TY - JOUR ID - 109836 TI - A reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2 JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Tajbakhsh, Amir AU - Rezatofighi, Elham AU - Mirzadeh, Khalil AU - Pourmahdi, Mahdi AD - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran AD - Department of Animal Science and Food, Khouzastan Ramin Agriculture and Natural Resources University, Ahvaz, Iran AD - Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Y1 - 2017 PY - 2017 VL - 72 IS - 2 SP - 73 EP - 81 KW - Bovine viral diarrhea KW - Reverse Transcriptase-Loop mediated isothermal amplification assay KW - Reverse Transcriptase-Polymerase Chain Reaction DO - 10.22092/ari.2017.109836 N2 - Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct regions on the target RNA based on a highly conserved sequence in the 5΄ UTR of the BVDV genome. Eighty blood specimens were collected from bovines suspected to suffer from BVDV infection, and were tested in parallel by RT-LAMP and RT-PCR. Twenty four of these samples were positive by RT-LAMP, while twenty were positive by RT-PCR. The RT-LAMP detection limit was estimated to be approximately 70PFU /mL of virus. Comparison of RT-PCR with RT-LAMP in this study revealed the recent developed RT-LAMP a highly sensitive and specific for BVD virus detection in the clinical samples. UR - https://archrazi.areeo.ac.ir/article_109836.html L1 - https://archrazi.areeo.ac.ir/article_109836_1f4214fcfc32304b8e5b02f7d8dec67d.pdf ER -