%0 Journal Article %T Study on Cryo-preservation of Theileria annulata Schizont Infected Cell Line Vaccine Strain S15 %J Archives of Razi Institute %I Razi Vaccine & Serum Research Institute %Z 0365-3439 %A Esmaeil Nia, K. %A Hatami, A. %A Bozorgi, S. %A Habibi, G. %A Afshari, A. %D 2012 %\ 12/01/2012 %V 67 %N 2 %P 167-172 %! Study on Cryo-preservation of Theileria annulata Schizont Infected Cell Line Vaccine Strain S15 %K Cryo %K PRESERVATION %K Theileria annulata %K Sorbitol %K sucrose %K gelatin %K Glycerol %R 10.22092/ari.2016.103902 %X The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the conventional cryo-preservative medium containing 5% PEG treated Bovine Serum and 10% glycerol successfully cryo-preserved cell as efficiently as the standard medium containing 80% FBS and 10% glycerol and was superior to all thirteen different experimental cryo-preservative media. A crucial result was obtained among the thirteen different serum-free media which showed that the 0.2M sorbitol+10% glycerol method yielded cells with viability of 54% and good plating efficiency results. It was concluded that the conventional method would be the best and ideal for the cryo-preservation and storage of Theileria annulata-infected cells for vaccine purposes to date. %U https://archrazi.areeo.ac.ir/article_103902_5b6e269f08f5e64c9fe02054d9ebdba0.pdf