ORIGINAL_ARTICLE
Analysis of variations, structures, and phylogenic characteristics of bovine leukocyte antigen DRB3 exon2
Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular andevolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustrate some novelpractical evidence on interspecies diversity, the resistance /susceptibility points in cattle breeding, and vaccinedesign. Initially, BoLA-DRB3.2* alleles and orthologous exons in the selected livestock were retrieved andchecked. In the next step, the secondary/tertiary structure of BoLA-DRB3.2*24 gene product was modeled andvalidated. Then, hypervariable regions (HVRs) of alleles were identified by hybrid approaches. In the last step,interspecies relationship, allele’s phylogeny/grouping, and estimate of average evolutionary divergence wereexplored. Shannon entropy variation analysis showed eight HVRs and three semi-variable regions in BoLADRB3.2*alleles. These HVRs were present in all the three sub-structures and dominantly existed in alpha helix.In addition, strong relationships and little diversity were noted in phylogenetic trees of cattle, buffaloes, sheep,and goats. Furthermore, there was some evidence on divergence of DRB3 before speciation among thementioned species and possibility of cross prediction resistance/susceptibility alleles. Finally, DRB3 alleles weregrouped into seven clusters, and older and newer alleles were identified. The results show that similar studiesshould be done in other animals to better understand the nature of the DRB3 attributes.
https://archrazi.areeo.ac.ir/article_111611_50130e38448fdbd64a0f96493963014f.pdf
2017-09-01
147
157
10.22092/ari.2017.111611
BoLA-DRB3.2
Cattle
Variation
modeling
phylogeny
M. M.
Ranjbar
mm.ranjbar.phd@gmail.com
1
Department of Viral Animal Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
AUTHOR
S.
Ataei
ataei111@hotmail.com
2
Department of Avian Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
LEAD_AUTHOR
Gh.
Nikbakht Brujeni
nikbakht@ut.ac.ir
3
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
S.
Golabdar
golabdar@ut.ac.ir
4
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Behl, J.D., Verma, N.K., Tyagi, N., Mishra, P., Behl, R., Joshi, B.K., 2012. The Major Histocompatibility Complex in Bovines: A Review. ISRN Veterinary Science 2012, 12.
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Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., et al., 2000. The Protein Data Bank. Nucleic Acids Res 28, 235-242.
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Burt, D.W., 2009. The cattle genome reveals its secrets. J Biol 8, 36.
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De, S., Singh, R.K., Butchaiah, G., 2002. MHC-DRB exon 2 allele polymorphism in Indian river buffalo (Bubalus bubalis). Anim Genet 33, 215-219.
4
Gowane, G.R., Sharma, A.K., Sankar, M., Thirumurugan, P., Narayanan, K., Subramaniam, S., et al., 2013. Evaluation of Genetic and Environmental Parameters Determining Antibody Response Induced by Vaccination Against Foot and Mouth Disease. Agricultural Research 2, 140-147.
5
Gupta, S.K., Srivastava, M., Akhoon, B.A., Gupta, S.K., Grabe, N., 2012. In silico accelerated identification of structurally conserved CD8+ and CD4+ T-cell epitopes in high-risk HPV types. Infect Genet Evol 12, 1513-1518.
6
Gupta, S.K., Srivastava, M., Akhoon, B.A., Smita, S., Schmitz, U., Wolkenhauer, O., et al., 2011. Identification of immunogenic consensus T-cell epitopes in globally distributed influenza-A H1N1 neuraminidase. Infect Genet Evol 11, 308-319.
7
Jeong, H.J., Bhuiyan, M.S.A., Lee, J.S., Yu, S.L., Sang, B.C., Yoon, D., et al., 2007. Characterization of BoLA-DRB3.2 Alleles in Hanwoo (Korean cattle) by Sequence Based Typing (SBT). Asian-Australas J Anim Sci 20, 1791-1797.
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Johansson, F., Toh, H., 2010. A comparative study of conservation and variation scores. BMC Bioinformatics 11, 388.
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MacCallum, R.M., Martin, A.C., Thornton, J.M., 1996. Antibody-antigen interactions: contact analysis and binding site topography. J Mol Biol 262, 732-745.
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Marti-Renom, M.A., Stuart, A.C., Fiser, A., Sanchez, R., Melo, F., Sali, A., 2000. Comparative protein structure modeling of genes and genomes. Annu Rev Biophys Biomol Struct 29, 291-325.
11
Miyasaka, T., Takeshima, S.N., Matsumoto, Y., Kobayashi, N., Matsuhashi, T., Miyazaki, Y., et al., 2011. The diversity of bovine MHC class II DRB3 and DQA1 alleles in different herds of Japanese Black and Holstein cattle in Japan. Gene 472, 42-49.
12
Nielsen, M., Justesen, S., Lund, O., Lundegaard, C., Buus, S., 2010. NetMHCIIpan-2.0 - Improved pan-specific HLA-DR predictions using a novel concurrent alignment and weight optimization training procedure. Immunome Res 6, 9.
13
Nikbakht, G., Ranjbar, M.M., Ghasemi, F., Asadian, F., 2012. Allelic polymorphism in exon 2 of the BoLA-DRB3 gene in Iranian Holstein cows Method and Material. Anim Prod Res 1, 33- 41.
14
Paital, B., Kumar, S., Farmer, R., Tripathy, N.K., Chainy, G.B., 2011. In silico prediction and characterization of 3D structure and binding properties of catalase from the commercially important crab, Scylla serrata. Interdiscip Sci 3, 110-120.
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16
Ranjbar, M.M., Gupta, S.K., Ghorban, K., Nabian, S., Sazmand, A., Taheri, M., et al., 2015. Designing and modeling of complex DNA vaccine based on tropomyosin protein of Boophilus genus tick. Appl Biochem Biotechnol 175, 323-339.
17
Ranjbar, M.M., Nikbakht, G., Ghadrdan Mashhadi, A.R., Dabbaghyan, M., 2016. Study of BuLA-DRB3 polymorphism in Khuzestan river buffaloes. J Vet Res 71, 33-40.
18
Rupp, R., Hernandez, A., Mallard, B.A., 2007. Association of bovine leukocyte antigen (BoLA) DRB3.2 with immune response, mastitis, and production and type traits in Canadian Holsteins. J Dairy Sci 90, 1029-1038.
