eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
147
157
10.22092/ari.2017.111611
111611
مقالات پژوهشی
Analysis of variations, structures, and phylogenic characteristics of bovine leukocyte antigen DRB3 exon2
M. M. Ranjbar
mm.ranjbar.phd@gmail.com
1
S. Ataei
ataei111@hotmail.com
2
Gh. Nikbakht Brujeni
nikbakht@ut.ac.ir
3
S. Golabdar
golabdar@ut.ac.ir
4
Department of Viral Animal Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Department of Avian Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular andevolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustrate some novelpractical evidence on interspecies diversity, the resistance /susceptibility points in cattle breeding, and vaccinedesign. Initially, BoLA-DRB3.2* alleles and orthologous exons in the selected livestock were retrieved andchecked. In the next step, the secondary/tertiary structure of BoLA-DRB3.2*24 gene product was modeled andvalidated. Then, hypervariable regions (HVRs) of alleles were identified by hybrid approaches. In the last step,interspecies relationship, allele’s phylogeny/grouping, and estimate of average evolutionary divergence wereexplored. Shannon entropy variation analysis showed eight HVRs and three semi-variable regions in BoLADRB3.2*alleles. These HVRs were present in all the three sub-structures and dominantly existed in alpha helix.In addition, strong relationships and little diversity were noted in phylogenetic trees of cattle, buffaloes, sheep,and goats. Furthermore, there was some evidence on divergence of DRB3 before speciation among thementioned species and possibility of cross prediction resistance/susceptibility alleles. Finally, DRB3 alleles weregrouped into seven clusters, and older and newer alleles were identified. The results show that similar studiesshould be done in other animals to better understand the nature of the DRB3 attributes.
https://archrazi.areeo.ac.ir/article_111611_50130e38448fdbd64a0f96493963014f.pdf
BoLA-DRB3.2
Cattle
Variation
modeling
phylogeny
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
159
164
10.22092/ari.2017.111610
111610
مقالات پژوهشی
Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats
S. A. Pourbakhsh
poursaba@yahoo.com
1
A. R. Abtin
dvm_alirezaabtin@yahoo.com
2
A. Ashtari
abbass.ashtari@gmail.com
3
B. Kheirkhah
babakkheirkhah@yahoo.com
4
M. A. Bayatzadeh
ma.bayatzadeh@yahoo.com
5
Salimeh Ahangran
salroze@yahoo.com
6
Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Master
Assistant Professor
Master
Bachelor
Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease ingoats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semensamples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented forM. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was appliedto detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bpfragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed forboth the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positiveand typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. Inaddition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the sampleswere shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M.agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended toconcern semen as one of the significant sources for this pathogen and the possibility for transmission to thefemale goats through semen is highlighted. Moreover, presence of this microorganism in semen could beinvolved in infertility of goat population.
https://archrazi.areeo.ac.ir/article_111610_714d06eb229f24c70e07977dcce074e6.pdf
Goat buck
Lipoprotein gene
Mycoplasma agalactiae
Semen
16srRNA
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
165
171
10.22092/ari.2017.111607
111607
مقالات پژوهشی
LPS-PCR typing of ovine Pasteurella multocida isolates from Iran based on (L1 to L8) outer core biosynthesis loci
S. F. Mirhaghgoye Jalali
jalali.fahimeh1950@gmail.com
1
A.R. Jabbari
a.jabbari@rvsri.ac.ir
2
M. Esmael Zad
m.esmaelizad@rsri.ac.ir
3
Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
assistant professor razi institute
Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic typing systems.The aim of this study was to identify the LPS genotypes of the ovine P. multocida isolates obtained from pneumonia cases in Iran. The LPS genotype of the isolates was determined using eight specific primers for LPS outer core biosynthesis loci. The LPS genes were amplified by polymerase chain reaction (PCR), then they were sequenced and compared to the sequences registered in the GenBank. Of the 32 ovine P. multocida isolates tested, 21 (65.62%) isolates belonged to genotype L6, 9 (28.12%) isolates contained genotype L3, 1 (3.12%) isolate had both L3 and L6 loci, and 1 (3.12%) isolate remained untypeable. The LPS-PCR was able to type 31 of 32 field ovine isolates from Iran. According to the phylogenetic analysis, L3 genotype isolates were grouped into two distinct lineages. LPS gene sequences among L6 genotypes of ovine P. multocida isolates from Iran and the related sequences in the GenBank were highly similar (>99.5%). LPS-PCR is an accurate genotyping method that was able to classify P. multocida strains into one of the eight distinct LPS genotypes.
https://archrazi.areeo.ac.ir/article_111607_ec494786bf8c99c264e682d2b81fb9f7.pdf
P. multocida
Sheep
LPS outer core
PCR-typing
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
173
179
10.22092/ari.2017.111601
111601
مقالات پژوهشی
The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain
S. Zandieh
saba.1204@gmail.com
1
Mohsen Lotfi
m.lotfi@rvsri.ac.ir
2
M. Kamalzadeh
m.kamalzadeh@rvsri.ac.ir
3
N. Shiri
n.shiri@rvsri.ac.ir
4
E. Parmour
e.parmoor@rvsri.ac.ir
5
A. Eshaghi
eshaghi55@yahoo.com
6
S. Masoudi
s.masoudi@rvsri.ac.ir
7
M. H. Hablolvarid
8
A. Shoushtari
hamid1342ir@yahoo.com
9
H. Goudarzi
10
S. M. J. Taher Mofrad
smj_taheri@yahoo.com
11
S. Amanpour
saeidamanpour@yahoo.com
12
Department of Genetics, Islamic Azad University, Ahar, Iran.
Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
Head of viral vaccine QC laboratory
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
Quality control management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
Research and diagnosis of poultry diseases management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Imam Khomeini Hospital Cancer Institute, Tehran, Iran
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.
https://archrazi.areeo.ac.ir/article_111601_97eff51c98a27271fec42421e8fd8759.pdf
Infectious bursal disease
Gumboro Disease
Chicken embryo fibroblast
Ovine lymphoid origin cell line
Cell characterization
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
181
187
10.22092/ari.2017.111609
111609
مقالات پژوهشی
Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin
N. Razmaraii
nasserrazmaraii@gmail.com
1
H. Babaei
babaei42@yahoo.com
2
A. Mohajjel Nayebi
nayebia@yahoo.com
3
S. Ahdi Khosroshahi
shkhosroshahi@yahoo.com
4
S. Farajnia
farajnia@gmail.com
5
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
Drug Applied Research Center, Tabriz University of Medical Sciences
Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.
https://archrazi.areeo.ac.ir/article_111609_eaa7514b51ade19804e6ce5adb392c25.pdf
Antioxidant activity
Crocin
Cytotoxicity
Grape Seed Extract
Phenytoin
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
189
195
10.22092/ari.2017.111605
111605
مقالات کوتاه
Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran
B. Narimani
narimani.b@gmail.com
1
N. Hoghooghi-Rad
hoghooghirad@gmail.com
2
P. Shayan
shayanp@ut.ac.ir
3
S. Rahbari
rahbaris@ut.ac.ir
4
Department of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Pathobiology, Faculty of Veterinary Science, Tehran University, Tehran, Iran
Department of Microbiology, Faculty of Specialized Veterinary Science, Branch of Science and Research, Islamic Azad University, Tehran, Iran
Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect T.annulata DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for Theileria spp. and T.orientalis amplification, respectively. The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both T.annulata and T.orientalis. Out of 291 buffaloes, four samples (1.4%) were infected by Theileria genotypes, and two buffaloes (0.7%) were infected only by T.orientalis. The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that T.orientalis may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with T.orientalis for the first time.
https://archrazi.areeo.ac.ir/article_111605_c7d2f7bd39cc7dad9904aa5ed56e8e0a.pdf
Buffalo
Cattle
Iran
PCR
Theileria orientalis
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
197
201
10.22034/ari.2017.108389
108389
مقالات کوتاه
Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran
A. Dalimi
dalimi4@yahoo.com
1
F. Jameie
f.jameie@modares.ac.ir
2
A. Mohammadiha
anita_mohammadiha@yahoo.com
3
M. Barati
mbaratim@gmail.com
4
S. Molaei
smolaie83@gmail.com
5
Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Faculty of Medical Sciences, Tarbiat Modares University
Faculty of Medical Sciences, Tarbiat Modares University
Infectious diseases Research Center, Aja University of Medical Sciences
Faculty of Medical Sciences, Tarbiat Modares University
Hepatozoon species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect Hepatozoon canis in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of 104 blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, 18S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of 897 bp for all the positive samples. Twenty-four out of the 104 (23.07%) samples were found to be positive for H. canis. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of 99% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of H. canis from Iran.
https://archrazi.areeo.ac.ir/article_108389_5b5fffbfaebb8b3e8171aa9a5440e53d.pdf
Hepatozoon canis
PCR
Dog
Ardabil
Iran
eng
Razi Vaccine & Serum Research Institute
Archives of Razi Institute
0365-3439
2008-9872
2017-09-01
72
3
203
208
10.22092/ari.2017.111603
111603
مقالات کوتاه
The Fauna and Active Season of Mosquitoes in West of Fars Province, Southwest of Iran
Z. Soltani
keshavarzd25@gmail.com
1
D. Keshavarzi
forensicentomology@yahoo.com
2
M. Ebrahimi
mostafa212@yahoo.com
3
A. Soltani
abu2sol@yahoo.com
4
M. J. Moemenbellah-Fard
momenbf@yahoo.com
5
F. Soltani
6
H. Faramarzi
faramarzih28@gmail.com
7
K. Amraee
amraee-k@ajums.ac.ir
8
A. Elyasigomari
elyasi.arezo@yahoo.com
9
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
Research Centre for Health Sciences, Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Medical Entomology and Vector Control, Research Centre for Health Sciences, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Communicable Disease Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
4Department of Community Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5Department of Medical Entomology and Vector Control, School of Public Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Culicidae are highly important for public health as they can be vectors of diseases and are responsible for a wide spectrum of infections. Five collection sites were selected randomly with regards to existing facilities in Firouzabad County. For collecting larvae and total catch for adult mosquitoes, sampling was carried out by dipping technique for collecting larvae and total catch for adult mosquitoes. A total of 689 adults and 1313 larvae of Culicidae were collected, of which 3 genera and 6 species of Culicidae were recognized, namely, Anopheles superpictus, Anopheles d’thali, Culex sinaiticus, Culex theileri, Culex mimeticus, and Culiseta longiareolata. Cx. theileri was the most frequent Culicidae collected at Firouzabad, with a total of 613 and 247 larval and adult specimens, respectively. The highest number of mosquitoes was collected in June (31.1%) and the lowest in May (3.4%). The mean temperatures in June and May were 31.3˚C and 28.2˚C, respectively. We found some vectors that are of medical and veterinary importance; our results could be applied in vector control programs that aim at eradication or control of mosquitoes in this area.
https://archrazi.areeo.ac.ir/article_111603_9f639dd4d19caf45e13b7d75b026e69f.pdf
Culicidae
Monthly frequency
Fars
Southwestern Iran