CpG-DNA enhancement the immune elicited as adjuvant of foot- and- mouth disease vaccine
H.
Mahravani
author
M.
Daghighi
author
A.
Morshedi
author
text
article
2010
eng
In the present study the effect of the locally produced genetic adjuvant of ginea pig specific CpG-motif-containing oligodeoxynucleotide (CpG-ODN) in an inactivated FMD virus vaccine was evaluated. Boosting the ginea pigs with FMD vaccine along with CpG-ODN adjuvant produced relatively higher ratio (5-fold) of FMDV-specific IgG2a / IgG1 than those vaccinated in the absence of CpG-ODN. The neutralizing antibody (NA) titer induced by FMD vaccine along with CpG-ODN adjuvant was significantly higher (8-fold) than NA titer induced by the classical FMD vaccine in Alum adjuvant. The titer of NA and virus clearance from serum was consistently and significantly higher in animals primed with FMD vaccine and boosted by CpG-ODN than the classical FMD vaccine. The results of this study showed the potential of CpG-ODN as a genetic adjuvant to FMD vaccine in the development of Th1 responses.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
1
7
https://archrazi.areeo.ac.ir/article_103844_4899f911e62a363f0a8fe2b731063a03.pdf
dx.doi.org/10.22092/ari.2010.103844
Detection of Echinococcus granulosus infection in dogs
H.
Paykari
author
M.
Syavashi
author
Gh.R.
Motamedi
author
A.
Dalimi
author
M.H.
Hablolvarid
author
text
article
2010
eng
Cystic Echinococcosis is an important zoonosis in the sheep rising areas of Iran. To develop a simple and reliable diagnostic method for Echinococcus infection in definitive hosts, E. granulosus polyoclonal antibodies (PolyAbs) were prepared from adult worm in rabbit. A selected PoAb was used for coproantigen detection in faecal samples obtained from animals naturally infected with Echinococcus and compared with necropsy method. Finally, the sensitivity and specificity of the test were evaluated. The results indicated E. granulosus worms was detected in 36 (43%) of small intestine contents of dogs. The results obtained by CpAg- ELISA test showed 30 (36.14%) positive and 53 (63.86%) negative cases. The sensitivity and specificity of CpAg-ELISA test were evaluated 83.33% and 100% respectively. In conclusion, the present result suggests that, CpAg-ELISA is a valid test for detection of E. granulosus infection in living dogs. Thus it is appropriate to apply for epidemiological study.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
9
14
https://archrazi.areeo.ac.ir/article_103845_02cbbb1eea5917cb70a8d5ec61da7b20.pdf
dx.doi.org/10.22092/ari.2010.103845
Coccidiosis due to various species of Eimeria in the stunted and diarrheic native turkey poults: Pathology and morphological characterization of oocysts
S.
Rahbari
author
M.J.
Gharagozlou
author
O.
Dezfoulian
author
R.
Samani
author
text
article
2010
eng
Fecal samples of 60 turkey poults that showed chronic progressive symptoms like unthriftiness, loss ofweight, diarrhea were collected from the most rural areas with high rate of turkey population in north andwest part of country for intestinal protozoan parasites. According to the morphological characteristics, likeshape, presence or absence of micropyle, and/or polar granule, the 5 different types of eimerian oocyctswere diagnosed in the stool of infected birds, including E. adenoids, E. meleagridis, E. dispersa, Eimeriaspp (E. innocua or E. subrotunda) and E. meleagrimitis. Various life- cycle stages of Eimeria wereidentified in the epithelial lining of inflamed intestine of the affected turkey poults.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
15
19
https://archrazi.areeo.ac.ir/article_103846_aa5de0534396448f47bf7470f6043d74.pdf
dx.doi.org/10.22092/ari.2010.103846
Seroprevalence of Ehrlichia canis in dogs referred to Veterinary Hospital of Shahid Chamran University of Ahvaz, Iran
M.H.
Razi Jalali
author
B.
Mosallanejad
author
R.
Avizeh
author
A.R.
Alborzi
author
text
article
2010
eng
Canine ehrlichiosis is a zoonotic rickettsial disease transmitted by ticks. In the present study, 198 companion dogs of different ages were examined for serum antibody detection against Ehrlichia canis by means of immunochromatography assay. The dogs were selected among referred cases to Veterinary Hospital of Shahid Chamran University of Ahvaz, Southwestern Iran from November 2008 to March 2010. The studied dogs were classified based on age, sex, breed, region and season. Nineteen of 198 serum samples (9.6%) had antibody against E. canis. Prevalence was significantly higher in adult dogs more than 3 year-old (16.18%) (P= 0.002) and 1 – 3 years (11.86%) (P= 0.016) compared with young dogs less than 1 year-old (1.41%). Prevalence was higher in male dogs (10.62%) than female dogs (8.24%), in the summer (11.32%) and west region (11.11%). There were not significant differences between the prevalence of infection and host gender, season and region (P>0.05). Typical morulae of E. canis were observed in monocytes of four infected dogs (2.02%). Five out of 24 (20.83%) of the thrombocytopenic dogs and 14 out of 174 (8.05%) of the non-thrombocytopenic dogs were positive for ehrlichiosis. Of 19 seropositive dogs, six (31.58%) had anemia, four (21.05%) hypoalbuminemia and five (26.32%) leukopenia. There were not statistically significant differences between hematological findings and prevalence of infection (P> 0.05). This is the first report indicating the presence of E. canis in companion dogs of Ahvaz district. However, the sources of infection in these dogs were not clear. Finally, the role of companion dogs in the epizootiology of E. canis infection needs to be further explored.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
21
26
https://archrazi.areeo.ac.ir/article_103847_bb43816be19d943a49d76e1aa6921d63.pdf
dx.doi.org/10.22092/ari.2010.103847
The prevalence and intensity of Eimeria spp. infection in sheep of Malayer suburb, Iran
A.A.