19
Russell, G.C., Fraser, D.C., Craigmile, S., Oliver, R.A., Dutia, B.M., Glass, E.J., 2000. Sequence and transfection of BoLA-DRB3 cDNAs. Anim Genet 31, 219-222.
20
Sena, L., Schneider, M.P., Brenig, B., Honeycutt, R.L., Womack, J.E., Skow, L.C., 2003. Polymorphisms in MHC-DRA and -DRB alleles of water buffalo (Bubalus bubalis) reveal different features from cattle DR alleles. Anim Genet 34, 1-10.
21
Sharif, S., Mallard, B.A., Sargeant, J.M., 2000. Presence of glutamine at position 74 of pocket 4 in the BoLA-DR antigen binding groove is associated with occurrence of clinical mastitis caused by Staphylococcus species. Veterinary Immunology and Immunopathology 76, 231-238.
22
Sharif, S., Mallard, B.A., Wilkie, B.N., Sargeant, J.M., Scott, H.M., Dekkers, J.C., et al., 1999. Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) with production traits in Canadian dairy cattle. Anim Genet 30, 157-160.
23
Takeshima, S.-n., Ikegami, M., Morita, M., Nakai, Y., Aida, Y., 2001. Identification of new cattle BoLA-DRB3 alleles by sequence-based typing. Immunogenetics 53, 74-81.
24
Takeshima, S., Saitou, N., Morita, M., Inoko, H., Aida, Y., 2003. The diversity of bovine MHC class II DRB3 genes in Japanese Black, Japanese Shorthorn, Jersey and Holstein cattle in Japan. Gene 316, 111-118.
25
Takeshima, S.N., Matsumoto, Y., Aida, Y., 2009. Short communication: Establishment of a new polymerase chain reaction-sequence-based typing method for genotyping cattle major histocompatibility complex class II DRB3. J Dairy Sci 92, 2965-2970.
26
Tizard, I.R., 2013. Veterinary Immunology - E-Book, Elsevier Health Sciences.
27
Tomar, N., De, R.K., 2010. Immunoinformatics: an integrated scenario. Immunology 131, 153-168.
28
Tramontano, A., Leplae, R., Morea, V., 2001. Analysis and assessment of comparative modeling predictions in CASP4. Proteins Suppl 5, 22-38.
29
Wang, K., Sun, D., Zhang, Y., 2008. Sequencing of 15 new BoLA-DRB3 alleles. Int J Immunogenet 35, 331-332.
30
Yoshida, T., Mukoyama, H., Furuta, H., Kondo, Y., Takeshima, S.-n., Aida, Y., et al., 2009a. Association of the amino acid motifs of BoLA-DRB3 alleles with mastitis pathogens in Japanese Holstein cows. Animal Science Journal 80, 510-519.
31
Yoshida, T., Mukoyama, H., Furuta, H., Kondo, Y., Takeshima, S.N., Aida, Y., et al., 2009b. Association of BoLA-DRB3 alleles identified by a sequence-based typing method with mastitis pathogens in Japanese Holstein cows. Ani m Sci J 80, 498-509.
32
ORIGINAL_ARTICLE
Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats
Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease ingoats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semensamples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented forM. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was appliedto detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bpfragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed forboth the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positiveand typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. Inaddition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the sampleswere shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M.agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended toconcern semen as one of the significant sources for this pathogen and the possibility for transmission to thefemale goats through semen is highlighted. Moreover, presence of this microorganism in semen could beinvolved in infertility of goat population.
https://archrazi.areeo.ac.ir/article_111610_714d06eb229f24c70e07977dcce074e6.pdf
2017-09-01
159
164
10.22092/ari.2017.111610
Goat buck
Lipoprotein gene
Mycoplasma agalactiae
Semen
16srRNA
S. A.
Pourbakhsh
poursaba@yahoo.com
1
Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
LEAD_AUTHOR
A. R.
Abtin
dvm_alirezaabtin@yahoo.com
2
Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
AUTHOR
A.
Ashtari
abbass.ashtari@gmail.com
3
Master
AUTHOR
B.
Kheirkhah
babakkheirkhah@yahoo.com
4
Assistant Professor
AUTHOR
M. A.
Bayatzadeh
ma.bayatzadeh@yahoo.com
5
Master
AUTHOR
Salimeh
Ahangran
salroze@yahoo.com
6
Bachelor
AUTHOR
Abo-Elnaga, T.R., Gouda, A.S.A., Kamel, Y.M., Mahmoud, M.A., Osman, W.A., 2012. Application of the Polymerase Chain Reaction for identification of Mycoplasma isolated from aborted she camel. Glob Vet 8, 386-392.
1
Ak, K., Ak, S., Gurel, M., Hasoksuz, A., Barau, Y., Ozturkeler, I., et al., 1995. Experimental studies on the effects of Mycoplasma agalactiae on the spermatozoa and genital organs of rams. Pendik Vet Mikrobiyol Derg 26, 139-155.
2
Bergonier, D., Berthelot, X., Pourmarat, F., 1997. Contagious agalactia of small ruminants current of knowledge concerning epidemiology diagnosis and control. Rev Sci Techenol 16, 848-873.
3
Bermudez, V.M., Miller, R.B., Rosendal, S., Fernando, M.A., Johnson, W.H., O'Brien, P.J., 1992. Measurement of the cytotoxic effects of different strains of Mycoplasma equigenitalium on the equine uterine tube using a calmodulin assay. Can J Vet Res 56, 331-338.
4
Bielanski, A., Devenish, J., Phipps-Todd, B., 2000. Effect of Mycoplasma bovis Mycoplasma bovigenitalium in semen on fertilization and association with in vitro produced morula and blastocyst stage embryos. Theriogenology 53, 1213-1223.
5
Breard, A., Poumarat, F., 1988. [Isolation of Mycoplasma capricolum from bull semen]. Rev Elev Med Vet Pays Trop 41, 149-150.
6
Byrne, J., Brennan, P., McCormack, R., Ball, H.J., 1999. Isolation of <em>Mycoplasma bovis</em> from the abomasal contents of an aborted bovine fetus. Vet Record 144, 211.
