TY - JOUR ID - 103956 TI - Identification of Avian Salmonella Isolates by PCR-RFLP Analysis of a fliC Gene Fragment JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Moradi Bidhendi, S. AU - Alaei, F. AU - Khaki, P. AU - Ghaderi, R. AD - Y1 - 2015 PY - 2015 VL - 70 IS - 1 SP - 1 EP - 6 KW - Salmonella KW - Avian KW - fliC gene KW - PCR KW - RFLP KW - HhaI restriction endonuclease DO - 10.7508/ari.2015.01.001 N2 - The genus of Salmonella is very polymorphic and comprised of a number of genetically closely related serotypes. It is one of the emerging pathogen in food-borne disease which is often found in contaminated chicken eggs. Salmonella enterica is considered one of the major pathogens in public health worldwide. A total of 31 Salmonella isolates identified by specific antisera, which included Salmonella enteritidis (51.6%), Salmonella typhimurium (25.8%), Salmonella infantis (19.4%) and Salmonella colindale (3.2%). DNA was extracted using phenol- chloroform- isoamylalchol method. All the isolates showed fliC gene (1500bp) by using specific primers. PCR products were subjected to digestion using HhaI restriction endonuclease. PCR- RFLP results showed 3 patterns between all isolates. Our research gained in this study demonstrated that using HhaI restriction endonuclease could differentiate Salmonella   enteritidis and Salmonella colindale but there is similarity between pattern of Salmonella typhimurium and Salmonella   infantis. UR - https://archrazi.areeo.ac.ir/article_103956.html L1 - https://archrazi.areeo.ac.ir/article_103956_7c50e676da64ee29cdfbae30d14d6dad.pdf ER -