vss) and elimination rate constant (kel) after IV injection were 5.10±0.62 h, 0.12±0.01 L/kg and 0.47±0.06 h-1, respectively. Because of the relatively high bioavailability and half-life, pioglitazone may be useful for oral administration as an insulin-sensitizing agent in dairy cows.
Bioavailability
Pharmacokinetics
Pioglitazone insulin resistance
Dairy cow
2015
07
01
97
104
https://archrazi.areeo.ac.ir/article_103968_627a322746d0120352546a6c705fd03a.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
Effect of substitute milk on growth and breeding rates of hysterectomy derived guinea pigs neonates
R.
Fallahi
In this study, considering the normal ingredients in guinea pig's milk, by using cow milk, mixture of vitamins and minerals, a milk substitute was prepared and sterilized by autoclaving and filtration methods. In food analysis of produced milk, the important substances values, such as protein, fat and ash, were suitable. Milk substitute was fed to four groups of newborn's guinea pigs that were obtained by hysterectomy from out bred color Pirbright conventional parents, and maintained in the sterile environment inside laminar flow. The health, growth and reproductive parameters were evaluated during 6 months in four treated groups at the suckling age (milk substitute) and solid pellets feeding stage and these values were compared with four coequal's control groups. The results show, all young fed with substitute milk, reached to puberty at normal age (3-4 months old ), The males, had the normal fecundity and the duration of pregnancy in all females were in the normal range (22-25 weeks), litter size (4-5) and birth weight (70-100g) were in the normal ranges. In this study the preparation of suitable substitute milk and also a part of the method and protocol for production of the specific pathogen free (SPF) animals were introduced and put into the practice.
Substitute milk
Growth
Breeding
Hysterectomy
Guinea pig
2015
07
01
105
110
https://archrazi.areeo.ac.ir/article_103969_74944f0713ea9794a04a3db94b022d43.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
A Survey of Scientific Outputs of Iranian Researchers in the Field of Poisonous Animals in Web of Science: a Scientometric Study
A.
Yousefi
A.
Zare Mirakabadi
ISI Web of Science (WOS) is the world's most prestigious database that is used in order to evaluate and rank the countries, researchers, institutions, and universities. Therefore, determining the status of Iranian articles in this database in the field of poisonous and venomous animals is important. Quantitative and qualitative assessments of scientific outputs are the main goals of this study. In order to conduct this research, 220 articles written by Iranian researchers during 1973 to 2010 in the field of poisonous animals which have been indexed in Web of Science were reviewed. Scientometric, survey and citation analysis methods have been used. In this research, number of papers in different years, annual growth rate of papers, international collaboration of Iranian researchers with their counterparts from other countries, impact factor, average citation per paper in comparison with some selected countries, subject categories, and the most prolific institutes and authors have been surveyed. Results of the present study showed that from 2004 onwards, the number of papers increasingly enhanced. The most collaboration of Iranian authors has been done respectively with their counterparts and colleagues from USA, Belgium and Scotland; Poisonous animals articles have had the most interaction with the fields of toxicology and pharmacology. The mean impact factor was 1.731 and the average citation per paper was 4.15. Tehran University, Razi Vaccine and Serum Research Institute, Tehran University of Medical Sciences and Tarbiat Modarres University were the most Iranian prolific universities and institutes respectively. Number of articles in the field of poisonous animals has been increased significantly in recent years. The most collaboration was with USA and European countries respectively. The average citation per paper of Iranian articles in poisonous animals is lower than selected countries. Besides universities, Razi Vaccine and Serum Research Institute, is among the most prolific universities and institutes. The mean impact factor of Iran in poisonous animals has relatively good position in comparison with other fields of Iran, but lower than other fields in some mentioned countries.
Poisonous animals
Articles
Iran
ranking
Scientometrics
ISI Web of Science Database
2015
07
01
111
117
https://archrazi.areeo.ac.ir/article_103970_cb1a63a216d4f3a6472c913cddd96a39.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
Absolute quantification of murine interleukine-4, interleukine- 10 and interferon-γ gene transcripts using Real Time PCR
M.