7
Corrales, J.C., Esnal, A., De la Fe, C., Sánchez, A., Assunçao, P., Poveda, J.B., et al., 2007. Contagious agalactia in small ruminants. Small Rumin Res 68, 154-166.
8
de la Fe, C., Amores, J., Martin, A.G., Sanchez, A., Contreras, A., Corrales, J.C., 2009. Mycoplasma agalactiae detected in the semen of goat bucks. Theriogenology 72, 1278-1281.
9
de la Fe, C., Gomez Martin, A., Amores, J., Corrales, J.C., Sanchez, A., Poveda, J.B., et al., 2010. Latent infection of male goats with Mycoplasma agalactiae and Mycoplasma mycoides subspecies capri at an artificial insemination centre. Vet J 186, 113-115.
10
Dedieu, L., Mady, V., Lefevre, P.-C., 1995. Development of two PCR assays for the identification of mycoplasmas causing contagious agalactia. FEMS Microbiology Letters 129, 243-249.
11
Gil, M.C., Peña, F.J., Hermoso De Mendoza, J., Gomez, L., 2003. Genital Lesions in an Outbreak of Caprine Contagious Agalactia Caused by Mycoplasma agalactiae and Mycoplasma putrefaciens. J Vet Med 50, 484-487.
12
Givens, M.D., Marley, M.S., 2008. Pathogens that cause infertility of bulls or transmission via semen. Theriogenology 70, 504-507.
13
Gomez-Martin, A., Corrales, J.C., Amores, J., Sanchez, A., Contreras, A., Paterna, A., et al., 2012. Controlling contagious agalactia in artificial insemination centers for goats and detection of Mycoplasma mycoides subspecies capri in semen. Theriogenology 77, 1252-1256.
14
Hasso, S.A., Al-Aubaidi, J.M., Al-Darraji, A.M., 1993. Contagious agalactia in goats: It's severity as related to the route of infection and pregnancy. Small Rumin Res 10, 263-275.
15
Jones, G.E., Rae, A.G., Holmes, R.G., Lister, S.A., Jones, J.M., Grater, G.S., et al., 1983. Isolation of exotic mycoplasmas from sheep in England. Vet Rec 113, 540.
16
Jurmanova, K., Sterbova, J., 1977. Correlation between impaired spermatozoan motility and mycoplasma findings in bull semen. Vet Rec 100, 157-158.
17
Kissi, B., Juhasz, S., Stipkovits, L., 1985. Effect of mycoplasma contamination of bull semen on fertilization. Acta Vet Hung 33, 107-117.
18
Kojima, A., Takahashi, T., Kijima, M., Ogikubo, Y., Nishimura, M., Nishimura, S., et al., 1997. Detection of Mycoplasma in avian live virus vaccines by polymerase chain reaction. Biologicals 25, 365-371.
19
Kotani, H., Nagatomo, H., Ogata, M., 1980. Isolation and Serological Comparison of Ureaplasmas from Goats and Sheep. Japanese J Vet Sci 42, 31-40.
20
Lambert, M., 1987. Contagious agalactia of sheep and goats. Rev Sci Technol OIE 6, 699-711.
21
Le Grand, D., Poumarat, F., Martel, J.L., 1995. [Genital Ureaplasma diversum infection: investigations in cattle in France]. Vet Res 26, 11-20.
22
Miller, R., Chelmonska-Soyta, A., Smits, B., Foster, R., Rosendal, S., 1994. Ureaplasma Diversum As a Cause of Reproductive Disease in Cattle. Vet Clin North Am: Food Anim Prac 10, 479-490.
23
Nicholas, R.A.J., Ayling, R.D., 2003. Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci 74, 105-112.
24
Nunez-Calonge, R., Caballero, P., Redondo, C., Baquero, F., Martinez-Ferrer, M., Meseguer, M.A., 1998. Ureaplasma urealyticum reduces motility and induces membrane alterations in human spermatozoa. Hum Reprod 13, 2756-2761.
25
OIE., 2008. Contagious agalactia
26
Singh, N., Rajya, B.S., Mohanty, G.C., 1974. Granular vulvovaginitis (GVV) in goats associated with Mycoplasma agalactiae. Cornell Vet 64, 435-442.
27
Spergser, J., Aurich, C., Aurich, J.E., Rosengarten, R., 2002. High prevalence of mycoplasmas in the genital tract of asymptomatic stallions in Austria. Veterinary Microbiology 87, 119-129.
28
Sylla, L., Stradaioli, G., Manuali, E., Rota, A., Zelli, R., Vincenti, L., et al., 2005. The effect of Mycoplasma mycoides ssp. mycoides LC of bovine origin on in vitro fertilizing ability of bull spermatozoa and embryo development. Anim Reprod Sci 85, 81-93.
29
Tola, S., Idini, G., Manunta, D., Galleri, G., Angioi, A., Rocchigiani, A.M., et al., 1996. Rapid and specific detection of Mycoplasma agalactiae by polymerase chain reaction. Vet Microbiol 51, 77-84
30
ORIGINAL_ARTICLE
LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis loci
Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic typing systems.The aim of this study was to identify the LPS genotypes of the ovine P. multocida isolates obtained from pneumonia cases in Iran. The LPS genotype of the isolates was determined using eight specific primers for LPS outer core biosynthesis loci. The LPS genes were amplified by polymerase chain reaction (PCR), then they were sequenced and compared to the sequences registered in the GenBank. Of the 32 ovine P. multocida isolates tested, 21 (65.62%) isolates belonged to genotype L6, 9 (28.12%) isolates contained genotype L3, 1 (3.12%) isolate had both L3 and L6 loci, and 1 (3.12%) isolate remained untypeable. The LPS-PCR was able to type 31 of 32 field ovine isolates from Iran. According to the phylogenetic analysis, L3 genotype isolates were grouped into two distinct lineages. LPS gene sequences among L6 genotypes of ovine P. multocida isolates from Iran and the related sequences in the GenBank were highly similar (>99.5%). LPS-PCR is an accurate genotyping method that was able to classify P. multocida strains into one of the eight distinct LPS genotypes.
https://archrazi.areeo.ac.ir/article_111607_ec494786bf8c99c264e682d2b81fb9f7.pdf
2017-09-01
165
171
10.22092/ari.2017.111607
P. multocida
Sheep
LPS outer core
PCR-typing
S. F.
Mirhaghgoye Jalali
jalali.fahimeh1950@gmail.com
1
Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
AUTHOR
A.R.
