@article { author = {Zandieh, S. and Lotfi, Mohsen and Kamalzadeh, M. and Shiri, N. and Parmour, E. and Eshaghi, A. and Masoudi, S. and Hablolvarid, M. H. and Shoushtari, A. and Goudarzi, H. and Taher Mofrad, S. M. J. and Amanpour, S.}, title = {The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain}, journal = {Archives of Razi Institute}, volume = {72}, number = {3}, pages = {173-179}, year = {2017}, publisher = {Razi Vaccine & Serum Research Institute}, issn = {0365-3439}, eissn = {2008-9872}, doi = {10.22092/ari.2017.111601}, abstract = {Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.}, keywords = {Infectious bursal disease,Gumboro Disease,Chicken embryo fibroblast,Ovine lymphoid origin cell line,Cell characterization}, url = {https://archrazi.areeo.ac.ir/article_111601.html}, eprint = {https://archrazi.areeo.ac.ir/article_111601_97eff51c98a27271fec42421e8fd8759.pdf} }