Esmaelizad
S.
Jafari
Alireza
Rafiei
The study of cytokines gene expression is quite important in various conditions of health and disease for the evaluation of clinical responses to new vaccination approaches. An absolute quantification is based on a calibration curve and production of standard controls to achieve more reliable results than in relative system. In this study we attempted to construct standard controls to evaluate the murine immune response. The number of 10 Balb/c mice immunized with hydatid cyst fluid subcutaneously with two week intervals to induce transcription of Th1 and Th2 related cytokines. Three Pairs of primers were designed to amplification of interleukine-4, interleukine-10 and interferon-γ by Oligo software. Partial sequences of three cytokine genes were cloned into pTZ57T vector. Three recombinant plasmids were purified and serial dilutions were prepared. Real time qPCR carried out using SYBR-Green I fluorescence dye and standard curves were provided by the 7500 ABI SDS software based on the exact concentration of dilutions and the amplification plots. Results showed that this method was able to evaluate the cytokines mRNA levels less than 0.01pg (~150 copy). We concluded that absolute real-time qPCR can be successfully applied to the quantification of antigen-induced cytokines.
real time PCR
Cytokine
IL4
IL10
IFN
γ
2015
07
01
119
125
https://archrazi.areeo.ac.ir/article_103971_f4527141b0568e688a38bc55c49084ef.pdf
Archives of Razi Institute
ARIJ
0365-3439
0365-3439
2015
70
2
A serological survey on Leptospiral infection in companion rabbits referred to Veterinary Hospital of Shahid Chamran University of Ahvaz
B.
Mosallanejad
M.
Ghorbanpour Najafabad
R.
Avizeh
G.
Abdollahpour
Leptospirosis is one of the major zoonotic diseases worldwide. This disease is caused by various serovars of Leptospira interrogans sensu lato infection. Although the rabbits are known to be one of the reservoirs and transmission sources of leptospirosis, but the status of this infection in rabbits in Iran remains unknown, so this survey was conducted to evaluate the seroprevalence of leptospiral infection in companion rabbits in Ahvaz district from September 2010 to December 2013. Blood samples were taken from 68 companion rabbits, referred to veterinary hospital of shahid Chamran University of Ahvaz. Sera were screened for antibodies against serovars of Canicola, Icterohaemorrhagiae, Grippotyphosa, Ballum, Hardjo, Pomona, Australis and Tarassovi using the microscopic agglutination test (MAT). From a total of 68 rabbits, 19 (27.94%) were serologically positive for the serovars of L. Icterohaemorrhagiae, Tarassovi, Grippotyphosa, Hardjo, Pomona and Australis. The positive titers were detected between 1:100 to 1:200 dilutions. The predominant titers were directed against serovars of Icterohaemorrhagiae (36.84%; 7 out of 19) and Tarassovi (36.84%; 7 out of 19) and followed by Hardjo (21.05%; 4 out of 19), Australis (10.53%; 2 out of 19), Pomona (5.26%; 1 out of 19) and rippotyphosa (5.26%; 1 out of 19). Antibodies against more than one serovar (mixed infections) were detected in three samples (as Pomona+Hardjo and Icterohaemorrhagiae+ Tarassovi). The prevalence was significantly higher in adult rabbits above 3 years (37.04%; 10 out of 27) and between 1-3 years (36.36%; 8 out of 22) compared with rabbits less than 1 years (5.26%; 1 out of 19) (P0.05). This survey indicated that serovars of L. Icterohaemorrhagiae and Tarassovi were predominant in the rabbits of this area and can be a source of infection for humans. The obtained results provide useful information on the epidemiology of leptospirosis in this species, which has not been previously investigated.
Leptospirosis
Seroprevalence
Rabbit
zoonosis
Ahvaz
Iran
2015
07
01
127
133
https://archrazi.areeo.ac.ir/article_103972_a7703720a0fdff72327fac233cd78105.pdf