Jabbari
a.jabbari@rvsri.ac.ir
2
LEAD_AUTHOR
M.
Esmael Zad
m.esmaelizad@rsri.ac.ir
3
assistant professor razi institute
AUTHOR
Brogden, K.A., Rebers, P.A., 1978. Serologic examination of the Westphal-type lipopolysaccharides of Pasteurella multocida. Am J Vet Res 39, 1680-1682.
1
Christensen, J.P., Bisgaard, M., 2000. Fowel cholera. Rev Sci Tech OIE 19, 626-637.
2
Ewers, C., Lübke-Becker, A., Bethe, A., Kießling, S., Filter, M., Wieler, L.H., 2006. Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Veterinary Microbiology 114, 304-317.
3
Harper, M., Boyce, J.D., Adler, B., 2006. Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett 265, 1-10.
4
Harper, M., Cox, A.D., Adler, B., Boyce, J.D., 2011a. Pasteurella multocida lipopolysaccharide: the long and the short of it. Vet Microbiol 153, 109-115.
5
Harper, M., Michael, F., Vinogradov, E., John, M., Steen, J.A., Van Dorsten, L., et al., 2013a. Structure and biosynthetic locus of the lipopolysaccharide produced by pasteurella multocida serovars 8 and 13 and the identification of a novel phosphor-glycero moiety. Glycobiology 23, 286-294.
6
Harper, M., St Michael, F., John, M., Steen, J., van Dorsten, L., Parnas, H., et al., 2014. Structural analysis of lipopolysaccharide produced by Heddleston serovars 10, 11, 12 and 15 and the identification of a new Pasteurella multocida lipopolysaccharide outer core biosynthesis locus, L6. Glycobiology 24, 649-659.
7
Harper, M., St Michael, F., John, M., Vinogradov, E., Adler, B., Boyce, J.D., et al., 2011b. Pasteurella multocida Heddleston serovars 1 and 14 express different lipopolysaccharide structures but share the same lipopolysaccharide biosynthesis outer core locus. Vet Microbiol 150, 289-296.
8
Harper, M., St Michael, F., John, M., Vinogradov, E., Steen, J.A., van Dorsten, L., et al., 2013b. Pasteurella multocida Heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity. J Bacteriol 195, 4854-4864.
9
Harper, M., St Michael, F., Vinogradov, E., John, M., Boyce, J.D., Adler, B., et al., 2012. Characterization of the lipopolysaccharide from Pasteurella multocida Heddleston serovar 9: identification of a proposed bi-functional dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis enzyme. Glycobiology 22, 332-344.
10
Heddleston, K.L., Gallagher, J.E., Rebers, P.A., 1972. Fowl cholera: gel diffusion precipitin test for serotyping Pasteruella multocida from avian species. Avian Dis 16, 925-936.
11
Raetz, C.R.H., Whitfield, C., 2002. Lipopolysaccharide Endotoxins. Annual Review of Biochemistry 71, 635-700.
12
Shayegh, J., Dolgari-Sharaf, J., Mikaili, P., Namvar, H., 2009. Pheno- and genotyping of Pasteurella multocida isolated from goat in Iran. Af J Biotechnol 8, 3707-3710.
13
Singh, R., Blackall, P.J., Remington, B., Turni, C., 2013. Studies on the presence and persistence of Pasteurella multocida serovars and genotypes in fowl cholera outbreaks. Avian Pathol 42, 581-585.
14
St Michael, F., Harper, M., Parnas, H., John, M., Stupak, J., Vinogradov, E., et al., 2009. Structural and genetic basis for the serological differentiation of Pasteurella multocida Heddleston serotypes 2 and 5. J Bacteriol 191, 6950-6959.
15
Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J., Adler, B., 2001. Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system. J Clin Microbiol 39, 924-929.
16
Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M., Dawkins, H.J., 1998. Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates. J Clin Microbiol 36, 1096-1100.
17
Wilkie, I.W., Harper, M., Boyce, J.D., Adler, B., 2012. Pasteurella multocida: diseases and pathogenesis. Curr Top Microbiol Immunol 361, 1-22.
18
Wilson, M.A., Morgan, M.J., Barger, G.E., 1993. Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates. J Clin Microbiol 31, 255-259.
19
ORIGINAL_ARTICLE
The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.
https://archrazi.areeo.ac.ir/article_111601_97eff51c98a27271fec42421e8fd8759.pdf
2017-09-01
173
179
10.22092/ari.2017.111601
Infectious bursal disease
Gumboro Disease
Chicken embryo fibroblast
Ovine lymphoid origin cell line
Cell characterization
S.
Zandieh
saba.1204@gmail.com
1
Department of Genetics, Islamic Azad University, Ahar, Iran.
AUTHOR
Mohsen
Lotfi
m.lotfi@rvsri.ac.ir
2
Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
LEAD_AUTHOR
M.
Kamalzadeh
m.kamalzadeh@rvsri.ac.ir
3
Head of viral vaccine QC laboratory
AUTHOR
N.
Shiri
n.shiri@rvsri.ac.ir
4
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
AUTHOR
E.
Parmour
e.parmoor@rvsri.ac.ir
5
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
AUTHOR
A.
Eshaghi
eshaghi55@yahoo.com
6
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
AUTHOR
S.
Masoudi
s.masoudi@rvsri.ac.ir
7
AUTHOR
M. H.
Hablolvarid
8
Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
AUTHOR
A.
Shoushtari
hamid1342ir@yahoo.com
9
Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
AUTHOR
H.
Goudarzi
10
Research and diagnosis of poultry diseases management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
AUTHOR
S. M. J.
Taher Mofrad
smj_taheri@yahoo.com
11
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
AUTHOR
S.
Amanpour
saeidamanpour@yahoo.com
12
Imam Khomeini Hospital Cancer Institute, Tehran, Iran
AUTHOR
Arslan, A., Zima, J., 2011. Banded karyotype of the Konya wild sheep (Ovis orientalis anatolica Valenciennes, 1856) from Turkey. Comp Cytogenet 5, 81-89.