Rezaei
author
M.
Yakhchali
author
text
article
2010
eng
The prevalence of Eimeria spp. infection and the intensity of fecal oocysts were determined in 250 sheep in Malayer suburb of Iran using flotation and sporulation techniques. The overall prevalence was 23.23% in which the young male sheep had the highest prevalence (37.61%) with the highest intensity (63.58%). There were no significant difference in the prevalence between male (27.9%) and female (22.93%) in all age groups (P>0.05). The young sheep had significantly higher oocysts counts than the other groups (P
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
27
32
https://archrazi.areeo.ac.ir/article_103848_9f56408ccd30471d37fb842f744fa301.pdf
dx.doi.org/10.22092/ari.2010.103848
Production of effective antivenin to treat cobra snake
H.
Zolfagharian
author
N.
Mohammadpour
author
A.
Hedayat
author
H.
Rabiei
author
A.
Akbari
author
Sh.
Teimorzadeh
author
text
article
2010
eng
Conventional treatment of Naja naja oxiana (NNO) envenoming requires large volumes of equine antivenin raised against NNO crude venom. The poor efficiency of this antivenin is assumed to be due to the high molecular weight non-toxic proteins, a strong immunogen, present in the crude venom. These proteins cause depression of antibody formation against the low molecular weight toxic components of venom. In the present study the low molecular weight lethal components were isolated from crude venom of NNO venom by sephadex G50 get filtration chromatography. A sera was prepared by immunizing horses with toxic fraction. One milliliter of this serum neutralized 1.8mg of NNO crude venom. This high titer antivenin is thus 2.2 times more potent than the sera obtained against NNO crude venom.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
33
37
https://archrazi.areeo.ac.ir/article_103849_3e986f3a2dbb695f2488c43af2e8cd2a.pdf
dx.doi.org/10.22092/ari.2010.103849
Antibody Detection to Feline Immunodeficiency virus (FIV) in stray cats in Ahvaz, southwestern Iran
N.
Samani
author
M.
Ghorbanpoor Najafabadi
author
M.R.
Seyfiabad Shapouri
author
R.
Avizeh
author
B.
Mosallanejad
author
text
article
2010
eng
The present study was performed to determine the prevalence of FIV in stray cat's population of Ahvaz different area. Serum samples were collected from 90 cats from 2005 to 2007. The studied cats were divided into two age groups (3 years) and based on clinical signs (such as lymphadenopathy, periodontal diseases, gingivitis, abscess and cashecsi) into two groups also. The results were analyzed using Fischer's exact test and Chi-square analysis. Prevalence to FIV antibodies in these cats was 15.55% (14 of 90) by means of ELISA Test Kit, indicating that this virus is present in the ecosystem. The infection had more prevalence in cats above 3 years (78.6% 11 of 14) compared with cats less than 3 years (21.4% 3 of 14). Statistical analysis showed significant difference between different age groups (P0.05). Three out of 12 cases (25%) which had clinical signs and 11 out of 78 cases (14.1%) which hadn’t clinical signs were seropositive. There was no significant difference between the two groups also (P>0.05). This study showed that FIV exist among cat's population of Ahvaz area and separation of companion and stray cats is very important for prevention of disease transmission to companion cats.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
39
44
https://archrazi.areeo.ac.ir/article_103850_c4ed20f1c1773ff4e49aa65cafb346fa.pdf
dx.doi.org/10.22092/ari.2010.103850
A Survey on horse Cryptosporidial Infection in Tehran Province
A.H.
Rezakhani
author
H.R.
Ferdowsi
author
M.R.
Asadi
author
S.J.
Mirian
author
text
article
2010
eng
To investigate the prevalence of Cryptosporidium infection in horses, a total of 200 fecal specimens were collected randomly from five farms in Tehran. Samples were taken during a year, 50 specimens each season. Of these specimens, 57 were obtained from Caspian Pony. The presence of oocysts in the samples was confirmed by modified Ziehl-Neelsen staining of direct smears of the fecal material from each horse. The results show a significant relationship between infection and season, so that the most infected cases were seen in summer. According to the results, the highest parasite activity is seen in warm weather, so care must be taken to prevent its transmission to human.
Archives of Razi Institute
Razi Vaccine & Serum Research Institute
0365-3439
65
v.
1
no.
2010
45
47
https://archrazi.areeo.ac.ir/article_103851_fecaddcbbe65031233b019fef5c9ceaf.pdf
dx.doi.org/10.22092/ari.2010.103851