1
Berg, T.P.V.D., Eterradossi, N., Toquin, D., Meulemans, G., 2000. Infectious bursal disease (Gumboro disease). Rev Sci Tech OIE 19 527-543.
2
Butt, T.M., Rafique, R., Rauf, U., Khan, M.N., Nayyer, A., Abbas, Y., 2015. Adaption of infectious bursal disease virus (IBDV) on chicken embryo-fibroblast culture. Int J Agri Sci Res 4, 21-23.
3
Cooper, J.K., Sykes, G., King, S., Cottrill, K., Ivanova, N.V., Hanner, R., et al., 2007. Species identification in cell culture: a two-pronged molecular approach. In Vitro Cell Dev Biol Anim 43, 344-351.
4
Eterradossi, N., Saif, Y.M., 2013. Infectious bursal disease, Disease of poultry,, Wiley-Blackwell.
5
Freshney, R.I., 2011. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Wiley.
6
Hitchner, S.B., 1970. Infectivity of infectious bursal disease virus for embryonating eggs. Poult Sci 49, 511-516.
7
Hossain, S.A., Uddin, S.N., Rahman, M.S., Wadud, A., Khan, M.H., 2006. Propagation of infectious bursal disease (IBDV) in chicken embryo fibroblast cells. J Biol Sci 6, 146-149.
8
Ingrao, F., Rauw, F., Lambrecht, B., van den Berg, T., 2013. Infectious Bursal Disease: a complex host-pathogen interaction. Dev Comp Immunol 41, 429-438.
9
Mahgoub, H.A., Bailey, M., Kaiser, P., 2012. An overview of infectious bursal disease. Arch Virol 157, 2047-2057.
10
Muller, H., Islam, M.R., Raue, R., 2003. Research on infectious bursal disease--the past, the present and the future. Vet Microbiol 97, 153-165.
11
Muller, H., Mundt, E., Eterradossi, N., Islam, M.R., 2012. Current status of vaccines against infectious bursal disease. Avian Pathol 41, 133-139.
12
OIE Terrestrial Manual, 2012. Infectious bursal disease (Gumboro disease). pp. 548-565.
13
Rasool, M.H., Hussain, I., 2006. Preparation and evaluation of Vero-cell infectious bursal disease vaccine in Pakistan. Vaccine 24, 2810-2814.
14
Reed, L.J., Muench, H.A., 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27, 493-497.
15
Terasaki, K., Hirayama, H., Kasanga, C.J., Maw, M.T., Ohya, K., Yamaguchi, T., et al., 2008. Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus. J Vet Med Sci 70, 407-410.
16
ORIGINAL_ARTICLE
Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin
Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.
https://archrazi.areeo.ac.ir/article_111609_eaa7514b51ade19804e6ce5adb392c25.pdf
2017-09-01
181
187
10.22092/ari.2017.111609
Antioxidant activity
Crocin
Cytotoxicity
Grape Seed Extract
Phenytoin
N.
Razmaraii
nasserrazmaraii@gmail.com
1
AUTHOR
H.
Babaei
babaei42@yahoo.com
2
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
AUTHOR
A.
Mohajjel Nayebi
nayebia@yahoo.com
3
School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran
AUTHOR
S.
Ahdi Khosroshahi
shkhosroshahi@yahoo.com
4
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
AUTHOR
S.
Farajnia
farajnia@gmail.com
5
Drug Applied Research Center, Tabriz University of Medical Sciences
LEAD_AUTHOR
Ahn, H.S., Jeon, T.I., Lee, J.Y., Hwang, S.G., Lim, Y., Park, D.K., 2002. Antioxidative activity of persimmon and grape seed extract: in vitro and in vivo. Nutr Res 22, 1265-1273.
1
Alavizadeh, S.H., Hosseinzadeh, H., 2014. Bioactivity assessment and toxicity of crocin: a comprehensive review. Food Chem Toxicol 64, 65-80.
2
Anderson, B.O., Yip, C.H., Smith, R.A., Shyyan, R., Sener, S.F., Eniu, A., et al., 2008. Guideline implementation for breast healthcare in low-income and middle-income countries: overview of the Breast Health Global Initiative Global Summit 2007. Cancer 113, 2221-2243.
3
Aune, D., Chan, D.S., Vieira, A.R., Rosenblatt, D.A.N., Vieira, R., Greenwood, D.C., Norat, T., 2012. Dietary compared with blood concentrations of carotenoids and breast cancer risk: a systematic review and meta-analysis of prospective studies. Am J Clin Nutr. 034165.
4
Balasundram, N., Sundram, K., Samman, S., 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence and potential uses. Food Chem 99, 191-203.
5
Bruno, E., Gargano, G., Villarini, A., Traina, A., Johansson, H., Mano, M.P., et al., 2016. Adherence to WCRF/AICR cancer prevention recommendations and metabolic syndrome in breast cancer patients. Int J Cancer 138, 237-244.
6
Correa, J.D., Queiroz-Junior, C.M., Costa, J.E., Teixeira, A.L., Silva, T.A., 2011. Phenytoin-induced gingival overgrowth: a review of the molecular, immune and inflammatory features. ISRN Dent 2011, 497850.
7
Dinicola, S., Cucina, A., Antonacci, D., Bizzarri, M., 2014. Anticancer effects of grape seed extract on human cancers: a review. J Carcinogenesis MutaS8: 005 .
8
Duan, X.J., Zhang, W.W., Li, X. M., Wang, B.G., 2006. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem 95, 37-43.
9
Ferlay, J., Shin, H.R., Bray, F., Forman, D., Mathers, C., Parkin, D.M., 2010. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127, 2893-2917.
10
Fernández, J.A., 2006. Anticancer properties of saffron, Crocus sativus Linn. Advances Phytomedicine 2, 313-330.
11
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., et al., 2002. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113, 71-88.
12
Kumar, S., Pandey, A.K., 2013. Chemistry and Biological Activities of Flavonoids: An Overview. Sci World J 2013, 1-17.
13
Mazzio, E.A., Soliman, K.F., 2009. In vitro screening for the tumoricidal properties of international medicinal herbs. Phytotherapy Res 23, 385-398.
14
Mousavi, S.H., Moallem, S.A., Mehri, S., Shahsavand, S., Nassirli, H., Malaekeh-Nikouei, B., 2011. Improvement of cytotoxic and apoptogenic properties of crocin in cancer cell lines by its nanoliposomal form. Pharm biol 49, 1039-1045.
15
Nassiri-Asl, M., Hosseinzadeh, H., 2009. Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds. Phytotherapy Res: PTR 23, 1197-1204.
16
Newman, D.J., Cragg, G.M., 2007. Natural Products as Sources of New Drugs over the Last 25 Years⊥. J Nat Prod 70, 461-477.
17
Pereira, C.A., Alchorne Ade, O., 2010. Assessment of the effect of phenytoin on cutaneous healing from excision of melanocytic nevi on the face and on the back. BMC Dermatol 10, 7.
18
Razmaraii, N., Babaei, H., Mohajjel Nayebi, A., Assadnassab, G., Ashrafi Helan, J., Azarmi, Y., 2016a. Crocin treatment prevents doxorubicin-induced cardiotoxicity in rats. Life Sci 157, 145-151.
19
Razmaraii, N., Babaei, H., Nayebi, A.M., Asadnasab, G., Helan, J.A., Azarmi, Y., 2016b. Cardioprotective Effect of Phenytoin on Doxorubicin-Induced Cardiac Toxicity in a Rat Model. J Cardiovasc Pharmacol 69, 237-245.
20
Rizzon, P., Di Biase, M., Favale, S., Visani, L., 1987. Class 1B agents lidocaine, mexiletine, tocainide, phenytoin. Eur Heart J 8, 21-25.
21
Sen, S., Chakraborty, R., 2011. The role of antioxidants in human health. Oxidative stress: diagnostics, prevention and therapy 1083, 1-37.
22
Sung, J., Lee, J., 2010. Antioxidant and antiproliferative activities of grape seeds from different cultivars. Food Sci Biotechnol 19, 321-326.
23
World Health Organization, 2002. WHO traditional medicine strategy 2002-2005.
24
World Health Organization, 2014. Estimated cancer incidence, mortality and prevalence worldwide in 2012.
25
ORIGINAL_ARTICLE
Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran
Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect T.annulata DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for Theileria spp. and T.orientalis amplification, respectively. The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both T.annulata and T.orientalis. Out of 291 buffaloes, four samples (1.4%) were infected by Theileria genotypes, and two buffaloes (0.7%) were infected only by T.orientalis. The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that T.orientalis may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with T.orientalis for the first time.
https://archrazi.areeo.ac.ir/article_111605_c7d2f7bd39cc7dad9904aa5ed56e8e0a.pdf
2017-09-01
189
195
10.22092/ari.2017.111605
Buffalo
Cattle
Iran
PCR
Theileria orientalis
B.
Narimani
narimani.b@gmail.com
1
Department of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
N.
Hoghooghi-Rad
hoghooghirad@gmail.com
2
Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
LEAD_AUTHOR
P.
Shayan
shayanp@ut.ac.ir
3
Department of Pathobiology, Faculty of Veterinary Science, Tehran University, Tehran, Iran
AUTHOR
S.
Rahbari
rahbaris@ut.ac.ir
4
Department of Microbiology, Faculty of Specialized Veterinary Science, Branch of Science and Research, Islamic Azad University, Tehran, Iran
AUTHOR
Ahmed, J.S., Luo, J., Schnittger, L., Seitzer, U., Jongejan, F., Yin, H., 2006. Phylogenetic position of small-ruminant infecting piroplasms. Ann N Y Acad Sci 1081, 498-504.
1
Akbari, j., Javanbakht, J., Tavassoli, M., Tabatabai, M., Shafiei, R., 2012. Molecular survey of Theileria annulata in cattle by PCR-RFLP method in Iran. Bacteriol Parasitol 3, 1-6.
2
Aktas, M., Altay, K., Dumanli, N., 2006. A molecular survey of bovine Theileria parasites among apparently healthy cattle and with a note on the distribution of ticks in eastern Turkey. Vet Parasitol 138, 179-185.
3
Almeria, S., Castellà, J., Ferrer, D., Ortuño, A., Estrada-Peña, A., Gutiérrez, J.F., 2001. Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection. Veterinary Parasitology 99, 249-259.
4
Altay, K., Aydin, M.F., Dumanli, N., Aktas, M., 2008. Molecular detection of Theileria and Babesia infections in cattle. Vet Parasitol 158, 295-301.
5
Dumanli, N., Aktas, M., Cetinkaya, B., Cakmak, A., Koroglu, E., Saki, C.E., et al., 2005. Prevalence and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol 127, 9-15.
6
Garcı́a-Garcı́a, J.C., Montero, C., Redondo, M., Vargas, M., Canales, M., Boue, O., et al., 2000. Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus. Vaccine 18, 2275-2287.
7
Ghaemi, P., Hoghooghi-Rad, N., Shayan, P., Eckert, B., 2012. Detection of Theileria orientalis in Iran by semi-nested PCR. Parasitol Res 110, 527-531.
8
Hashemi-Fesharki, R., 1988. Control of Theileria annulata in Iran. Parasitology Today 4, 36-40.
9
Hashemi-Fesharki, R., Habibi, G.R., Ahourai, P., 1998. Delayed type hypersensitivity theilerin test in cattle vaccinated against Theileria annulata infection. Veterinary Parasitology 75, 261-263.
10
Hayashida, K., Hara, Y., Abe, T., Yamasaki, C., Toyoda, A., Kosuge, T., et al., 2012. Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation. MBio 3, e00204-00212.
11
Hoghooghi-Rad, N., Ghaemi, P., Shayan, P., Eckert, B., Sadr-Shirazi, N., 2011. Detection of native carrier cattle infected with Theileria annulata by semi-nested PCR in Golestan province of Iran. World Appl Sci J 12 317-323.
12
Mehlhorn, H., 2008. Encyclopedia of Parasitology: A-M, Springer.
13
Noaman, V., 2013. A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran. J Parasit Dis 37, 208-210.
14
Ota, N., Mizuno, D., Kuboki, N., Igarashi, I., Nakamura, Y., Yamashina, H., et al., 2009. Epidemiological survey of Theileria orientalis infection in grazing cattle in the eastern part of Hokkaido, Japan. J Vet Med Sci 71, 937-944.
15
Rahbari, S., Nabian, S., Shayan, P., 2007. Primary report on distribution of tick fauna in Iran. Parasitol Res 101 Suppl 2, S175-177.
16
Rodriguez-Valle, M., Taoufik, A., Valdes, M., Montero, C., Ibrahin, H., Hassan, S.M., et al., 2012. Efficacy of Rhipicephalus (Boophilus) microplus Bm86 against Hyalomma dromedarii and Amblyomma cajennense tick infestations in camels and cattle. Vaccine 30, 3453-3458.
17
Safarpour Dehkordi, F., Parsaei, P., Saberian, S., Moshkelani, S., Hajshafiei, P., Hoseini, S.R., 2012. Prevalence study of T.annulata by comparison of four diagnostic techniques in South-west Iran. Bulg J Vet 2, 123-130.
18
Savini, G., Onuma, M., Scaramozzino, P., Kakuda, T., Semproni, G., Langella, V., 1998. First report of Theileria sergenti and T. buffeli/orientalis in cattle in Italy. Ann N Y Acad Sci 849, 404-407.
19
Shayan, P., Jafari, S., Fattahi, R., Ebrahimzade, E., Amininia, N., Changizi, E., 2016. Identification and characterization of Theileria ovis surface protein (ToSp) resembled TaSp in Theileria annulata. Parasitol Res 115, 1893-1899.
20
Shayan, P., Rahbari, S., 2005. Simultaneous differentiation between Theileria spp. and Babesia spp. on stained blood smear using PCR. Parasitol Res 97, 281-286.
21
Uilenberg, G., 1981. Theilerial Species of Domestic Livestock. In: Irvin, A.D., Cunningham, M.P., Young, A.S. (Eds.), Advances in the Control of Theileriosis: Proceedings of an International Conference held at the International Laboratory for Research on Animal Diseases in Nairobi, 9–13th February, 1981, Springer Netherlands, Dordrecht, pp. 4-37.
22
Uilenberg, G., Hashemi-Fesharki, R., 1984. Theileria orientalis in Iran. Vet Q 6, 1-4.
23
Willadsen, P., Smith, D.O.N., Cobon, G., McKenna, R.V., 1996. Comparative vaccination of cattle against Boophilus microplus with recombinant antigen Bm86 alone or in combination with recombinant Bm91. Parasite Immunology 8, 241-246.
24
Yokoyama, N., Ueno, A., Mizuno, D., Kuboki, N., Khukhuu, A., Igarashi, I., et al., 2011. Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa prefectures of Japan. J Vet Med Sci 73, 305-312.
25
ORIGINAL_ARTICLE
Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran
Hepatozoon species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect Hepatozoon canis in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of 104 blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, 18S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of 897 bp for all the positive samples. Twenty-four out of the 104 (23.07%) samples were found to be positive for H. canis. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of 99% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of H. canis from Iran.
https://archrazi.areeo.ac.ir/article_108389_5b5fffbfaebb8b3e8171aa9a5440e53d.pdf
2017-09-01
197
201
10.22034/ari.2017.108389
Hepatozoon canis
PCR
Dog
Ardabil
Iran
A.
Dalimi
dalimi4@yahoo.com
1
Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
LEAD_AUTHOR
F.
Jameie
f.jameie@modares.ac.ir
2
Faculty of Medical Sciences, Tarbiat Modares University
AUTHOR
A.
Mohammadiha
anita_mohammadiha@yahoo.com
3
Faculty of Medical Sciences, Tarbiat Modares University
AUTHOR
M.
Barati
mbaratim@gmail.com
4
Infectious diseases Research Center, Aja University of Medical Sciences
AUTHOR
S.
Molaei
smolaie83@gmail.com
5
Faculty of Medical Sciences, Tarbiat Modares University
AUTHOR
Aktas, M., Ozubek, S., Altay, K., Balkaya, I., Utuk, A.E., Kirbas, A., et al., 2015. A molecular and parasitological survey of Hepatozoon canis in domestic dogs in Turkey. Vet Parasitol 209, 264-267.
1
Aktas, M., Ozubek, S., Ipek, D.N., 2013. Molecular investigations of Hepatozoon species in dogs and developmental stages of Rhipicephalus sanguineus. Parasitol Res 112, 2381-2385.
2
Baneth, G., Barta, J.R., Shkap, V., Martin, D.S., Macintire, D.K., Vincent-Johnson, N., 2000. Genetic and antigenic evidence supports the separation of Hepatozoon canis and Hepatozoon americanum at the species level. J Clin Microbiol 38, 1298-1301.
3
Baneth, G., Samish, M., Alekseev, E., Aroch, I., Shkap, V., 2001a. Transmission of Hepatozoon canis to Dogs by Naturally-Fed or Percutaneously-Injected Rhipicephalus sanguineus Ticks. J Parasitol 87, 606-611.
4
Baneth, G., Samish, M., Shkap, V., 2007. Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). J Parasitol 93, 283-299.
5
Baneth, G.A.D., Samish, M., Alekseev, E., Aroch, I., Shkap, V., 2001b. Transmission of Hepatozoon canis to Dogs by Naturally-Fed or Percutaneously-Injected Rhipicephalus sanguineus Ticks. J Parasitol 87, 606-611.
6
Christophers, S.R., 2016. Leucocytozoon Canis (Classic Reprint), Fb &C Limited.
7
Criado-Fornelio, A., Martinez-Marcos, A., Buling-Saraña, A., Barba-Carretero, J.C., 2003. Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe. Vet Parasitol 114, 173-194.
8
Criado-Fornelio, A., Rey-Valeiron, C., Buling, A., Barba-Carretero, J.C., Jefferies, R., Irwin, P., 2007. New advances in molecular epizootiology of canine hematic protozoa from Venezuela, Thailand and Spain. Vet Parasitol 144, 261-269.
9
Dissanaike, A.S., 1961. Hepatozoon canis infection in dogs in Ceylon. Ceylon Vet J 9, 144–145.
10
Elias, E., Homans, P.A., 1988. Hepatozoon canis infection in dogs: clinical and haematological findings; treatment. Journal of Small Anim Pract 29, 55-62.
11
Gomes, P.V., Mundim, M.J., Mundim, A.V., de Avila, D.F., Guimaraes, E.C., Cury, M.C., 2010. Occurrence of Hepatozoon sp. in dogs in the urban area originating from a municipality in southeastern Brazil. Vet Parasitol 174, 155-161.
12
Gondim, L.s.F.P., Kohayagawa, A., Alencar, N.X., Biondo, A.W., Takahira, R.K., Franco, S.R.V., 1998. Canine hepatozoonosis in Brazil: description of eight naturally occurring cases. Vet Parasitol 74, 319-323.
13
Gonen, L., Strauss-Ayali, D., Shkap, V., Vincent-Johnson, N., Macintire, D.K., Baneth, G., 2004. An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis. Vet Parasitol 122, 131-139.
14
Inokuma, H., Ohno, K., Yamamoto, S., 1999. Serosurvey of Ehrlichia canis and Hepatozoon canis infection in dogs in Yamaguchi Prefecture, Japan. J Vet Med Sci 61, 1153-1155.
15
Inokuma, H., Okuda, M., Ohno, K., Shimoda, K., Onishi, T., 2002. Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Veterinary Parasitology 106, 265-271.
16
James, S.P., 1905. On a parasite found in the white corpuscles of the blood of dogs. Sci Mem Offrs Med Sanit Deps India 14, 1-12.
17
Khazeni, A., Telmadarraiy, Z., Oshaghi, A., Mohebali, M., Zarei, Z., Abtahi, S.M., 2013. Molecular detection of <i>Ehrlichia canis</i> in ticks population collected on dogs in Meshkin-Shahr, Ardebil Province, Iran. J Biomed Sci Engin 06, 5.
18
Khoshnegah, J., Mohri, M., Movassaghi, A.R., Mehrjerdi , H.K., 2009. The first report of Hepatozoon canis infection of a dog in Iran. Com Clin Pathol 18, 455-458.
19
Murata, T., Shiramizu, K., Hara, Y., Inoue, M., Shimoda, K., Nakama, S., 1991. First case of Hepatozoon canis infection of a dog in Japan. J Vet Med Sci 53, 1097-1099.
20
O’Dwyer, L.H., Massard, C.L., Pereira de Souza, J.C., 2001.Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil. Veterinary Parasitology 94, 143-150.
21
Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M.S., Stanneck, D., Decaprariis, D., et al., 2011. Diagnosis of Hepatozoon canis in young dogs by cytology and PCR. Parasit Vectors 4, 55.
22
Paludo, G.R., Dell’Porto, A., de Castro e Trindade, A.R., McManus, C., Friedman, H., 2003. Hepatozoon spp.: report of some cases in dogs in Brası́lia, Brazil. Vet Parasitol 118, 243-248.
23
Pawar, R.M., Poornachandar, A., Srinivas, P., Rao, K.R., Lakshmikantan, U., Shivaji, S., 2012. Molecular characterization of Hepatozoon spp. infection in endangered Indian wild felids and canids. Vet Parasitol 186, 475-479.
24
Rahmani Amoli, A.A., Khoshnegah, J., Razmi, G.R., 2012. A preliminary parasitological survey of Hepatozoon spp. infection in dogs in Mashhad, Iran. Iranian J Parasitol 7, 99-103.
25
Rajamanickam, C., Wiesenhutter, E., Zin, F.M., Hamid, J., 1985. The incidence of canine haematozoa in peninsular Malaysia. Vet Parasitol 17, 151-157.
26
Razmi, G.R., Naghibi, A., Aslani, M.R., Dastjerdi, K., Hossieni, H., An epidemiological study on Babesia infection in small ruminants in Mashhad suburb, Khorasan province, Iran. Small Rumin Res 50, 39-44.
27
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28
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ORIGINAL_ARTICLE
The Fauna and Active Season of Mosquitoes in West of Fars Province, Southwest of Iran
Culicidae are highly important for public health as they can be vectors of diseases and are responsible for a wide spectrum of infections. Five collection sites were selected randomly with regards to existing facilities in Firouzabad County. For collecting larvae and total catch for adult mosquitoes, sampling was carried out by dipping technique for collecting larvae and total catch for adult mosquitoes. A total of 689 adults and 1313 larvae of Culicidae were collected, of which 3 genera and 6 species of Culicidae were recognized, namely, Anopheles superpictus, Anopheles d’thali, Culex sinaiticus, Culex theileri, Culex mimeticus, and Culiseta longiareolata. Cx. theileri was the most frequent Culicidae collected at Firouzabad, with a total of 613 and 247 larval and adult specimens, respectively. The highest number of mosquitoes was collected in June (31.1%) and the lowest in May (3.4%). The mean temperatures in June and May were 31.3˚C and 28.2˚C, respectively. We found some vectors that are of medical and veterinary importance; our results could be applied in vector control programs that aim at eradication or control of mosquitoes in this area.
https://archrazi.areeo.ac.ir/article_111603_9f639dd4d19caf45e13b7d75b026e69f.pdf
2017-09-01
203
208
10.22092/ari.2017.111603
Culicidae
Monthly frequency
Fars
Southwestern Iran
Z.
Soltani
keshavarzd25@gmail.com
1
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
D.
Keshavarzi
forensicentomology@yahoo.com
2
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
M.
Ebrahimi
mostafa212@yahoo.com
3
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
A.
Soltani
abu2sol@yahoo.com
4
Research Centre for Health Sciences, Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
M. J.
Moemenbellah-Fard
momenbf@yahoo.com
5
Department of Medical Entomology and Vector Control, Research Centre for Health Sciences, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
AUTHOR
F.
Soltani
6
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
H.
Faramarzi
faramarzih28@gmail.com
7
4Department of Community Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
AUTHOR
K.
Amraee
amraee-k@ajums.ac.ir
8
Department of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
A.
Elyasigomari
elyasi.arezo@yahoo.com
9
5Department of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
AUTHOR
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